Restriction map for
pFN21AE0895
(Linear) (Six-base) MAPSORT of: KIEE0895.seq Check: 636 from: 1 to: 939
KIEE0895.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 939
Size: 727 212
AgeI A'CCGG_T
Cuts at: 0 362 939
Size: 362 577
AleI CACnn'nnGTG
Cuts at: 0 577 802 939
Size: 577 225 137
Fragments arranged by size:
577 225 137
ApaLI G'TGCA_C
Cuts at: 0 564 939
Size: 564 375
BanI G'GyrC_C
Cuts at: 0 436 519 939
Size: 436 83 420
Fragments arranged by size:
436 420 83
BanII G_rGCy'C
Cuts at: 0 900 939
Size: 900 39
BbeI G_GCGC'C
Cuts at: 0 440 939
Size: 440 499
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 545 579 939
Size: 545 34 360
Fragments arranged by size:
545 360 34
BglI GCCn_nnn'nGGC
Cuts at: 0 352 445 939
Size: 352 93 494
Fragments arranged by size:
494 352 93
Bme1580I G_kGCm'C
Cuts at: 0 568 939
Size: 568 371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 894 939
Size: 894 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 878 939
Size: 878 61
BsaBI GATnn'nnATC
Cuts at: 0 592 939
Size: 592 347
BsaHI Gr'CG_yC
Cuts at: 0 437 770 939
Size: 437 333 169
Fragments arranged by size:
437 333 169
BsaWI w'CCGG_w
Cuts at: 0 362 939
Size: 362 577
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 777 939
Size: 777 162
BsiHKAI G_wGCw'C
Cuts at: 0 568 939
Size: 568 371
BsmBI CGTCTCn'nnnn_
Cuts at: 0 64 499 939
Size: 64 435 440
Fragments arranged by size:
440 435 64
Bsp1286I G_dGCh'C
Cuts at: 0 568 900 939
Size: 568 332 39
Fragments arranged by size:
568 332 39
BspHI T'CATG_A
Cuts at: 0 404 674 939
Size: 404 270 265
Fragments arranged by size:
404 270 265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 727 939
Size: 134 593 212
Fragments arranged by size:
593 212 134
BsrFI r'CCGG_y
Cuts at: 0 362 939
Size: 362 577
BsrGI T'GTAC_A
Cuts at: 0 830 939
Size: 830 109
BstXI CCAn_nnnn'nTGG
Cuts at: 0 319 939
Size: 319 620
BstYI r'GATC_y
Cuts at: 0 587 939
Size: 587 352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 612 647 939
Size: 612 35 292
Fragments arranged by size:
612 292 35
EaeI y'GGCC_r
Cuts at: 0 140 188 218 371 668 939
Size: 140 48 30 153 297 271
Fragments arranged by size:
297 271 153 140 48 30
EarI CTCTTCn'nnn_
Cuts at: 0 754 939
Size: 754 185
EcoO109I rG'GnC_Cy
Cuts at: 0 681 939
Size: 681 258
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 726 939
Size: 726 213
HaeII r_GCGC'y
Cuts at: 0 440 939
Size: 440 499
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 477 509 939
Size: 477 32 430
Fragments arranged by size:
477 430 32
HincII GTy'rAC
Cuts at: 0 915 939
Size: 915 24
HindIII A'AGCT_T
Cuts at: 0 815 939
Size: 815 124
KasI G'GCGC_C
Cuts at: 0 436 939
Size: 436 503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 939
Size: 579 360
MscI TGG'CCA
Cuts at: 0 142 190 220 373 670 939
Size: 142 48 30 153 297 269
Fragments arranged by size:
297 269 153 142 48 30
MslI CAynn'nnrTG
Cuts at: 0 115 172 577 664 802 847 939
Size: 115 57 405 87 138 45 92
Fragments arranged by size:
405 138 115 92 87 57 45
NarI GG'CG_CC
Cuts at: 0 437 939
Size: 437 502
NspI r_CATG'y
Cuts at: 0 102 939
Size: 102 837
PpuMI rG'GwC_Cy
Cuts at: 0 681 939
Size: 681 258
PstI C_TGCA'G
Cuts at: 0 693 724 939
Size: 693 31 215
Fragments arranged by size:
693 215 31
SexAI A'CCwGG_T
Cuts at: 0 104 939
Size: 104 835
SfcI C'TryA_G
Cuts at: 0 689 720 867 939
Size: 689 31 147 72
Fragments arranged by size:
689 147 72 31
SfoI GGC'GCC
Cuts at: 0 438 939
Size: 438 501
SmlI C'TyrA_G
Cuts at: 0 873 939
Size: 873 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 780 939
Size: 780 159
TatI w'GTAC_w
Cuts at: 0 176 302 830 939
Size: 176 126 528 109
Fragments arranged by size:
528 176 126 109
Tth111I GACn'n_nGTC
Cuts at: 0 334 939
Size: 334 605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 939
Size: 198 741
XmnI GAAnn'nnTTC
Cuts at: 0 709 859 939
Size: 709 150 80
Fragments arranged by size:
709 150 80
Enzymes that do cut:
AarI AgeI AleI ApaLI BanI BanII BbeI BcgI
BglI Bme1580I BpuEI BsaI BsaBI BsaHI BsaWI BseRI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI BstXI
BstYI CspCI EaeI EarI EcoO109I FalI HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI NarI NspI
PpuMI PstI SexAI SfcI SfoI SmlI TaqII TatI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
BpmI Bpu10I BsaAI BsaXI BseYI BsgI BsiEI BsiWI
BsmI BspEI BsrBI BsrDI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EagI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FseI FspI FspAI HpaI KpnI MfeI MluI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TstI XbaI XhoI XmaI
ZraI