Restriction map for
pFN21AE0924
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI AspEI AspI
AviII BbeI BbrPI BbsI BbvCI
BcgI BclI BglI BglII BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstF5I BstUI BstXI
BstZ17I CelII CfoI ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRV
EcoT22I FbaI FokI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KspI MaeII MamI
MfeI MluI MroI MslI MunI
MvnI NarI NcoI NdeI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 466 GGTACC G^GTACC
AcsI 909 GAATTC [GA]^AATT[CT]
AcyI 650 GGCGTC G[GA]^CG[CT]C
AflII 115 CTTAAG C^TTAAG
Alw44I 390 GTGCAC G^TGCAC
AocI 604 CCTGAGG CC^T[ACGT]AGG
ApaLI 390 GTGCAC G^TGCAC
ApoI 909 GAATTC [GA]^AATT[CT]
Asp700I 534 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 466 GGTACC G^GTACC
AspHI 390 GTGCAC G[AT]GC[AT]^C
AvaI 461 CCCGGG C^[CT]CG[GA]G
AvrII 147 CCTAGG C^CTAGG
BalI 965 TGGCCA TGG^CCA
BamHI 110 GGATCC G^GATCC
BanI 466 GGTACC G^G[CT][GA]CC
BanII 16 GGGCTC G[GA]GC[CT]^C
BfrI 115 CTTAAG C^TTAAG
BlnI 147 CCTAGG C^CTAGG
BsaHI 650 GGCGTC G[GA]^CG[CT]C
BsaWI 488 ACCGGA [AT]^CCGG[AT]
BseRI 339 CTCCTC GAGGAG(10/8)
BsgI 740 CTGCAC GTGCAG(16/14)
BsiHKAI 390 GTGCAC G[AT]GC[AT]^C
BsmI 86 GAATGC GAATGC(1/-1)
BsoBI 461 CCCGGG C^[CT]CG[GA]G
BstAPI 60 GCAGATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 404 GGTCACC G^GT[ACGT]ACC
BstPI 404 GGTCACC G^GT[ACGT]ACC
Bsu36I 604 CCTGAGG CC^T[ACGT]AGG
EarI 638 GAAGAG CTCTTC(1/4)
Eco81I 604 CCTGAGG CC^T[ACGT]AGG
EcoO65I 404 GGTCACC G^GT[ACGT]ACC
EcoRI 909 GAATTC G^AATTC
FauI 360 GCGGG CCCGC(4/6)
FseI 31 GGCCGGCC GGCCGG^CC
HgaI 651 GCGTC GACGC(5/10)
Hin1I 650 GGCGTC G[GA]^CG[CT]C
KpnI 466 GGTACC GGTAC^C
Ksp632I 638 GAAGAG CTCTTC(1/4)
MluNI 965 TGGCCA TGG^CCA
MscI 965 TGGCCA TGG^CCA
MseI 116 TTAA T^TAA
NaeI 32 GCCGGC GCC^GGC
NgoMIV 32 GCCGGC G^CCGGC
NheI 72 GCTAGC G^CTAGC
PpuMI 798 GGGTCCC [GA]G^G[AT]CC[CT]
SapI 638 GAAGAGC GCTCTTC(1/4)
SmaI 461 CCCGGG CCC^GGG
TaqI 234 TCGA T^CGA
Tru9I 116 TTAA T^TAA
TthHB8I 234 TCGA T^CGA
XmaCI 461 CCCGGG C^CCGGG
XmaI 461 CCCGGG C^CCGGG
XmnI 534 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 467 GTAC GT^AC
712 GTAC
AlwNI 700 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
985 CAGGCCCTG
BciVI 48 GGATAC GTATCC(6/5)
555 GTATCC
BfaI 73 CTAG C^TAG
148 CTAG
BpmI 422 CTCCAG CTGGAG(16/14)
529 CTGGAG
BsrFI 32 GCCGGC [GA]^CCGG[CT]
481 ACCGGC
BstYI 110 GGATCC [GA]^GATC[CT]
143 AGATCC
Cfr10I 32 GCCGGC [GA]^CCGG[CT]
481 ACCGGC
DpnII 111 GATC ^GATC
144 GATC
DraII 798 GGGTCCC [GA]G^G[ACGT]CC[CT]
986 AGGCCCT
DsaI 205 CCACGG C^C[GA][CT]GG
573 CCGTGG
EcoO109I 798 GGGTCCC [GA]G^G[ACGT]CC[CT]
986 AGGCCCT
EcoT14I 147 CCTAGG C^C[AT][AT]GG
715 CCAAGG
MaeI 73 CTAG C^TAG
148 CTAG
MboI 111 GATC ^GATC
144 GATC
MboII 638 GAAGA GAAGA(8/7)
503 TCTTC
MflI 110 GGATCC [GA]^GATC[CT]
143 AGATCC
NdeII 111 GATC ^GATC
144 GATC
RsaI 467 GTAC GT^AC
712 GTAC
Sau3AI 111 GATC ^GATC
144 GATC
StyI 147 CCTAGG C^C[AT][AT]GG
715 CCAAGG
Tsp509I 237 AATT ^AATT
910 AATT
XhoII 110 GGATCC [GA]^GATC[CT]
143 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DdeI EaeI
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIV PleI PvuII Sau96I ScrFI
SmlI TfiI TseI Tsp45I TspRI