(Circular) (Six-base) MAPSORT of: pFN21AE0924.seq Check: 765 from: 1 to: 5734 pFN21AE0924 5734 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3544 278 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3077 1937 Size: 1140 4594 Acc65I G'GTAC_C Cuts at: 2471 3055 2471 Size: 584 5150 AclI AA'CG_TT Cuts at: 1563 3861 4234 1563 Size: 2298 373 3063 Fragments arranged by size: 3063 2298 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 2328 3797 5181 5449 1632 Size: 641 55 1469 1384 268 1917 Fragments arranged by size: 1917 1469 1384 641 268 55 AflII C'TTAA_G Cuts at: 820 1017 2120 820 Size: 197 1103 4434 Fragments arranged by size: 4434 1103 197 AflIII A'CryG_T Cuts at: 1203 4633 5610 1203 Size: 3430 977 1327 Fragments arranged by size: 3430 1327 977 AgeI A'CCGG_T Cuts at: 4565 4565 Size: 5734 AhdI GACnn_n'nnGTC Cuts at: 4463 4463 Size: 5734 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5734 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2528 2560 2528 Size: 32 5702 AlwNI CAG_nnn'CTG Cuts at: 2710 2995 3477 5049 2710 Size: 285 482 1572 3395 Fragments arranged by size: 3395 1572 482 285 ApaLI G'TGCA_C Cuts at: 2395 3791 4947 2395 Size: 1396 1156 3182 Fragments arranged by size: 3182 1396 1156 ApoI r'AATT_y Cuts at: 1493 2914 3043 3269 3421 5530 1493 Size: 1421 129 226 152 2109 1697 Fragments arranged by size: 2109 1697 1421 226 152 129 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 2466 3059 1870 Size: 73 523 593 4545 Fragments arranged by size: 4545 593 523 73 AvrII C'CTAG_G Cuts at: 2152 2152 Size: 5734 BamHI G'GATC_C Cuts at: 2115 3064 3431 2115 Size: 949 367 4418 Fragments arranged by size: 4418 949 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2471 3055 4510 618 Size: 325 217 627 684 584 1455 1842 Fragments arranged by size: 1842 1455 684 627 584 325 217 BanII G_rGCy'C Cuts at: 1391 2025 3053 1391 Size: 634 1028 4072 Fragments arranged by size: 4072 1028 634 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4763 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3925 3959 1903 Size: 34 1988 34 3678 Fragments arranged by size: 3678 1988 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2047 2571 3626 4836 2047 Size: 524 1055 1210 2945 Fragments arranged by size: 2945 1210 1055 524 BclI T'GATC_A Cuts at: 1548 1548 Size: 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4345 136 Size: 107 122 71 631 605 2673 1525 Fragments arranged by size: 2673 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5729 5729 Size: 5734 BlpI GC'TnA_GC Cuts at: 3151 3151 Size: 5734 Bme1580I G_kGCm'C Cuts at: 1790 2399 2772 3795 4951 1790 Size: 609 373 1023 1156 2573 Fragments arranged by size: 2573 1156 1023 609 373 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5734 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2331 2810 3465 Size: 416 53 83 399 24 914 479 655 Cuts at: 3465 4423 442 Size: 958 1753 Fragments arranged by size: 1753 958 914 655 479 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2081 1056 Size: 1025 4709 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2412 2555 4394 1239 Size: 1173 143 1839 2579 Fragments arranged by size: 2579 1839 1173 143 Bpu10I CC'TnA_GC Cuts at: 2405 2405 Size: 5734 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2657 2864 3859 4724 5022 2657 Size: 207 995 865 298 3369 Fragments arranged by size: 3369 995 865 298 207 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4397 882 Size: 617 2898 2219 Fragments arranged by size: 2898 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3525 493 Size: 711 2321 2702 Fragments arranged by size: 2702 2321 711 BsaBI GATnn'nnATC Cuts at: 5728 5728 Size: 5734 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2656 3541 3923 4558 Size: 53 83 186 936 1123 885 382 635 Cuts at: 4558 275 Size: 1451 Fragments arranged by size: 1451 1123 936 885 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2493 4167 4565 4839 4986 5397 2493 Size: 1674 398 274 147 411 2830 Fragments arranged by size: 2830 1674 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2528 2558 