(Linear) (Six-base) MAPSORT of: KIEE2173.seq Check: 6645 from: 1 to: 2193 KIEE2173.orf With 182 enzymes: SgfI * March 22, 2012 15:57 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 893 1033 1102 1633 2193 Size: 893 140 69 531 560 Fragments arranged by size: 893 560 531 140 69 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1969 2193 Size: 1969 224 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1249 1281 2193 Size: 1249 32 912 Fragments arranged by size: 1249 912 32 AlwNI CAG_nnn'CTG Cuts at: 0 199 352 699 942 1452 1548 2193 Size: 199 153 347 243 510 96 645 Fragments arranged by size: 645 510 347 243 199 153 96 ApoI r'AATT_y Cuts at: 0 519 1161 1697 2063 2193 Size: 519 642 536 366 130 Fragments arranged by size: 642 536 519 366 130 AseI AT'TA_AT Cuts at: 0 1282 1916 2193 Size: 1282 634 277 Fragments arranged by size: 1282 634 277 AvaI C'yCGr_G Cuts at: 0 839 2193 Size: 839 1354 AvrII C'CTAG_G Cuts at: 0 310 2193 Size: 310 1883 BanII G_rGCy'C Cuts at: 0 457 855 2193 Size: 457 398 1338 Fragments arranged by size: 1338 457 398 BbsI GAAGACnn'nnnn_ Cuts at: 0 1901 2193 Size: 1901 292 BbvCI CC'TCA_GC Cuts at: 0 700 2193 Size: 700 1493 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1108 1142 2193 Size: 1108 34 1051 Fragments arranged by size: 1108 1051 34 BclI T'GATC_A Cuts at: 0 54 2040 2193 Size: 54 1986 153 Fragments arranged by size: 1986 153 54 BfrBI ATG'CAT Cuts at: 0 301 2193 Size: 301 1892 BglII A'GATC_T Cuts at: 0 36 1641 2193 Size: 36 1605 552 Fragments arranged by size: 1605 552 36 BmrI ACTGGGnnnn_n' Cuts at: 0 482 2193 Size: 482 1711 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 706 907 1624 1834 1876 2193 Size: 706 201 717 210 42 317 Fragments arranged by size: 717 706 317 210 201 42 Bpu10I CC'TnA_GC Cuts at: 0 700 2193 Size: 700 1493 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 749 2193 Size: 749 1444 BsaAI yAC'GTr Cuts at: 0 182 2193 Size: 182 2011 BsaBI GATnn'nnATC Cuts at: 0 1646 2193 Size: 1646 547 BseYI C'CCAG_C Cuts at: 0 896 1564 2193 Size: 896 668 629 Fragments arranged by size: 896 668 629 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1112 2193 Size: 1112 1081 BsiHKAI G_wGCw'C Cuts at: 0 457 2193 Size: 457 1736 BsmI GAATG_Cn' Cuts at: 0 813 2193 Size: 813 1380 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1682 2193 Size: 1682 511 Bsp1286I G_dGCh'C Cuts at: 0 457 855 2193 Size: 457 398 1338 Fragments arranged by size: 1338 457 398 BspHI T'CATG_A Cuts at: 0 2135 2193 Size: 2135 58 BsrDI GCAATG_nn' Cuts at: 0 1663 2193 Size: 1663 530 BstBI TT'CG_AA Cuts at: 0 210 2193 Size: 210 1983 BstXI CCAn_nnnn'nTGG Cuts at: 0 1395 1564 2185 2193 Size: 1395 169 621 8 Fragments arranged by size: 1395 621 169 8 BstYI r'GATC_y Cuts at: 0 36 259 751 1477 1545 1641 2193 Size: 36 223 492 726 68 96 552 Fragments arranged by size: 726 552 492 223 96 68 36 BtsI GCAGTG_nn' Cuts at: 0 239 1382 2193 Size: 239 1143 811 Fragments arranged by size: 1143 811 239 DraI TTT'AAA Cuts at: 0 44 722 2193 Size: 44 678 1471 Fragments arranged by size: 1471 678 44 EaeI y'GGCC_r Cuts at: 0 1184 2193 Size: 1184 1009 EarI CTCTTCn'nnn_ Cuts at: 0 1052 1078 1207 2193 Size: 1052 26 129 986 Fragments arranged by size: 1052 986 129 26 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1872 2193 Size: 1872 321 EcoICRI GAG'CTC Cuts at: 0 455 2193 Size: 455 1738 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 706 893 907 1033 1102 1624 1633 1834 Size: 706 187 14 126 69 522 9 201 Cuts at: 1834 1876 2193 Size: 42 317 Fragments arranged by size: 706 522 317 201 187 126 69 42 14 9 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1275 1660 2193 Size: 1275 385 533 Fragments arranged by size: 1275 533 385 HaeII r_GCGC'y Cuts at: 0 1863 2193 Size: 1863 330 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 643 675 2193 Size: 643 32 1518 Fragments arranged by size: 1518 643 32 HincII GTy'rAC Cuts at: 0 2150 2193 Size: 2150 43 MfeI C'AATT_G Cuts at: 0 2036 2193 Size: 2036 157 MspA1I CmG'CkG Cuts at: 0 199 696 900 2193 Size: 199 497 204 1293 Fragments arranged by size: 1293 497 204 199 NdeI CA'TA_TG Cuts at: 0 2184 2193 Size: 2184 9 NsiI A_TGCA'T Cuts at: 0 303 2193 Size: 303 1890 PfoI T'CCnGG_A Cuts at: 0 923 2193 Size: 923 1270 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1870 1902 2193 Size: 1870 32 291 Fragments arranged by size: 1870 291 32 PshAI GACnn'nnGTC Cuts at: 0 442 2193 Size: 442 1751 PspXI vC'TCGA_Gb Cuts at: 0 839 2193 Size: 839 1354 PstI C_TGCA'G Cuts at: 0 1028 1043 1232 2193 Size: 1028 15 189 961 Fragments arranged by size: 1028 961 189 15 PvuII CAG'CTG Cuts at: 0 199 696 900 2193 Size: 199 497 204 1293 Fragments arranged by size: 1293 497 204 199 SacI G_AGCT'C Cuts at: 0 457 2193 Size: 457 1736 ScaI AGT'ACT Cuts at: 0 948 2193 Size: 948 1245 SfcI C'TryA_G Cuts at: 0 1024 1039 1228 2193 Size: 1024 15 189 965 Fragments arranged by size: 1024 965 189 15 SmlI C'TyrA_G Cuts at: 0 764 839 2193 Size: 764 75 1354 Fragments arranged by size: 1354 764 75 SnaBI TAC'GTA Cuts at: 0 182 2193 Size: 182 2011 StuI AGG'CCT Cuts at: 0 280 315 1370 2193 Size: 280 35 1055 823 Fragments arranged by size: 1055 823 280 35 StyI C'CwwG_G Cuts at: 0 310 2193 Size: 310 1883 TatI w'GTAC_w Cuts at: 0 906 946 2193 Size: 906 40 1247 Fragments arranged by size: 1247 906 40 XhoI C'TCGA_G Cuts at: 0 839 2193 Size: 839 1354 XmnI GAAnn'nnTTC Cuts at: 0 1409 1697 2193 Size: 1409 288 496 Fragments arranged by size: 1409 496 288 Enzymes that do cut: AcuI AlfI AloI AlwNI ApoI AseI AvaI AvrII BanII BbsI BbvCI BcgI BclI BfrBI BglII BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BstBI BstXI BstYI BtsI DraI EaeI EarI EciI EcoICRI Eco57MI FalI HaeII Hin4I HincII MfeI MspA1I NdeI NsiI PfoI PpiI PshAI PspXI PstI PvuII SacI ScaI SfcI SmlI SnaBI StuI StyI TatI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI ApaI ApaLI AscI AsiSI BaeI BamHI BanI BbeI BciVI BglI BlpI Bme1580I BmgBI BmtI BplI BsaI BsaHI BsaWI BsaXI BseRI BsiEI BsiWI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EagI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MluI MmeI MscI MslI NaeI NarI NcoI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PmeI PmlI PpuMI PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI XmaI ZraI