(Circular) (Six-base) MAPSORT of: pFN21AE0896.seq Check: 7410 from: 1 to: 5695 pFN21AE0896 5695 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3038 1937 Size: 1101 4594 Acc65I G'GTAC_C Cuts at: 3016 3016 Size: 5695 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2630 3758 5142 5410 1632 Size: 998 1128 1384 268 1917 Fragments arranged by size: 1917 1384 1128 998 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 4594 5571 1203 Size: 3391 977 1327 Fragments arranged by size: 3391 1327 977 AgeI A'CCGG_T Cuts at: 4526 4526 Size: 5695 AhdI GACnn_n'nnGTC Cuts at: 4424 4424 Size: 5695 AleI CACnn'nnGTG Cuts at: 1166 2171 1166 Size: 1005 4690 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5695 AlwNI CAG_nnn'CTG Cuts at: 3438 5010 3438 Size: 1572 4123 ApaLI G'TGCA_C Cuts at: 2478 3752 4908 2478 Size: 1274 1156 3265 Fragments arranged by size: 3265 1274 1156 ApoI r'AATT_y Cuts at: 1493 3004 3230 3382 5491 1493 Size: 1511 226 152 2109 1697 Fragments arranged by size: 2109 1697 1511 226 152 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 3020 1870 Size: 73 1077 4545 Fragments arranged by size: 4545 1077 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2619 2652 2619 Size: 33 5662 BamHI G'GATC_C Cuts at: 3025 3392 3025 Size: 367 5328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3016 4471 618 Size: 325 217 627 1229 1455 1842 Fragments arranged by size: 1842 1455 1229 627 325 217 BanII G_rGCy'C Cuts at: 1391 3014 1391 Size: 1623 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2692 928 Size: 971 793 3931 Fragments arranged by size: 3931 971 793 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3886 3920 1903 Size: 34 1949 34 3678 Fragments arranged by size: 3678 1949 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3587 4797 3587 Size: 1210 4485 BclI T'GATC_A Cuts at: 1548 2649 1548 Size: 1101 4594 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4306 136 Size: 107 122 71 631 605 2634 1525 Fragments arranged by size: 2634 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5690 5690 Size: 5695 BlpI GC'TnA_GC Cuts at: 3112 3112 Size: 5695 Bme1580I G_kGCm'C Cuts at: 1790 2482 2566 3756 4912 1790 Size: 692 84 1190 1156 2573 Fragments arranged by size: 2573 1190 1156 692 84 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5695 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3426 4384 442 Size: 416 53 83 399 24 2009 958 1753 Fragments arranged by size: 2009 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2769 4355 1239 Size: 1530 1586 2579 Fragments arranged by size: 2579 1586 1530 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2950 3820 4685 4983 2950 Size: 870 865 298 3662 Fragments arranged by size: 3662 870 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2820 4358 882 Size: 617 1321 1538 2219 Fragments arranged by size: 2219 1538 1321 617 BsaAI yAC'GTr Cuts at: 493 1204 3486 493 Size: 711 2282 2702 Fragments arranged by size: 2702 2282 711 BsaBI GATnn'nnATC Cuts at: 2651 5689 2651 Size: 3038 2657 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3502 3884 4519 275 Size: 53 83 186 936 1969 382 635 1451 Fragments arranged by size: 1969 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4128 4526 4800 4947 5358 4128 Size: 398 274 147 411 4465 Fragments arranged by size: 4465 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5470 Fragments arranged by size: 5470 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 1186 Size: 949 4746 BseYI C'CCAG_C Cuts at: 2469 2488 2499 4898 5394 2469 Size: 19 11 2399 496 2770 Fragments arranged by size: 2770 2399 496 19 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5122 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3145 3906 4934 1540 Size: 275 186 1144 761 1028 2301 Fragments arranged by size: 2301 1144 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2482 2605 3756 3841 4912 2482 Size: 123 1151 85 1071 3265 Fragments arranged by size: 3265 1151 1071 123 85 BsmI GAATG_Cn' Cuts at: 3206 3299 3206 Size: 93 5602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2482 2566 2605 3014 3756 3841 4912 Size: 399 692 84 39 409 742 85 1071 Cuts at: 4912 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 692 409 399 85 84 39 BspEI T'CCGG_A Cuts at: 5358 5358 Size: 5695 BspHI T'CATG_A Cuts at: 3584 5314 3584 Size: 1730 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2190 3050 844 Size: 1346 860 3489 Fragments arranged by size: 3489 1346 860 BsrBI CCG'CTC Cuts at: 1394 1823 3582 1394 Size: 429 1759 3507 Fragments arranged by size: 3507 1759 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4190 4364 65 Size: 1183 2942 174 1396 Fragments arranged by size: 2942 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4339 4526 1700 Size: 110 2529 187 2869 Fragments arranged by size: 2869 2529 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5695 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5695 BssSI C'ACGA_G Cuts at: 3755 4767 3755 Size: 1012 4683 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5695 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 2164 1184 Size: 980 4715 BstXI CCAn_nnnn'nTGG Cuts at: 2299 2299 Size: 5695 BstYI r'GATC_y Cuts at: 1972 3025 3392 3777 3794 5246 5332 5344 5690 Size: 1053 367 385 17 1452 86 12 346 Cuts at: 5690 1972 Size: 1977 Fragments arranged by size: 1977 1452 1053 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2169 2658 513 Size: 552 939 165 489 3550 Fragments arranged by size: 3550 939 552 489 165 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4956 Fragments arranged by size: 4956 683 56 BtsI GCAGTG_nn' Cuts at: 3211 4005 4025 3211 Size: 794 20 4881 Fragments arranged by size: 4881 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5660 DraI TTT'AAA Cuts at: 2999 2999 Size: 5695 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4702 809 Size: 507 3386 1802 Fragments arranged by size: 3386 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2114 2196 2244 Size: 54 1425 50 275 18 284 82 48 Cuts at: 2244 2301 2427 3142 4031 5660 8 Size: 57 126 715 889 1629 43 Fragments arranged by size: 1629 1425 889 715 284 275 126 82 57 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3142 1537 Size: 275 1330 4090 Fragments arranged by size: 4090 1330 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2136 2810 3625 1353 Size: 783 674 815 3423 Fragments arranged by size: 3423 815 783 674 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4282 4656 4802 387 Size: 1064 138 2693 374 146 1280 Fragments arranged by size: 2693 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2630 2769 3758 4355 5142 5410 1239 Size: 393 998 139 989 597 787 268 1524 Fragments arranged by size: 1524 998 989 787 597 393 268 139 EcoRI G'AATT_C Cuts at: 3004 3382 3004 Size: 378 5317 FspI TGC'GCA Cuts at: 2024 4201 2024 Size: 2177 3518 HaeII r_GCGC'y Cuts at: 1678 4842 1678 Size: 3164 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4336 4368 4410 4442 1373 Size: 32 135 32 2764 32 42 32 2626 Fragments arranged by size: 2764 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3039 3285 669 Size: 988 281 1101 246 3079 Fragments arranged by size: 3079 1101 988 281 246 HindIII A'AGCT_T Cuts at: 748 2612 748 Size: 1864 3831 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KpnI G_GTAC'C Cuts at: 3020 3020 Size: 5695 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 5571 5571 Size: 5695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4809 4993 1305 Size: 3504 184 2007 Fragments arranged by size: 3504 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2116 2198 2246 2303 2429 Size: 54 1425 343 284 82 48 57 126 Cuts at: 2429 10 Size: 3276 Fragments arranged by size: 3276 1425 343 284 126 82 57 54 48 MslI CAynn'nnrTG Cuts at: 518 1166 2171 2459 2657 3653 4012 4171 518 Size: 648 1005 288 198 996 359 159 2042 Fragments arranged by size: 2042 1005 996 648 359 288 198 159 MspA1I CmG'CkG Cuts at: 1606 3111 3788 4936 5181 1606 Size: 1505 677 1148 245 2120 Fragments arranged by size: 2120 1505 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2169 2658 513 Size: 552 939 165 489 3550 Fragments arranged by size: 3550 939 552 489 165 NdeI CA'TA_TG Cuts at: 387 2413 387 Size: 2026 3669 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5695 NotI GC'GGCC_GC Cuts at: 3142 3142 Size: 5695 NspI r_CATG'y Cuts at: 1132 1229 2158 3053 4598 1132 Size: 97 929 895 1545 2229 Fragments arranged by size: 2229 1545 929 895 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5695 PciI A'CATG_T Cuts at: 4594 4594 Size: 5695 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5695 PfoI T'CCnGG_A Cuts at: 2746 2746 Size: 5695 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5277 5309 3764 Size: 32 1481 32 4150 Fragments arranged by size: 4150 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5695 PsiI TTA'TAA Cuts at: 3265 3265 Size: 5695 PstI C_TGCA'G Cuts at: 830 3047 830 Size: 2217 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 SalI G'TCGA_C Cuts at: 1936 3037 1936 Size: 1101 4594 SapI GCTCTTCn'nnn_ Cuts at: 2810 2810 Size: 5695 SbfI CC_TGCA'GG Cuts at: 3047 3047 Size: 5695 ScaI AGT'ACT Cuts at: 3943 5655 3943 Size: 1712 3983 SexAI A'CCwGG_T Cuts at: 2160 2160 Size: 5695 SfcI C'TryA_G Cuts at: 826 1045 2856 3043 4178 4859 5050 826 Size: 219 1811 187 1135 681 191 1471 Fragments arranged by size: 1811 1471 1135 681 219 191 187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5695 SmaI CCC'GGG Cuts at: 1872 3022 1872 Size: 1150 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2929 3799 4700 4962 820 Size: 197 926 986 870 901 262 1553 Fragments arranged by size: 1553 986 926 901 870 262 197 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 3053 1132 Size: 1921 3774 SspI AAT'ATT Cuts at: 5 52 3619 5 Size: 47 3567 2081 Fragments arranged by size: 3567 2081 47 StuI AGG'CCT Cuts at: 1517 2765 1517 Size: 1248 4447 StyI C'CwwG_G Cuts at: 513 1065 2004 2149 2169 2658 3134 513 Size: 552 939 145 20 489 476 3074 Fragments arranged by size: 3074 939 552 489 476 145 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2331 2480 2491 2813 3722 3739 3892 Size: 1255 751 149 11 322 909 17 153 Cuts at: 3892 4077 4522 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 909 751 445 322 185 153 149 17 11 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2232 3941 5653 Size: 274 80 33 51 1443 255 1709 1712 Cuts at: 5653 96 Size: 138 Fragments arranged by size: 1712 1709 1443 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5655 5687 5655 Size: 32 5663 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5695 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5695 XmaI C'CCGG_G Cuts at: 1870 3020 1870 Size: 1150 4545 XmnI GAAnn'nnTTC Cuts at: 2765 3824 2765 Size: 1059 4636 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI