Restriction map for
pFN21AB6343
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HaeII HaeIII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HphI
KasI KpnI Ksp632I KspI MaeII
MfeI MluI MluNI MscI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 477 TCCGGA T^CCGGA
AocI 706 CCTAAGG CC^T[ACGT]AGG
AspHI 233 GAGCAC G[AT]GC[AT]^C
BanI 160 GGTGCC G^G[CT][GA]CC
BbsI 784 GAAGAC GAAGAC(2/6)
BbvI 303 GCAGC GCAGC(8/12)
BfaI 230 CTAG C^TAG
BglII 190 AGATCT A^GATCT
BmyI 233 GAGCAC G[AGT]GC[ACT]^C
BpmI 371 CTCCAG CTGGAG(16/14)
BpuAI 784 GAAGAC GAAGAC(2/6)
BsaBI 713 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 477 TCCGGA [AT]^CCGG[AT]
BseAI 477 TCCGGA T^CCGGA
BseRI 320 CTCCTC GAGGAG(10/8)
BsiHKAI 233 GAGCAC G[AT]GC[AT]^C
BsmAI 119 GAGAC GTCTC(1/5)
BsmFI 430 GTCCC GGGAC(10/14)
Bsp1286I 233 GAGCAC G[AGT]GC[ACT]^C
BspEI 477 TCCGGA T^CCGGA
BspHI 53 TCATGA T^CATGA
BsrI 219 CCAGT ACTGG(1/-1)
Bsu36I 706 CCTAAGG CC^T[ACGT]AGG
DraI 610 TTTAAA TTT^AAA
Eco81I 706 CCTAAGG CC^T[ACGT]AGG
EcoNI 692 CCTTTAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 717 GATATC GAT^ATC
EcoT14I 66 CCATGG C^C[AT][AT]GG
Fnu4HI 303 GCAGC GC^[ACGT]GC
HapII 478 CCGG C^CGG
HgaI 180 GCGTC GACGC(5/10)
HpaII 478 CCGG C^CGG
ItaI 303 GCAGC GC^[ACGT]GC
MaeI 230 CTAG C^TAG
MamI 713 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 477 TCCGGA T^CCGGA
MslI 67 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 425 CAGCTG C[AC]G^C[GT]G
MspI 478 CCGG C^CGG
MwoI 630 GCTACTATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 66 CCATGG C^CATGG
NlaIV 160 GGTGCC GG[ACGT]^[ACGT]CC
PvuII 425 CAGCTG CAG^CTG
RcaI 53 TCATGA T^CATGA
ScaI 437 AGTACT AGT^ACT
SspI 727 AATATT AAT^ATT
StyI 66 CCATGG C^C[AT][AT]GG
TfiI 778 GAATC G^A[AT]TC
TseI 303 GCAGC G^C[AT]GC
XbaI 229 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 9 enzymes) #
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AfaI 438 GTAC GT^AC
772 GTAC
BsrDI 366 CATTGC GCAATG(2/0)
470 GCAATG
Cac8I 39 GCAAGC GC[ACGT]^[ACGT]GC
306 GCAAGC
DsaI 66 CCATGG C^C[GA][CT]GG
156 CCGTGG
MaeIII 152 GTAAC ^GT[ACGT]AC
284 GTAAC
PleI 318 GACTC GAGTC(4/5)
702 GACTC
RsaI 438 GTAC GT^AC
772 GTAC
SfcI 734 CTATAG C^T[GA][CT]AG
755 CTACAG
TspRI 389 CACTG CA[GC]TG[ACGT][ACGT]^
768 CACTG
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BsaJI BsiYI BslI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EcoRII FokI HinfI MboI MboII
MflI MnlI MseI MvaI NdeII
NlaIII Sau3AI Sau96I ScrFI SfaNI
Tru9I Tsp509I XhoII