1178 Size: 30 165 30 1125 30 4354 Fragments arranged by size: 4354 1125 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2335 1186 Size: 1149 4585 BseYI C'CCAG_C Cuts at: 2247 2826 2889 4937 5433 2247 Size: 579 63 2048 496 2548 Fragments arranged by size: 2548 2048 579 496 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2730 1163 Size: 573 994 4167 Fragments arranged by size: 4167 994 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3184 3945 4973 1540 Size: 275 186 1183 761 1028 2301 Fragments arranged by size: 2301 1183 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2399 3795 3880 4951 2399 Size: 1396 85 1071 3182 Fragments arranged by size: 3182 1396 1071 85 BsmI GAATG_Cn' Cuts at: 2097 3245 3338 2097 Size: 1148 93 4493 Fragments arranged by size: 4493 1148 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2399 2772 3053 3795 3880 4951 Size: 399 235 374 373 281 742 85 1071 Cuts at: 4951 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 374 373 281 235 85 BspEI T'CCGG_A Cuts at: 5397 5397 Size: 5734 BspHI T'CATG_A Cuts at: 3623 5353 3623 Size: 1730 4004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3089 844 Size: 2245 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3621 1394 Size: 429 1798 3507 Fragments arranged by size: 3507 1798 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4229 4403 65 Size: 1183 2981 174 1396 Fragments arranged by size: 2981 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2037 2486 4378 4565 1700 Size: 110 227 449 1892 187 2869 Fragments arranged by size: 2869 1892 449 227 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5734 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5734 BssSI C'ACGA_G Cuts at: 3794 4806 3794 Size: 1012 4722 BstAPI GCAn_nnn'nTGC Cuts at: 794 2071 794 Size: 1277 4457 BstBI TT'CG_AA Cuts at: 4538 4538 Size: 5734 BstEII G'GTnAC_C Cuts at: 1184 2409 1184 Size: 1225 4509 BstYI r'GATC_y Cuts at: 1972 2115 2148 3064 3431 3816 3833 5285 5371 Size: 143 33 916 367 385 17 1452 86 Cuts at: 5371 5383 5729 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 916 385 367 346 143 86 33 17 12 Bsu36I CC'TnA_GG Cuts at: 2610 2610 Size: 5734 BtgI C'CryG_G Cuts at: 513 1065 2210 2578 513 Size: 552 1145 368 3669 Fragments arranged by size: 3669 1145 552 368 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4995 Fragments arranged by size: 4995 683 56 BtsI GCAGTG_nn' Cuts at: 2770 3250 4044 4064 2770 Size: 480 794 20 4440 Fragments arranged by size: 4440 794 480 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5699 DraI TTT'AAA Cuts at: 3038 3038 Size: 5734 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4741 809 Size: 507 3425 1802 Fragments arranged by size: 3425 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 2488 2970 Size: 54 1425 50 275 18 205 453 482 Cuts at: 2970 3181 4070 5699 8 Size: 211 889 1629 43 Fragments arranged by size: 1629 1425 889 482 453 275 211 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3181 1537 Size: 275 1369 4090 Fragments arranged by size: 4090 1369 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2638 3664 1353 Size: 1285 1026 3423 Fragments arranged by size: 3423 1285 1026 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4321 4695 4841 387 Size: 1064 138 2732 374 146 1280 Fragments arranged by size: 2732 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2328 2412 2555 3797 4394 5181 Size: 393 641 55 84 143 1242 597 787 Cuts at: 5181 5449 1239 Size: 268 1524 Fragments arranged by size: 1524 1242 787 641 597 393 268 143 84 55 EcoO109I rG'GnC_Cy Cuts at: 2804 2992 2804 Size: 188 5546 EcoRI G'AATT_C Cuts at: 2914 3043 3421 2914 Size: 129 378 5227 Fragments arranged by size: 5227 378 129 FseI GG_CCGG'CC Cuts at: 2041 2041 Size: 5734 FspI TGC'GCA Cuts at: 4240 4240 Size: 5734 HaeII r_GCGC'y Cuts at: 1678 4881 1678 Size: 3203 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4375 4407 4449 4481 1373 Size: 32 135 32 2803 32 42 32 2626 Fragments arranged by size: 2803 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3078 3324 669 Size: 988 281 1140 246 3079 Fragments arranged by size: 3079 1140 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5734 HpaI GTT'AAC Cuts at: 3324 3324 Size: 5734 KpnI G_GTAC'C Cuts at: 2475 3059 2475 Size: 584 5150 MfeI C'AATT_G Cuts at: 3333 3333 Size: 5734 MluI A'CGCG_T Cuts at: 5610 5610 Size: 5734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2314 2661 4848 5032 1305 Size: 1009 347 2187 184 2007 Fragments arranged by size: 2187 2007 1009 347 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2972 10 Size: 54 1425 343 1140 2772 Fragments arranged by size: 2772 1425 1140 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3692 4051 4210 518 Size: 648 2526 359 159 2042 Fragments arranged by size: 2526 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2251 2417 2432 2689 2821 3150 3827 4975 Size: 645 166 15 257 132 329 677 1148 Cuts at: 4975 5220 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 645 329 257 245 166 132 15 NaeI GCC'GGC Cuts at: 2039 2039 Size: 5734 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5182 NdeI CA'TA_TG Cuts at: 387 387 Size: 5734 NgoMIV G'CCGG_C Cuts at: 2037 2037 Size: 5734 NheI G'CTAG_C Cuts at: 1052 2077 1052 Size: 1025 4709 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 3092 4637 1132 Size: 97 1863 1545 2229 Fragments arranged by size: 2229 1863 1545 97 PasI CC'CwG_GG Cuts at: 1348 2480 2704 1348 Size: 1132 224 4378 Fragments arranged by size: 4378 1132 224 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5734 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2528 2560 3803 3835 5316 5348 2528 Size: 32 1243 32 1481 32 2914 Fragments arranged by size: 2914 1481 1243 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2804 2804 Size: 5734 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5734 PsiI TTA'TAA Cuts at: 3304 3304 Size: 5734 PstI C_TGCA'G Cuts at: 830 3086 830 Size: 2256 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 PvuII CAG'CTG Cuts at: 2251 2417 2432 2689 2821 2251 Size: 166 15 257 132 5164 Fragments arranged by size: 5164 257 166 132 15 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SanDI GG'GwC_CC Cuts at: 2804 2804 Size: 5734 SapI GCTCTTCn'nnn_ Cuts at: 2638 2638 Size: 5734 SbfI CC_TGCA'GG Cuts at: 3086 3086 Size: 5734 ScaI AGT'ACT Cuts at: 3982 5694 3982 Size: 1712 4022 SfcI C'TryA_G Cuts at: 826 1045 3082 4217 4898 5089 826 Size: 219 2037 1135 681 191 1471 Fragments arranged by size: 2037 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5734 SmaI CCC'GGG Cuts at: 1872 2468 3061 1872 Size: 596 593 4545 Fragments arranged by size: 4545 596 593 SmlI C'TyrA_G Cuts at: 820 1017 1943 2120 2672 2879 3838 4739 5001 Size: 197 926 177 552 207 959 901 262 Cuts at: 5001 820 Size: 1553 Fragments arranged by size: 1553 959 926 901 552 262 207 197 177 SnaBI TAC'GTA Cuts at: 493 3525 493 Size: 3032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 3092 1132 Size: 1960 3774 SspI AAT'ATT Cuts at: 5 52 3658 5 Size: 47 3606 2081 Fragments arranged by size: 3606 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5734 StyI C'CwwG_G Cuts at: 513 1065 2152 2720 3173 513 Size: 552 1087 568 453 3074 Fragments arranged by size: 3074 1087 568 552 453 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2336 2493 3761 3778 3931 4116 4561 Size: 1255 756 157 1268 17 153 185 445 Cuts at: 4561 325 Size: 1498 Fragments arranged by size: 1498 1268 1255 756 445 185 157 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3980 5692 96 Size: 274 80 33 51 1443 2003 1712 138 Fragments arranged by size: 2003 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5694 5726 5694 Size: 32 5702 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5734 XbaI T'CTAG_A Cuts at: 3070 3070 Size: 5734 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5734 XmaI C'CCGG_G Cuts at: 1870 2466 3059 1870 Size: 596 593 4545 Fragments arranged by size: 4545 596 593 XmnI GAAnn'nnTTC Cuts at: 2543 3863 2543 Size: 1320 4414 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3542 276 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI