Restriction map for
pFN21AE0896
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstYI BstZ17I Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FseI HaeII
HapII HgaI Hin1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeII MfeI MflI MluI MroI
MspA1I MspI MunI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI Sau96I SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 544 CGCG CG^CG
Alw44I 473 GTGCAC G^TGCAC
AlwI 589 GATCC GGATC(4/5)
ApaLI 473 GTGCAC G^TGCAC
Asp700I 756 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 17 TGCGCA TGC^GCA
BbsI 694 GTCTTC GAAGAC(2/6)
BclI 644 TGATCA T^GATCA
BpmI 743 CTGGAG CTGGAG(16/14)
BpuAI 694 GTCTTC GAAGAC(2/6)
BsaBI 642 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 809 GGTCTC GGTCTC(1/5)
BseRI 115 GAGGAG GAGGAG(10/8)
BsmFI 461 GTCCC GGGAC(10/14)
BspMI 176 ACCTGC ACCTGC(4/8)
BstEII 159 GGTCACC G^GT[ACGT]ACC
BstPI 159 GGTCACC G^GT[ACGT]ACC
BstUI 544 CGCG CG^CG
BstXI 287 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 18 GCGC GCG^C
EcoO65I 159 GGTCACC G^GT[ACGT]ACC
FauI 542 CCCGC CCCGC(4/6)
FbaI 644 TGATCA T^GATCA
FspI 17 TGCGCA TGC^GCA
HhaI 18 GCGC GCG^C
HinP1I 18 GCGC G^CGC
HindIII 607 AAGCTT A^AGCTT
MaeIII 160 GTCAC ^GT[ACGT]AC
MamI 642 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 253 TTAA T^TAA
MvnI 544 CGCG CG^CG
NdeI 407 CATATG CA^TATG
NlaIV 173 GGAACC GG[ACGT]^[ACGT]CC
NspI 149 GCATGT [GA]CATG^[CT]
SapI 798 GCTCTTC GCTCTTC(1/4)
SexAI 155 ACCTGGT A^CC[AT]GGT
SfcI 851 CTACAG C^T[GA][CT]AG
SmlI 924 CTTGAG C^T[CT][GA]AG
StuI 758 AGGCCT AGG^CCT
Tru9I 253 TTAA T^TAA
Tsp45I 160 GTCAC ^GT[GC]AC
XmnI 756 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 153 GTAC GT^AC
228 GTAC
AspHI 473 GTGCAC G[AT]GC[AT]^C
596 GTGCTC
BbvI 120 GCAGC GCAGC(8/12)
871 GCTGC
BfaI 94 CTAG C^TAG
593 CTAG
BsiHKAI 473 GTGCAC G[AT]GC[AT]^C
596 GTGCTC
BsiYI 164 CCATGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
414 CCGCTATGTGG
BslI 164 CCATGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
414 CCGCTATGTGG
BsmAI 935 GAGAC GTCTC(1/5)
810 GTCTC
DdeI 104 CTGAG C^T[ACGT]AG
618 CTCAG
DsaI 164 CCATGG C^C[GA][CT]GG
653 CCATGG
EarI 125 CTCTTC CTCTTC(1/4)
799 CTCTTC
Fnu4HI 120 GCAGC GC^[ACGT]GC
871 GCTGC
ItaI 120 GCAGC GC^[ACGT]GC
871 GCTGC
Ksp632I 125 CTCTTC CTCTTC(1/4)
799 CTCTTC
MaeI 94 CTAG C^TAG
593 CTAG
NcoI 164 CCATGG C^CATGG
653 CCATGG
RsaI 153 GTAC GT^AC
228 GTAC
TseI 120 GCAGC G^C[AT]GC
871 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BalI BmyI BsaJI
Bsp1286I BssKI BstF5I BstNI Cac8I
DpnII EaeI EcoRII EcoT14I FokI
HaeIII HinfI HphI MboI MboII
MluNI MnlI MscI MslI MvaI
MwoI NdeII NlaIII PleI Sau3AI
ScrFI SfaNI StyI TspRI