(Circular) (Six-base) MAPSORT of: pFN21AE0916.seq Check: 4105 from: 1 to: 5566 pFN21AE0916 5566 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3376 278 Size: 53 83 186 936 1840 2468 Fragments arranged by size: 2468 1840 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2331 2909 1937 Size: 394 578 4594 Fragments arranged by size: 4594 578 394 Acc65I G'GTAC_C Cuts at: 2887 2887 Size: 5566 AclI AA'CG_TT Cuts at: 1563 3693 4066 1563 Size: 2130 373 3063 Fragments arranged by size: 3063 2130 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3629 5013 5281 1632 Size: 1997 1384 268 1917 Fragments arranged by size: 1997 1917 1384 268 AfeI AGC'GCT Cuts at: 2390 2390 Size: 5566 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5369 AflIII A'CryG_T Cuts at: 1203 4465 5442 1203 Size: 3262 977 1327 Fragments arranged by size: 3262 1327 977 AgeI A'CCGG_T Cuts at: 4397 4397 Size: 5566 AhdI GACnn_n'nnGTC Cuts at: 4295 4295 Size: 5566 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5566 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5566 AlwNI CAG_nnn'CTG Cuts at: 2530 3309 4881 2530 Size: 779 1572 3215 Fragments arranged by size: 3215 1572 779 ApaI G_GGCC'C Cuts at: 2495 2495 Size: 5566 ApaLI G'TGCA_C Cuts at: 3623 4779 3623 Size: 1156 4410 ApoI r'AATT_y Cuts at: 1493 2875 3101 3253 5362 1493 Size: 1382 226 152 2109 1697 Fragments arranged by size: 2109 1697 1382 226 152 AseI AT'TA_AT Cuts at: 160 1032 4120 160 Size: 872 3088 1606 Fragments arranged by size: 3088 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5566 AvaI C'yCGr_G Cuts at: 1870 1943 2519 2638 2722 2891 1870 Size: 73 576 119 84 169 4545 Fragments arranged by size: 4545 576 169 119 84 73 BamHI G'GATC_C Cuts at: 2896 3263 2896 Size: 367 5199 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2887 4342 618 Size: 325 217 627 1100 1455 1842 Fragments arranged by size: 1842 1455 1100 627 325 217 BanII G_rGCy'C Cuts at: 1391 2478 2495 2885 1391 Size: 1087 17 390 4072 Fragments arranged by size: 4072 1087 390 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4595 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3757 3791 1903 Size: 34 50 34 1736 34 3678 Fragments arranged by size: 3678 1736 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3458 4668 3458 Size: 1210 4356 BclI T'GATC_A Cuts at: 1548 2175 1548 Size: 627 4939 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4177 136 Size: 107 122 71 631 605 2505 1525 Fragments arranged by size: 2505 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5561 5561 Size: 5566 BlpI GC'TnA_GC Cuts at: 2189 2983 2189 Size: 794 4772 Bme1580I G_kGCm'C Cuts at: 1790 2495 3627 4783 1790 Size: 705 1132 1156 2573 Fragments arranged by size: 2573 1156 1132 705 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5566 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3297 4255 442 Size: 416 53 83 399 24 1880 958 1753 Fragments arranged by size: 1880 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5566 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2489 2792 2848 4226 1239 Size: 902 348 303 56 1378 2579 Fragments arranged by size: 2579 1378 902 348 303 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3691 4556 4854 3691 Size: 865 298 4403 Fragments arranged by size: 4403 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4229 882 Size: 617 2730 2219 Fragments arranged by size: 2730 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2073 2181 2655 3357 493 Size: 711 869 108 474 702 2702 Fragments arranged by size: 2702 869 711 702 474 108 BsaBI GATnn'nnATC Cuts at: 2138 5560 2138 Size: 3422 2144 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3373 3755 4390 275 Size: 53 83 186 936 1840 382 635 1451 Fragments arranged by size: 1840 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3999 4397 4671 4818 5229 3999 Size: 398 274 147 411 4336 Fragments arranged by size: 4336 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2108 2138 1178 Size: 30 165 30 705 30 4606 Fragments arranged by size: 4606 705 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2260 2486 1186 Size: 1074 226 4266 Fragments arranged by size: 4266 1074 226 BseYI C'CCAG_C Cuts at: 2379 2608 2627 4769 5265 2379 Size: 229 19 2142 496 2680 Fragments arranged by size: 2680 2142 496 229 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2053 2193 2482 1163 Size: 573 317 140 289 4247 Fragments arranged by size: 4247 573 317 289 140 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2356 3016 3777 4805 1540 Size: 275 186 355 660 761 1028 2301 Fragments arranged by size: 2301 1028 761 660 355 275 186 BsiHKAI G_wGCw'C Cuts at: 2297 3627 3712 4783 2297 Size: 1330 85 1071 3080 Fragments arranged by size: 3080 1330 1071 85 BsmI GAATG_Cn' Cuts at: 3077 3170 3077 Size: 93 5473 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2297 2478 2495 2885 3627 3712 4783 Size: 399 507 181 17 390 742 85 1071 Cuts at: 4783 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 507 399 390 181 85 17 BspEI T'CCGG_A Cuts at: 5229 5229 Size: 5566 BspHI T'CATG_A Cuts at: 3455 5185 3455 Size: 1730 3836 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2317 2430 2448 2921 844 Size: 1473 113 18 473 3489 Fragments arranged by size: 3489 1473 473 113 18 BsrBI CCG'CTC Cuts at: 1394 1823 3453 1394 Size: 429 1630 3507 Fragments arranged by size: 3507 1630 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4061 4235 65 Size: 1183 2813 174 1396 Fragments arranged by size: 2813 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2226 4210 4397 1700 Size: 110 416 1984 187 2869 Fragments arranged by size: 2869 1984 416 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5566 BssHII G'CGCG_C Cuts at: 1925 2554 1925 Size: 629 4937 BssSI C'ACGA_G Cuts at: 2429 3626 4638 2429 Size: 1197 1012 3357 Fragments arranged by size: 3357 1197 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5566 BstBI TT'CG_AA Cuts at: 4370 4370 Size: 5566 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5566 BstYI r'GATC_y Cuts at: 1972 2896 3263 3648 3665 5117 5203 5215 5561 Size: 924 367 385 17 1452 86 12 346 Cuts at: 5561 1972 Size: 1977 Fragments arranged by size: 1977 1452 924 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5014 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2785 487 Size: 683 56 1559 3268 Fragments arranged by size: 3268 1559 683 56 BtsI GCAGTG_nn' Cuts at: 2758 3082 3876 3896 2758 Size: 324 794 20 4428 Fragments arranged by size: 4428 794 324 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5566 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5531 DraI TTT'AAA Cuts at: 2870 2870 Size: 5566 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4573 809 Size: 507 3257 1802 Fragments arranged by size: 3257 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2361 2713 3013 Size: 54 1425 50 275 18 531 352 300 Cuts at: 3013 3902 5531 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 531 352 300 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3013 1537 Size: 275 1201 4090 Fragments arranged by size: 4090 1201 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3496 1353 Size: 2143 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2257 4153 4527 4673 387 Size: 1064 138 668 1896 374 146 1280 Fragments arranged by size: 1896 1280 1064 668 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2489 2792 2848 3629 4226 5013 Size: 393 509 348 303 56 781 597 787 Cuts at: 5013 5281 1239 Size: 268 1524 Fragments arranged by size: 1524 787 781 597 509 393 348 303 268 56 EcoO109I rG'GnC_Cy Cuts at: 2491 2492 2515 2727 2778 2491 Size: 1 23 212 51 5279 Fragments arranged by size: 5279 212 51 23 1 EcoRI G'AATT_C Cuts at: 2875 3253 2875 Size: 378 5188 FspI TGC'GCA Cuts at: 4072 4072 Size: 5566 HaeII r_GCGC'y Cuts at: 1678 2392 2652 4713 1678 Size: 714 260 2061 2531 Fragments arranged by size: 2531 2061 714 260 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2246 2278 2847 2879 4207 Size: 32 135 32 674 32 569 32 1328 Cuts at: 4207 4239 4281 4313 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1328 674 569 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2026 2910 3156 669 Size: 988 281 88 884 246 3079 Fragments arranged by size: 3079 988 884 281 246 88 HindIII A'AGCT_T Cuts at: 748 748 Size: 5566 HpaI GTT'AAC Cuts at: 3156 3156 Size: 5566 KpnI G_GTAC'C Cuts at: 2891 2891 Size: 5566 MfeI C'AATT_G Cuts at: 3165 3165 Size: 5566 MluI A'CGCG_T Cuts at: 5442 5442 Size: 5566 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4680 4864 1305 Size: 3375 184 2007 Fragments arranged by size: 3375 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3744 Fragments arranged by size: 3744 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 2147 3524 3883 4042 518 Size: 648 978 3 1377 359 159 2042 Fragments arranged by size: 2042 1377 978 648 359 159 3 MspA1I CmG'CkG Cuts at: 1606 2383 2530 2667 2982 3659 4807 5052 1606 Size: 777 147 137 315 677 1148 245 2120 Fragments arranged by size: 2120 1148 777 677 315 245 147 137 NaeI GCC'GGC Cuts at: 2228 2228 Size: 5566 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5014 NdeI CA'TA_TG Cuts at: 387 387 Size: 5566 NgoMIV G'CCGG_C Cuts at: 2226 2226 Size: 5566 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5566 NotI GC'GGCC_GC Cuts at: 3013 3013 Size: 5566 NspI r_CATG'y Cuts at: 1132 1229 2924 4469 1132 Size: 97 1695 1545 2229 Fragments arranged by size: 2229 1695 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5566 PciI A'CATG_T Cuts at: 4465 4465 Size: 5566 PflMI CCAn_nnn'nTGG Cuts at: 1108 2104 1108 Size: 996 4570 PmeI GTTT'AAAC Cuts at: 2870 2870 Size: 5566 PmlI CAC'GTG Cuts at: 2073 2181 2655 2073 Size: 108 474 4984 Fragments arranged by size: 4984 474 108 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3635 3667 5148 5180 3635 Size: 32 1481 32 4021 Fragments arranged by size: 4021 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2515 2727 2778 2515 Size: 212 51 5303 Fragments arranged by size: 5303 212 51 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5566 PsiI TTA'TAA Cuts at: 3136 3136 Size: 5566 PspOMI G'GGCC_C Cuts at: 2491 2491 Size: 5566 PstI C_TGCA'G Cuts at: 830 2427 2918 830 Size: 1597 491 3478 Fragments arranged by size: 3478 1597 491 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5566 PvuII CAG'CTG Cuts at: 2530 2530 Size: 5566 SalI G'TCGA_C Cuts at: 1936 2908 1936 Size: 972 4594 SanDI GG'GwC_CC Cuts at: 2515 2778 2515 Size: 263 5303 SbfI CC_TGCA'GG Cuts at: 2918 2918 Size: 5566 ScaI AGT'ACT Cuts at: 3814 5526 3814 Size: 1712 3854 SfcI C'TryA_G Cuts at: 826 1045 2423 2698 2914 4049 4730 4921 826 Size: 219 1378 275 216 1135 681 191 1471 Fragments arranged by size: 1471 1378 1135 681 275 219 216 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5566 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5566 SmaI CCC'GGG Cuts at: 1872 2724 2893 1872 Size: 852 169 4545 Fragments arranged by size: 4545 852 169 SmlI C'TyrA_G Cuts at: 820 1017 1943 3670 4571 4833 820 Size: 197 926 1727 901 262 1553 Fragments arranged by size: 1727 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3357 493 Size: 2864 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5566 SphI G_CATG'C Cuts at: 1132 2924 1132 Size: 1792 3774 SspI AAT'ATT Cuts at: 5 52 3490 5 Size: 47 3438 2081 Fragments arranged by size: 3438 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5566 StyI C'CwwG_G Cuts at: 513 1065 3005 513 Size: 552 1940 3074 Fragments arranged by size: 3074 1940 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3593 3610 3763 3948 4393 325 Size: 1255 2013 17 153 185 445 1498 Fragments arranged by size: 2013 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3812 5524 96 Size: 274 80 33 51 1443 1835 1712 138 Fragments arranged by size: 1835 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5526 5558 5526 Size: 32 5534 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5566 XbaI T'CTAG_A Cuts at: 2902 2902 Size: 5566 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2356 2750 2356 Size: 394 5172 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5566 XmaI C'CCGG_G Cuts at: 1870 2722 2891 1870 Size: 852 169 4545 Fragments arranged by size: 4545 852 169 XmnI GAAnn'nnTTC Cuts at: 3695 3695 Size: 5566 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3374 276 Size: 53 83 186 936 1840 2468 Fragments arranged by size: 2468 1840 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI