Restriction map for
pFN21AB6873
(Linear) (Six-base) MAPSORT of: KIBB6873.seq Check: 3906 from: 1 to: 540
KIBB6873.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 540
Size: 178 362
AlwNI CAG_nnn'CTG
Cuts at: 0 81 417 540
Size: 81 336 123
Fragments arranged by size:
336 123 81
AvaI C'yCGr_G
Cuts at: 0 202 540
Size: 202 338
BanI G'GyrC_C
Cuts at: 0 488 540
Size: 488 52
BanII G_rGCy'C
Cuts at: 0 89 540
Size: 89 451
BbsI GAAGACnn'nnnn_
Cuts at: 0 283 540
Size: 283 257
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 235 540
Size: 103 132 305
Fragments arranged by size:
305 132 103
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 540
Size: 124 30 386
Fragments arranged by size:
386 124 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 540
Size: 211 329
BsiHKAI G_wGCw'C
Cuts at: 0 89 540
Size: 89 451
Bsp1286I G_dGCh'C
Cuts at: 0 89 540
Size: 89 451
BstAPI GCAn_nnn'nTGC
Cuts at: 0 407 540
Size: 407 133
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 429 540
Size: 123 306 111
Fragments arranged by size:
306 123 111
BtgI C'CryG_G
Cuts at: 0 53 116 122 540
Size: 53 63 6 418
Fragments arranged by size:
418 63 53 6
EaeI y'GGCC_r
Cuts at: 0 50 119 419 540
Size: 50 69 300 121
Fragments arranged by size:
300 121 69 50
EarI CTCTTCn'nnn_
Cuts at: 0 518 540
Size: 518 22
EcoICRI GAG'CTC
Cuts at: 0 87 540
Size: 87 453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 178 235 540
Size: 103 75 57 305
Fragments arranged by size:
305 103 75 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 433 540
Size: 433 107
EcoO109I rG'GnC_Cy
Cuts at: 0 78 104 162 264 476 540
Size: 78 26 58 102 212 64
Fragments arranged by size:
212 102 78 64 58 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 270 302 540
Size: 270 32 238
Fragments arranged by size:
270 238 32
MscI TGG'CCA
Cuts at: 0 121 421 540
Size: 121 300 119
Fragments arranged by size:
300 121 119
MslI CAynn'nnrTG
Cuts at: 0 121 540
Size: 121 419
MspA1I CmG'CkG
Cuts at: 0 248 540
Size: 248 292
NcoI C'CATG_G
Cuts at: 0 53 116 122 540
Size: 53 63 6 418
Fragments arranged by size:
418 63 53 6
PasI CC'CwG_GG
Cuts at: 0 21 540
Size: 21 519
PflMI CCAn_nnn'nTGG
Cuts at: 0 417 540
Size: 417 123
PpuMI rG'GwC_Cy
Cuts at: 0 78 104 264 540
Size: 78 26 160 276
Fragments arranged by size:
276 160 78 26
PvuII CAG'CTG
Cuts at: 0 248 540
Size: 248 292
SacI G_AGCT'C
Cuts at: 0 89 540
Size: 89 451
SapI GCTCTTCn'nnn_
Cuts at: 0 518 540
Size: 518 22
SfcI C'TryA_G
Cuts at: 0 373 540
Size: 373 167
StyI C'CwwG_G
Cuts at: 0 53 116 122 267 540
Size: 53 63 6 145 273
Fragments arranged by size:
273 145 63 53 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 514 540
Size: 514 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 78 429 540
Size: 78 351 111
Fragments arranged by size:
351 111 78
Enzymes that do cut:
AcuI AlwNI AvaI BanI BanII BbsI BpmI BsaXI
BsgI BsiHKAI Bsp1286I BstAPI BstXI BtgI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I Hin4I MscI MslI MspA1I
NcoI PasI PflMI PpuMI PvuII SacI SapI SfcI
StyI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI
ZraI