(Circular) (Six-base) MAPSORT of: pFN21AB7622.seq Check: 5265 from: 1 to: 5809 pFN21AB7622 5809 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3619 278 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2437 3152 1937 Size: 500 715 4594 Fragments arranged by size: 4594 715 500 Acc65I G'GTAC_C Cuts at: 3130 3130 Size: 5809 AclI AA'CG_TT Cuts at: 1563 3936 4309 1563 Size: 2373 373 3063 Fragments arranged by size: 3063 2373 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2610 2767 3872 5256 5524 1632 Size: 978 157 1105 1384 268 1917 Fragments arranged by size: 1917 1384 1105 978 268 157 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5612 AflIII A'CryG_T Cuts at: 1203 4708 5685 1203 Size: 3505 977 1327 Fragments arranged by size: 3505 1327 977 AgeI A'CCGG_T Cuts at: 2422 4640 2422 Size: 2218 3591 AhdI GACnn_n'nnGTC Cuts at: 4538 4538 Size: 5809 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5809 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2322 1184 Size: 1138 4671 AlwNI CAG_nnn'CTG Cuts at: 3552 5124 3552 Size: 1572 4237 ApaI G_GGCC'C Cuts at: 2202 2202 Size: 5809 ApaLI G'TGCA_C Cuts at: 3866 5022 3866 Size: 1156 4653 ApoI r'AATT_y Cuts at: 1493 2070 2679 2817 2959 3118 3344 3496 5605 Size: 577 609 138 142 159 226 152 2109 Cuts at: 5605 1493 Size: 1697 Fragments arranged by size: 2109 1697 609 577 226 159 152 142 138 AseI AT'TA_AT Cuts at: 160 1032 2973 4363 160 Size: 872 1941 1390 1606 Fragments arranged by size: 1941 1606 1390 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 AvaI C'yCGr_G Cuts at: 1870 1943 3134 1870 Size: 73 1191 4545 Fragments arranged by size: 4545 1191 73 BamHI G'GATC_C Cuts at: 2095 3139 3506 2095 Size: 1044 367 4398 Fragments arranged by size: 4398 1044 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3130 4585 618 Size: 325 217 627 1343 1455 1842 Fragments arranged by size: 1842 1455 1343 627 325 217 BanII G_rGCy'C Cuts at: 1391 2202 2553 2795 3128 1391 Size: 811 351 242 333 4072 Fragments arranged by size: 4072 811 351 333 242 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2469 3076 928 Size: 971 570 607 3661 Fragments arranged by size: 3661 971 607 570 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2214 2248 4000 4034 1903 Size: 34 277 34 1752 34 3678 Fragments arranged by size: 3678 1752 277 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2132 2174 3701 4911 2132 Size: 42 1527 1210 3030 Fragments arranged by size: 3030 1527 1210 42 BclI T'GATC_A Cuts at: 1548 1548 Size: 5809 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4420 136 Size: 107 122 71 631 605 2748 1525 Fragments arranged by size: 2748 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5804 5804 Size: 5809 BlpI GC'TnA_GC Cuts at: 2794 3226 2794 Size: 432 5377 Bme1580I G_kGCm'C Cuts at: 1790 2202 3870 5026 1790 Size: 412 1668 1156 2573 Fragments arranged by size: 2573 1668 1156 412 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5809 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3003 3540 4498 Size: 416 53 83 399 24 1586 537 958 Cuts at: 4498 442 Size: 1753 Fragments arranged by size: 1753 1586 958 537 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5809 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2037 2315 2405 2931 4469 1239 Size: 798 278 90 526 1538 2579 Fragments arranged by size: 2579 1538 798 526 278 90 Bpu10I CC'TnA_GC Cuts at: 2246 2487 2246 Size: 241 5568 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2460 2516 3934 4799 5097 2460 Size: 56 1418 865 298 3172 Fragments arranged by size: 3172 1418 865 298 56 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4472 882 Size: 617 2973 2219 Fragments arranged by size: 2973 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3600 493 Size: 711 2396 2702 Fragments arranged by size: 2702 2396 711 BsaBI GATnn'nnATC Cuts at: 2349 5803 2349 Size: 3454 2355 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3616 3998 4633 275 Size: 53 83 186 936 2083 382 635 1451 Fragments arranged by size: 2083 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2422 4242 4640 4914 5061 5472 2422 Size: 1820 398 274 147 411 2759 Fragments arranged by size: 2759 1820 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5584 Fragments arranged by size: 5584 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2381 1186 Size: 919 276 4614 Fragments arranged by size: 4614 919 276 BseYI C'CCAG_C Cuts at: 5012 5508 5012 Size: 496 5313 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1995 1163 Size: 573 259 4977 Fragments arranged by size: 4977 573 259 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3259 4020 5048 1540 Size: 275 186 1258 761 1028 2301 Fragments arranged by size: 2301 1258 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2553 2602 3870 3955 5026 2553 Size: 49 1268 85 1071 3336 Fragments arranged by size: 3336 1268 1071 85 49 BsmI GAATG_Cn' Cuts at: 2864 3017 3320 3413 2864 Size: 153 303 93 5260 Fragments arranged by size: 5260 303 153 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2249 2249 Size: 5809 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2553 2602 2795 3128 3870 3955 Size: 399 412 351 49 193 333 742 85 Cuts at: 3955 5026 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 412 399 351 333 193 85 49 BspEI T'CCGG_A Cuts at: 5472 5472 Size: 5809 BspHI T'CATG_A Cuts at: 3698 5428 3698 Size: 1730 4079 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2706 3164 844 Size: 1862 458 3489 Fragments arranged by size: 3489 1862 458 BsrBI CCG'CTC Cuts at: 1394 1823 3696 1394 Size: 429 1873 3507 Fragments arranged by size: 3507 1873 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4304 4478 65 Size: 1183 3056 174 1396 Fragments arranged by size: 3056 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2422 4453 4640 1700 Size: 110 612 2031 187 2869 Fragments arranged by size: 2869 2031 612 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5809 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5809 BssSI C'ACGA_G Cuts at: 3869 4881 3869 Size: 1012 4797 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5809 BstBI TT'CG_AA Cuts at: 4613 4613 Size: 5809 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5809 BstYI r'GATC_y Cuts at: 1972 2095 3139 3506 3891 3908 5360 5446 5458 Size: 123 1044 367 385 17 1452 86 12 Cuts at: 5458 5804 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1044 385 367 346 123 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2561 2561 Size: 5809 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5257 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5070 Fragments arranged by size: 5070 683 56 BtsI GCAGTG_nn' Cuts at: 2379 3325 4119 4139 2379 Size: 946 794 20 4049 Fragments arranged by size: 4049 946 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5809 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5774 DraI TTT'AAA Cuts at: 2686 3113 2686 Size: 427 5382 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4816 809 Size: 507 3500 1802 Fragments arranged by size: 3500 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3256 4145 5774 Size: 54 1425 50 275 18 1426 889 1629 Cuts at: 5774 8 Size: 43 Fragments arranged by size: 1629 1426 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3256 1537 Size: 275 1444 4090 Fragments arranged by size: 4090 1444 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2055 2103 3739 1353 Size: 702 48 1636 3423 Fragments arranged by size: 3423 1636 702 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4396 4770 4916 387 Size: 1064 138 2807 374 146 1280 Fragments arranged by size: 2807 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2551 2551 Size: 5809 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2315 2405 2610 2767 2931 3872 Size: 393 405 278 90 205 157 164 941 Cuts at: 3872 4469 5256 5524 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 941 787 597 405 393 278 268 205 164 157 90 EcoO109I rG'GnC_Cy Cuts at: 2199 2199 Size: 5809 EcoRI G'AATT_C Cuts at: 2817 2959 3118 3496 2817 Size: 142 159 378 5130 Fragments arranged by size: 5130 378 159 142 FspI TGC'GCA Cuts at: 4315 4315 Size: 5809 HaeII r_GCGC'y Cuts at: 1678 4956 1678 Size: 3278 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2106 2138 2456 2488 4450 Size: 32 135 32 534 32 318 32 1962 Cuts at: 4450 4482 4524 4556 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1962 534 318 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2438 3153 3399 669 Size: 988 281 500 715 246 3079 Fragments arranged by size: 3079 988 715 500 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5809 HpaI GTT'AAC Cuts at: 3399 3399 Size: 5809 KpnI G_GTAC'C Cuts at: 3134 3134 Size: 5809 MfeI C'AATT_G Cuts at: 3408 3408 Size: 5809 MluI A'CGCG_T Cuts at: 5685 5685 Size: 5809 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2067 2182 4923 5107 1305 Size: 762 115 2741 184 2007 Fragments arranged by size: 2741 2007 762 184 115 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3987 Fragments arranged by size: 3987 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2571 2760 2885 3767 4126 4285 518 Size: 648 1405 189 125 882 359 159 2042 Fragments arranged by size: 2042 1405 882 648 359 189 159 125 MspA1I CmG'CkG Cuts at: 1606 3225 3902 5050 5295 1606 Size: 1619 677 1148 245 2120 Fragments arranged by size: 2120 1619 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5257 NdeI CA'TA_TG Cuts at: 387 387 Size: 5809 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5809 NotI GC'GGCC_GC Cuts at: 3256 3256 Size: 5809 NspI r_CATG'y Cuts at: 1132 1229 3167 4712 1132 Size: 97 1938 1545 2229 Fragments arranged by size: 2229 1938 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5809 PciI A'CATG_T Cuts at: 4708 4708 Size: 5809 PflMI CCAn_nnn'nTGG Cuts at: 1108 2360 1108 Size: 1252 4557 PmeI GTTT'AAAC Cuts at: 3113 3113 Size: 5809 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3878 3910 5391 5423 3878 Size: 32 1481 32 4264 Fragments arranged by size: 4264 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5809 PsiI TTA'TAA Cuts at: 3379 3379 Size: 5809 PspOMI G'GGCC_C Cuts at: 2198 2198 Size: 5809 PstI C_TGCA'G Cuts at: 830 3161 830 Size: 2331 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5809 SacI G_AGCT'C Cuts at: 2553 2553 Size: 5809 SalI G'TCGA_C Cuts at: 1936 2436 3151 1936 Size: 500 715 4594 Fragments arranged by size: 4594 715 500 SbfI CC_TGCA'GG Cuts at: 3161 3161 Size: 5809 ScaI AGT'ACT Cuts at: 2454 4057 5769 2454 Size: 1603 1712 2494 Fragments arranged by size: 2494 1712 1603 SfcI C'TryA_G Cuts at: 826 1045 2327 3157 4292 4973 5164 826 Size: 219 1282 830 1135 681 191 1471 Fragments arranged by size: 1471 1282 1135 830 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5809 SmaI CCC'GGG Cuts at: 1872 3136 1872 Size: 1264 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2475 2531 3913 4814 5076 820 Size: 197 926 532 56 1382 901 262 1553 Fragments arranged by size: 1553 1382 926 901 532 262 197 56 SnaBI TAC'GTA Cuts at: 493 3600 493 Size: 3107 2702 SpeI A'CTAG_T Cuts at: 152 2455 152 Size: 2303 3506 SphI G_CATG'C Cuts at: 1132 3167 1132 Size: 2035 3774 SspI AAT'ATT Cuts at: 5 52 3733 5 Size: 47 3681 2081 Fragments arranged by size: 3681 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5809 StyI C'CwwG_G Cuts at: 513 1065 2709 3248 513 Size: 552 1644 539 3074 Fragments arranged by size: 3074 1644 552 539 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2657 3836 3853 4006 4191 4636 325 Size: 1255 1077 1179 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1179 1077 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2452 4055 5767 Size: 274 80 33 51 1443 475 1603 1712 Cuts at: 5767 96 Size: 138 Fragments arranged by size: 1712 1603 1443 475 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5769 5801 5769 Size: 32 5777 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5809 XbaI T'CTAG_A Cuts at: 3145 3145 Size: 5809 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2192 2648 2192 Size: 456 5353 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5809 XmaI C'CCGG_G Cuts at: 1870 3134 1870 Size: 1264 4545 XmnI GAAnn'nnTTC Cuts at: 2070 2864 3015 3087 3938 2070 Size: 794 151 72 851 3941 Fragments arranged by size: 3941 851 794 151 72 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3617 276 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI