Restriction map for
pFN21AE0890
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspHI AspI AvaI AviII
AvrII BanI BbeI BbrPI BbsI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRI EcoRV EcoT14I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspI StuI
StyI SwaI TaqI Tth111I TthHB8I
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AciI 390 CCGC CCGC(-3/-1)
AhdI 608 GACAGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 873 CCTAAGG CC^T[ACGT]AGG
Asp700I 855 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 608 GACAGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 45 GGACC G^G[AT]CC
BamHI 446 GGATCC G^GATCC
BanII 896 GAGCCC G[GA]GC[CT]^C
BbvCI 186 CCTCAGC CCTCAGC(-5/-2)
BbvI 66 GCAGC GCAGC(8/12)
BmyI 896 GAGCCC G[AGT]GC[ACT]^C
BsaJI 899 CCCTGG C^C[ACGT][ACGT]GG
BsiEI 360 CGATCG CG[GA][CT]^CG
Bsp1286I 896 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 830 TGTACA T^GTACA
BspHI 404 TCATGA T^CATGA
BspMI 125 ACCTGC ACCTGC(4/8)
BsrDI 848 CATTGC GCAATG(2/0)
BsrGI 830 TGTACA T^GTACA
BstEII 666 GGTCACC G^GT[ACGT]ACC
BstPI 666 GGTCACC G^GT[ACGT]ACC
BstYI 446 GGATCC [GA]^GATC[CT]
Bsu36I 873 CCTAAGG CC^T[ACGT]AGG
Eam1105I 608 GACAGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 873 CCTAAGG CC^T[ACGT]AGG
EcoO65I 666 GGTCACC G^GT[ACGT]ACC
EcoT22I 636 ATGCAT ATGCA^T
Fnu4HI 66 GCAGC GC^[ACGT]GC
HapII 147 CCGG C^CGG
HindIII 707 AAGCTT A^AGCTT
HpaII 147 CCGG C^CGG
ItaI 66 GCAGC GC^[ACGT]GC
MaeII 812 ACGT A^CGT
MflI 446 GGATCC [GA]^GATC[CT]
MseI 247 TTAA T^TAA
MspA1I 493 CAGCTG C[AC]G^C[GT]G
MspI 147 CCGG C^CGG
NsiI 636 ATGCAT ATGCA^T
PvuI 360 CGATCG CGAT^CG
PvuII 493 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
SpeI 429 ACTAGT A^CTAGT
SphI 251 GCATGC GCATG^C
SspBI 830 TGTACA T^GTACA
Tru9I 247 TTAA T^TAA
TseI 66 GCAGC G^C[AT]GC
XbaI 327 TCTAGA T^CTAGA
XhoII 446 GGATCC [GA]^GATC[CT]
XmnI 855 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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BclI 107 TGATCA T^GATCA
839 TGATCA
BfaI 328 CTAG C^TAG
430 CTAG
BpmI 798 CTCCAG CTGGAG(16/14)
901 CTGGAG
BseRI 37 CTCCTC GAGGAG(10/8)
481 CTCCTC
BsrI 791 CCAGT ACTGG(1/-1)
800 CCAGT
Cac8I 69 GCAGGC GC[ACGT]^[ACGT]GC
251 GCATGC
Cfr13I 45 GGACC G^G[ACGT]CC
489 GGCCC
EarI 910 CTCTTC CTCTTC(1/4)
3 GAAGAG
FbaI 107 TGATCA T^GATCA
839 TGATCA
Ksp632I 910 CTCTTC CTCTTC(1/4)
3 GAAGAG
MaeI 328 CTAG C^TAG
430 CTAG
MwoI 60 GCCAGAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
141 GCTCATCCGGC
NspI 98 GCATGT [GA]CATG^[CT]
251 GCATGC
PleI 154 GACTC GAGTC(4/5)
908 GACTC
Sau96I 45 GGACC G^G[ACGT]CC
489 GGCCC
SfaNI 638 GCATC GCATC(5/9)
698 GCATC
SmlI 553 CTCAAG C^T[CT][GA]AG
676 CTCAAG
TfiI 12 GAATC G^A[AT]TC
855 GAATC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI AlwI BalI BsiYI
BslI BsmAI BsmFI BssKI BstF5I
BstNI DdeI DpnII EaeI EcoRII
FokI HaeIII HinfI HphI MaeIII
MboI MboII MluNI MnlI MscI
MslI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI Tsp45I Tsp509I
TspRI