(Circular) (Six-base) MAPSORT of: pFN21AE0895.seq Check: 2421 from: 1 to: 5644 pFN21AE0895 5644 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2732 2732 Size: 5644 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2987 1937 Size: 1050 4594 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3707 5091 5359 1632 Size: 2075 1384 268 1917 Fragments arranged by size: 2075 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 4543 5520 1203 Size: 3340 977 1327 Fragments arranged by size: 3340 1327 977 AgeI A'CCGG_T Cuts at: 2367 4475 2367 Size: 2108 3536 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 2582 2807 1166 Size: 1416 225 4003 Fragments arranged by size: 4003 1416 225 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5644 AlwNI CAG_nnn'CTG Cuts at: 3387 4959 3387 Size: 1572 4072 ApaLI G'TGCA_C Cuts at: 2569 3701 4857 2569 Size: 1132 1156 3356 Fragments arranged by size: 3356 1156 1132 ApoI r'AATT_y Cuts at: 1493 2953 3179 3331 5440 1493 Size: 1460 226 152 2109 1697 Fragments arranged by size: 2109 1697 1460 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2969 1870 Size: 73 1026 4545 Fragments arranged by size: 4545 1026 73 BamHI G'GATC_C Cuts at: 2974 3341 2974 Size: 367 5277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2441 2524 2965 4420 618 Size: 325 217 627 654 83 441 1455 1842 Fragments arranged by size: 1842 1455 654 627 441 325 217 83 BanII G_rGCy'C Cuts at: 1391 2905 2963 1391 Size: 1514 58 4072 Fragments arranged by size: 4072 1514 58 BbeI G_GCGC'C Cuts at: 2445 2445 Size: 5644 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4673 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2550 2584 3835 3869 1903 Size: 34 613 34 1251 34 3678 Fragments arranged by size: 3678 1251 613 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2357 2450 4255 Size: 107 122 71 631 605 685 93 1805 Cuts at: 4255 136 Size: 1525 Fragments arranged by size: 1805 1525 685 631 605 122 107 93 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 2573 3705 4861 1790 Size: 783 1132 1156 2573 Fragments arranged by size: 2573 1156 1132 783 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4304 1239 Size: 3065 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2899 3769 4634 4932 2899 Size: 870 865 298 3611 Fragments arranged by size: 3611 870 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2883 4307 882 Size: 617 1384 1424 2219 Fragments arranged by size: 2219 1424 1384 617 BsaAI yAC'GTr Cuts at: 493 1204 3435 493 Size: 711 2231 2702 Fragments arranged by size: 2702 2231 711 BsaBI GATnn'nnATC Cuts at: 2597 5638 2597 Size: 3041 2603 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2442 2775 3451 3833 Size: 53 83 186 936 909 333 676 382 Cuts at: 3833 4468 275 Size: 635 1451 Fragments arranged by size: 1451 936 909 676 635 382 333 186 83 53 BsaWI w'CCGG_w Cuts at: 2367 4077 4475 4749 4896 5307 2367 Size: 1710 398 274 147 411 2704 Fragments arranged by size: 2704 1710 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5419 Fragments arranged by size: 5419 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2782 1186 Size: 1596 4048 BseYI C'CCAG_C Cuts at: 4847 5343 4847 Size: 496 5148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5071 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2573 3705 3790 4861 2573 Size: 1132 85 1071 3356 Fragments arranged by size: 3356 1132 1071 85 BsmI GAATG_Cn' Cuts at: 3155 3248 3155 Size: 93 5551 BsmBI CGTCTCn'nnnn_ Cuts at: 2069 2504 2069 Size: 435 5209 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2573 2905 2963 3705 3790 4861 1391 Size: 399 783 332 58 742 85 1071 2174 Fragments arranged by size: 2174 1071 783 742 399 332 85 58 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 2409 2679 3533 5263 2409 Size: 270 854 1730 2790 Fragments arranged by size: 2790 1730 854 270 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 2732 2999 844 Size: 1295 593 267 3489 Fragments arranged by size: 3489 1295 593 267 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2367 4288 4475 1700 Size: 110 557 1921 187 2869 Fragments arranged by size: 2869 1921 557 187 110 BsrGI T'GTAC_A Cuts at: 96 2835 96 Size: 2739 2905 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstXI CCAn_nnnn'nTGG Cuts at: 2324 2324 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2592 2974 3341 3726 3743 5195 5281 5293 Size: 620 382 367 385 17 1452 86 12 Cuts at: 5293 5639 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 620 385 382 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4153 Fragments arranged by size: 4153 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 3160 3954 3974 3160 Size: 794 20 4830 Fragments arranged by size: 4830 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2617 2652 529 Size: 35 2053 35 3521 Fragments arranged by size: 3521 2053 35 35 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2193 2223 Size: 54 1425 50 275 18 315 48 30 Cuts at: 2223 2376 2673 3091 3980 5609 8 Size: 153 297 418 889 1629 43 Fragments arranged by size: 1629 1425 889 418 315 297 275 153 54 50 48 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2759 3574 1353 Size: 1406 815 3423 Fragments arranged by size: 3423 1406 815 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3707 4304 5091 5359 1239 Size: 393 2075 597 787 268 1524 Fragments arranged by size: 2075 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2686 2686 Size: 5644 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2731 2731 Size: 5644 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 2445 4791 1678 Size: 767 2346 2531 Fragments arranged by size: 2531 2346 767 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2482 2514 4285 4317 4359 Size: 32 135 32 910 32 1771 32 42 Cuts at: 4359 4391 1373 Size: 32 2626 Fragments arranged by size: 2626 1771 910 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2920 2988 3234 669 Size: 988 281 982 68 246 3079 Fragments arranged by size: 3079 988 982 281 246 68 HindIII A'AGCT_T Cuts at: 748 2820 748 Size: 2072 3572 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KasI G'GCGC_C Cuts at: 2441 2441 Size: 5644 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2584 4758 4942 1305 Size: 1279 2174 184 2007 Fragments arranged by size: 2174 2007 1279 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2147 2195 2225 2378 2675 Size: 54 1425 343 315 48 30 153 297 Cuts at: 2675 10 Size: 2979 Fragments arranged by size: 2979 1425 343 315 297 153 54 48 30 MslI CAynn'nnrTG Cuts at: 518 1166 2120 2177 2582 2669 2807 2852 3602 Size: 648 954 57 405 87 138 45 750 Cuts at: 3602 3961 4120 518 Size: 359 159 2042 Fragments arranged by size: 2042 954 750 648 405 359 159 138 87 57 45 MspA1I CmG'CkG Cuts at: 1606 3060 3737 4885 5130 1606 Size: 1454 677 1148 245 2120 Fragments arranged by size: 2120 1454 1148 677 245 NarI GG'CG_CC Cuts at: 2442 2442 Size: 5644 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4153 Fragments arranged by size: 4153 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 2107 3002 4547 1132 Size: 97 878 895 1545 2229 Fragments arranged by size: 2229 1545 895 878 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 4543 4543 Size: 5644 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2686 2686 Size: 5644 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PstI C_TGCA'G Cuts at: 830 2698 2729 2996 830 Size: 1868 31 267 3478 Fragments arranged by size: 3478 1868 267 31 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SexAI A'CCwGG_T Cuts at: 2109 2109 Size: 5644 SfcI C'TryA_G Cuts at: 826 1045 2694 2725 2872 2992 4127 4808 4999 Size: 219 1649 31 147 120 1135 681 191 Cuts at: 4999 826 Size: 1471 Fragments arranged by size: 1649 1471 1135 681 219 191 147 120 31 SfoI GGC'GCC Cuts at: 2443 2443 Size: 5644 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2878 3748 4649 4911 820 Size: 197 926 935 870 901 262 1553 Fragments arranged by size: 1553 935 926 901 870 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2004 3083 513 Size: 552 939 1079 3074 Fragments arranged by size: 3074 1079 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2785 3671 3688 3841 4026 4471 325 Size: 1255 1205 886 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1205 886 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2307 2835 Size: 274 80 33 51 1443 204 126 528 Cuts at: 2835 3890 5602 96 Size: 1055 1712 138 Fragments arranged by size: 1712 1443 1055 528 274 204 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 2339 1316 Size: 1023 4621 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2203 2203 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 2714 2864 3773 2714 Size: 150 909 4585 Fragments arranged by size: 4585 909 150 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SrfI SwaI