Restriction map for
pFN21AB6873
##################################
# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MaeIII MamI MfeI
MflI MluI MroI MseI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp45I Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AspHI 85 GAGCTC G[AT]GC[AT]^C
AvaI 202 CCCGAG C^[CT]CG[GA]G
BanI 488 GGCACC G^G[CT][GA]CC
BanII 85 GAGCTC G[GA]GC[CT]^C
BbsI 276 GAAGAC GAAGAC(2/6)
BbvI 333 GCTGC GCAGC(8/12)
BcnI 48 CCCGG CC^[GC]GG
BmyI 85 GAGCTC G[AGT]GC[ACT]^C
BpuAI 276 GAAGAC GAAGAC(2/6)
BsgI 226 CTGCAC GTGCAG(16/14)
BsiHKAI 85 GAGCTC G[AT]GC[AT]^C
BsoBI 202 CCCGAG C^[CT]CG[GA]G
Bsp1286I 85 GAGCTC G[AGT]GC[ACT]^C
BsrI 411 CCAGT ACTGG(1/-1)
BstAPI 401 GCATGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EarI 523 GAAGAG CTCTTC(1/4)
EcoNI 429 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 333 GCTGC GC^[ACGT]GC
HapII 49 CCGG C^CGG
HpaII 49 CCGG C^CGG
ItaI 333 GCTGC GC^[ACGT]GC
Ksp632I 523 GAAGAG CTCTTC(1/4)
MslI 117 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 246 CAGCTG C[AC]G^C[GT]G
MspI 49 CCGG C^CGG
NciI 48 CCCGG CC^[GC]GG
PflMI 411 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 425 GAGTC GAGTC(4/5)
PvuII 246 CAGCTG CAG^CTG
SacI 85 GAGCTC GAGCT^C
SapI 523 GAAGAGC GCTCTTC(1/4)
SfcI 373 CTACAG C^T[GA][CT]AG
TaqI 338 TCGA T^CGA
TseI 333 GCTGC G^C[AT]GC
Tsp509I 355 AATT ^AATT
TthHB8I 338 TCGA T^CGA
Van91I 411 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 414 GTAC GT^AC
498 GTAC
AluI 86 AGCT AG^CT
247 AGCT
AlwNI 76 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
412 CAGTACCTG
BalI 119 TGGCCA TGG^CCA
419 TGGCCA
BpmI 82 CTGGAG CTGGAG(16/14)
214 CTGGAG
BsmFI 103 GGGAC GGGAC(10/14)
46 GTCCC
BstF5I 60 CATCC GGATG(2/0)
393 CATCC
BstXI 116 CCATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
422 CCAGAGTCCTGG
DdeI 169 CTGAG C^T[ACGT]AG
281 CTGAG
FokI 60 CATCC GGATG(9/13)
393 CATCC
HphI 17 TCACC GGTGA(8/7)
182 TCACC
MluNI 119 TGGCCA TGG^CCA
419 TGGCCA
MscI 119 TGGCCA TGG^CCA
419 TGGCCA
MwoI 319 GCGAACGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
401 GCATGATGTGC
RsaI 414 GTAC GT^AC
498 GTAC
TfiI 285 GAATC G^A[AT]TC
458 GATTC
TspRI 155 CACTG CA[GC]TG[ACGT][ACGT]^
188 CACTG
XcmI 71 CCAAGCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
422 CCAGAGTCCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AvaII BsaJI BsiYI BslI BssKI
BstNI Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I HaeIII
HinfI MboI MboII MnlI MvaI
NcoI NdeII NlaIII NlaIV PpuMI
Sau3AI Sau96I ScrFI StyI