(Circular) (Six-base) MAPSORT of: pFN21AB6591.seq Check: 4965 from: 1 to: 5845 pFN21AB6591 5845 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2619 3655 278 Size: 53 83 186 936 1083 1036 2468 Fragments arranged by size: 2468 1083 1036 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2199 2358 3188 1937 Size: 262 159 830 4594 Fragments arranged by size: 4594 830 262 159 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3068 3908 5292 5560 1632 Size: 1436 840 1384 268 1917 Fragments arranged by size: 1917 1436 1384 840 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 2427 4744 5721 1203 Size: 1224 2317 977 1327 Fragments arranged by size: 2317 1327 1224 977 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 4574 4574 Size: 5845 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5845 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2570 1184 Size: 1386 4459 AlwNI CAG_nnn'CTG Cuts at: 2088 3588 5160 2088 Size: 1500 1572 2773 Fragments arranged by size: 2773 1572 1500 ApaI G_GGCC'C Cuts at: 2045 2045 Size: 5845 ApaLI G'TGCA_C Cuts at: 3902 5058 3902 Size: 1156 4689 ApoI r'AATT_y Cuts at: 1493 2368 3154 3380 3532 5641 1493 Size: 875 786 226 152 2109 1697 Fragments arranged by size: 2109 1697 875 786 226 152 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 2056 2790 3170 1870 Size: 73 113 734 380 4545 Fragments arranged by size: 4545 734 380 113 73 BamHI G'GATC_C Cuts at: 3100 3175 3542 3100 Size: 75 367 5403 Fragments arranged by size: 5403 367 75 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2597 3166 4621 618 Size: 325 217 627 810 569 1455 1842 Fragments arranged by size: 1842 1455 810 627 569 325 217 BanII G_rGCy'C Cuts at: 1391 2045 2112 2157 3164 1391 Size: 654 67 45 1007 4072 Fragments arranged by size: 4072 1007 654 67 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2282 2619 2686 2842 928 Size: 971 383 337 67 156 3931 Fragments arranged by size: 3931 971 383 337 156 67 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4036 4070 1903 Size: 34 2099 34 3678 Fragments arranged by size: 3678 2099 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3737 4947 3737 Size: 1210 4635 BclI T'GATC_A Cuts at: 1548 2253 1548 Size: 705 5140 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4456 136 Size: 107 122 71 631 605 2784 1525 Fragments arranged by size: 2784 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2022 2685 5840 2022 Size: 663 3155 2027 Fragments arranged by size: 3155 2027 663 BlpI GC'TnA_GC Cuts at: 3262 3262 Size: 5845 Bme1580I G_kGCm'C Cuts at: 1790 2045 3906 5062 1790 Size: 255 1861 1156 2573 Fragments arranged by size: 2573 1861 1156 255 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3576 4534 442 Size: 416 53 83 399 24 2159 958 1753 Fragments arranged by size: 2159 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2917 4505 1239 Size: 932 746 1588 2579 Fragments arranged by size: 2579 1588 932 746 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2747 3970 4835 5133 2747 Size: 1223 865 298 3459 Fragments arranged by size: 3459 1223 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4508 882 Size: 617 3009 2219 Fragments arranged by size: 3009 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3636 493 Size: 711 2432 2702 Fragments arranged by size: 2702 2432 711 BsaBI GATnn'nnATC Cuts at: 2021 5839 2021 Size: 3818 2027 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2616 3652 4034 4669 Size: 53 83 186 936 1083 1036 382 635 Cuts at: 4669 275 Size: 1451 Fragments arranged by size: 1451 1083 1036 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4278 4676 4950 5097 5508 4278 Size: 398 274 147 411 4615 Fragments arranged by size: 4615 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5620 Fragments arranged by size: 5620 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5845 BseYI C'CCAG_C Cuts at: 2100 5048 5544 2100 Size: 2948 496 2401 Fragments arranged by size: 2948 2401 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3295 4056 5084 1540 Size: 275 186 1294 761 1028 2301 Fragments arranged by size: 2301 1294 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2636 2909 3074 3906 3991 5062 2636 Size: 273 165 832 85 1071 3419 Fragments arranged by size: 3419 1071 832 273 165 85 BsmI GAATG_Cn' Cuts at: 3356 3449 3356 Size: 93 5752 BsmBI CGTCTCn'nnnn_ Cuts at: 2765 2765 Size: 5845 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2112 2157 2636 2909 3074 3164 Size: 399 255 67 45 479 273 165 90 Cuts at: 3164 3906 3991 5062 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 479 399 273 255 165 90 85 67 45 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 2679 3734 5464 2679 Size: 1055 1730 3060 Fragments arranged by size: 3060 1730 1055 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2057 2817 3200 844 Size: 1213 760 383 3489 Fragments arranged by size: 3489 1213 760 383 BsrBI CCG'CTC Cuts at: 1394 1823 3064 3732 1394 Size: 429 1241 668 3507 Fragments arranged by size: 3507 1241 668 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2475 4340 4514 65 Size: 1183 1227 1865 174 1396 Fragments arranged by size: 1865 1396 1227 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2129 4489 4676 1700 Size: 110 319 2360 187 2869 Fragments arranged by size: 2869 2360 319 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5845 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5845 BssSI C'ACGA_G Cuts at: 3905 4917 3905 Size: 1012 4833 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5845 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5845 BstYI r'GATC_y Cuts at: 1972 2022 2685 3100 3175 3542 3927 3944 5396 Size: 50 663 415 75 367 385 17 1452 Cuts at: 5396 5482 5494 5840 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 663 415 385 367 346 86 75 50 17 12 Bsu36I CC'TnA_GG Cuts at: 2956 3113 2956 Size: 157 5688 BtgI C'CryG_G Cuts at: 513 1065 2004 2029 513 Size: 552 939 25 4329 Fragments arranged by size: 4329 939 552 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2935 487 Size: 683 56 1709 3397 Fragments arranged by size: 3397 1709 683 56 BtsI GCAGTG_nn' Cuts at: 3361 4155 4175 3361 Size: 794 20 5031 Fragments arranged by size: 5031 794 20 ClaI AT'CG_AT Cuts at: 1554 3123 1554 Size: 1569 4276 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 3149 3149 Size: 5845 DraIII CAC_nnn'GTG Cuts at: 2494 2494 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2621 4852 809 Size: 507 1305 2231 1802 Fragments arranged by size: 2231 1802 1305 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 3292 4181 Size: 54 1425 50 275 18 342 1120 889 Cuts at: 4181 5810 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1120 889 342 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3292 1537 Size: 275 1480 4090 Fragments arranged by size: 4090 1480 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2681 3775 1353 Size: 1328 1094 3423 Fragments arranged by size: 3423 1328 1094 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4432 4806 4952 387 Size: 1064 138 2843 374 146 1280 Fragments arranged by size: 2843 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2917 3068 3908 4505 5292 5560 Size: 393 539 746 151 840 597 787 268 Cuts at: 5560 1239 Size: 1524 Fragments arranged by size: 1524 840 787 746 597 539 393 268 151 EcoNI CCTnn'n_nnAGG Cuts at: 2891 2891 Size: 5845 EcoO109I rG'GnC_Cy Cuts at: 2042 2671 2042 Size: 629 5216 EcoRI G'AATT_C Cuts at: 2368 3154 3532 2368 Size: 786 378 4681 Fragments arranged by size: 4681 786 378 FspI TGC'GCA Cuts at: 4351 4351 Size: 5845 HaeII r_GCGC'y Cuts at: 1678 2064 2088 2130 4992 1678 Size: 386 24 42 2862 2531 Fragments arranged by size: 2862 2531 386 42 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2645 2677 2743 2775 4486 Size: 32 135 32 1073 32 66 32 1711 Cuts at: 4486 4518 4560 4592 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1711 1073 135 66 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3189 3435 669 Size: 988 281 1251 246 3079 Fragments arranged by size: 3079 1251 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5845 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4959 5143 1305 Size: 3654 184 2007 Fragments arranged by size: 3654 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4023 Fragments arranged by size: 4023 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 2270 2480 2747 2888 3803 4162 Size: 648 1083 21 210 267 141 915 359 Cuts at: 4162 4321 518 Size: 159 2042 Fragments arranged by size: 2042 1083 915 648 359 267 210 159 141 21 MspA1I CmG'CkG Cuts at: 1606 2031 2149 2924 3261 3938 5086 5331 1606 Size: 425 118 775 337 677 1148 245 2120 Fragments arranged by size: 2120 1148 775 677 425 337 245 118 NaeI GCC'GGC Cuts at: 2131 2131 Size: 5845 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4354 Fragments arranged by size: 4354 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NgoMIV G'CCGG_C Cuts at: 2129 2129 Size: 5845 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 2431 3203 4748 1132 Size: 97 1202 772 1545 2229 Fragments arranged by size: 2229 1545 1202 772 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5845 PciI A'CATG_T Cuts at: 2427 4744 2427 Size: 2317 3528 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5845 PfoI T'CCnGG_A Cuts at: 3027 3027 Size: 5845 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3946 5427 5459 3914 Size: 32 1481 32 4300 Fragments arranged by size: 4300 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2671 2671 Size: 5845 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5845 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PspOMI G'GGCC_C Cuts at: 2041 2041 Size: 5845 PstI C_TGCA'G Cuts at: 830 3197 830 Size: 2367 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 PvuII CAG'CTG Cuts at: 2149 2924 2149 Size: 775 5070 SacII CC_GC'GG Cuts at: 2032 2032 Size: 5845 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SfcI C'TryA_G Cuts at: 826 1045 3193 4328 5009 5200 826 Size: 219 2148 1135 681 191 1471 Fragments arranged by size: 2148 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 3172 1872 Size: 1300 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2762 3949 4850 5112 820 Size: 197 926 819 1187 901 262 1553 Fragments arranged by size: 1553 1187 926 901 819 262 197 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 3769 5 Size: 47 3717 2081 Fragments arranged by size: 3717 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5845 StyI C'CwwG_G Cuts at: 513 1065 2004 2590 3284 513 Size: 552 939 586 694 3074 Fragments arranged by size: 3074 939 694 586 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3872 3889 4042 4227 4672 325 Size: 1255 2292 17 153 185 445 1498 Fragments arranged by size: 2292 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4091 5803 96 Size: 274 80 33 51 1443 2114 1712 138 Fragments arranged by size: 2114 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2378 2410 5805 5837 2378 Size: 32 3395 32 2386 Fragments arranged by size: 3395 2386 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5845 XbaI T'CTAG_A Cuts at: 3181 3181 Size: 5845 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2461 2830 2461 Size: 369 5476 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 3170 1870 Size: 1300 4545 XmnI GAAnn'nnTTC Cuts at: 3974 3974 Size: 5845 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2617 3653 276 Size: 53 83 186 936 1083 1036 2468 Fragments arranged by size: 2468 1083 1036 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI