(Circular) (Six-base) MAPSORT of: pFN21AE0888.seq Check: 7205 from: 1 to: 5770 pFN21AE0888 5770 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2492 2492 Size: 5770 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3580 278 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3113 1937 Size: 1176 4594 Acc65I G'GTAC_C Cuts at: 3091 3091 Size: 5770 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2579 3833 5217 5485 1632 Size: 947 1254 1384 268 1917 Fragments arranged by size: 1917 1384 1254 947 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5573 AflIII A'CryG_T Cuts at: 1203 4669 5646 1203 Size: 3466 977 1327 Fragments arranged by size: 3466 1327 977 AgeI A'CCGG_T Cuts at: 4601 4601 Size: 5770 AhdI GACnn_n'nnGTC Cuts at: 4499 4499 Size: 5770 AleI CACnn'nnGTG Cuts at: 1166 2744 1166 Size: 1578 4192 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5770 AlwNI CAG_nnn'CTG Cuts at: 3513 5085 3513 Size: 1572 4198 ApaLI G'TGCA_C Cuts at: 3827 4983 3827 Size: 1156 4614 ApoI r'AATT_y Cuts at: 1493 2036 3079 3305 3457 5566 1493 Size: 543 1043 226 152 2109 1697 Fragments arranged by size: 2109 1697 1043 543 226 152 AseI AT'TA_AT Cuts at: 160 1032 4324 160 Size: 872 3292 1606 Fragments arranged by size: 3292 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 2697 3095 1870 Size: 73 754 398 4545 Fragments arranged by size: 4545 754 398 73 AvrII C'CTAG_G Cuts at: 2942 2942 Size: 5770 BamHI G'GATC_C Cuts at: 3100 3467 3100 Size: 367 5403 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2624 3091 4546 618 Size: 325 217 627 837 467 1455 1842 Fragments arranged by size: 1842 1455 837 627 467 325 217 BanII G_rGCy'C Cuts at: 1391 3089 1391 Size: 1698 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4799 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3961 3995 1903 Size: 34 2024 34 3678 Fragments arranged by size: 3678 2024 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3662 4872 3662 Size: 1210 4560 BclI T'GATC_A Cuts at: 1548 2454 1548 Size: 906 4864 BfrBI ATG'CAT Cuts at: 3013 3013 Size: 5770 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4381 136 Size: 107 122 71 631 605 2709 1525 Fragments arranged by size: 2709 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5765 5765 Size: 5770 BlpI GC'TnA_GC Cuts at: 3187 3187 Size: 5770 Bme1580I G_kGCm'C Cuts at: 1790 2627 3831 4987 1790 Size: 837 1204 1156 2573 Fragments arranged by size: 2573 1204 1156 837 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3501 4459 442 Size: 416 53 83 399 24 2084 958 1753 Fragments arranged by size: 2084 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2252 1056 Size: 1196 4574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2030 2450 3051 4430 1239 Size: 791 420 601 1379 2579 Fragments arranged by size: 2579 1379 791 601 420 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3895 4760 5058 3895 Size: 865 298 4607 Fragments arranged by size: 4607 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4433 882 Size: 617 2934 2219 Fragments arranged by size: 2934 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3561 493 Size: 711 2357 2702 Fragments arranged by size: 2702 2357 711 BsaBI GATnn'nnATC Cuts at: 2967 5764 2967 Size: 2797 2973 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3577 3959 4594 275 Size: 53 83 186 936 2044 382 635 1451 Fragments arranged by size: 2044 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4203 4601 4875 5022 5433 4203 Size: 398 274 147 411 4540 Fragments arranged by size: 4540 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5545 Fragments arranged by size: 5545 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2794 3048 1186 Size: 886 722 254 3908 Fragments arranged by size: 3908 886 722 254 BseYI C'CCAG_C Cuts at: 2437 2798 3050 4973 5469 2437 Size: 361 252 1923 496 2738 Fragments arranged by size: 2738 1923 496 361 252 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5197 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2368 3220 3981 5009 1540 Size: 275 186 367 852 761 1028 2301 Fragments arranged by size: 2301 1028 852 761 367 275 186 BsiHKAI G_wGCw'C Cuts at: 2417 3831 3916 4987 2417 Size: 1414 85 1071 3200 Fragments arranged by size: 3200 1414 1071 85 BsmI GAATG_Cn' Cuts at: 3281 3374 3281 Size: 93 5677 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2417 2627 3089 3831 3916 4987 1391 Size: 399 627 210 462 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 627 462 399 210 85 BspEI T'CCGG_A Cuts at: 5433 5433 Size: 5770 BspHI T'CATG_A Cuts at: 3659 5389 3659 Size: 1730 4040 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2492 3125 844 Size: 1648 633 3489 Fragments arranged by size: 3489 1648 633 BsrBI CCG'CTC Cuts at: 1394 1823 3657 1394 Size: 429 1834 3507 Fragments arranged by size: 3507 1834 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4265 4439 65 Size: 1183 3017 174 1396 Fragments arranged by size: 3017 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2513 4414 4601 1700 Size: 110 703 1901 187 2869 Fragments arranged by size: 2869 1901 703 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5770 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5770 BssSI C'ACGA_G Cuts at: 3830 4842 3830 Size: 1012 4758 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5770 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 2113 1184 Size: 929 4841 BstXI CCAn_nnnn'nTGG Cuts at: 2110 2593 2110 Size: 483 5287 BstYI r'GATC_y Cuts at: 1972 2685 3100 3467 3852 3869 5321 5407 5419 Size: 713 415 367 385 17 1452 86 12 Cuts at: 5419 5765 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 713 415 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2331 513 Size: 552 939 327 3952 Fragments arranged by size: 3952 939 552 327 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5031 Fragments arranged by size: 5031 683 56 BtsI GCAGTG_nn' Cuts at: 3286 4080 4100 3286 Size: 794 20 4956 Fragments arranged by size: 4956 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5735 DraI TTT'AAA Cuts at: 3074 3074 Size: 5770 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4777 809 Size: 507 3461 1802 Fragments arranged by size: 3461 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2358 2376 Size: 54 1425 50 275 18 315 213 18 Cuts at: 2376 2928 3217 4106 5735 8 Size: 552 289 889 1629 43 Fragments arranged by size: 1629 1425 889 552 315 289 275 213 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3217 1537 Size: 275 1405 4090 Fragments arranged by size: 4090 1405 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 2198 3700 1353 Size: 737 108 1502 3423 Fragments arranged by size: 3423 1502 737 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4357 4731 4877 387 Size: 1064 138 2768 374 146 1280 Fragments arranged by size: 2768 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2450 2579 3051 3833 4430 5217 Size: 393 398 420 129 472 782 597 787 Cuts at: 5217 5485 1239 Size: 268 1524 Fragments arranged by size: 1524 787 782 597 472 420 398 393 268 129 EcoNI CCTnn'n_nnAGG Cuts at: 2941 2941 Size: 5770 EcoRI G'AATT_C Cuts at: 2036 3079 3457 2036 Size: 1043 378 4349 Fragments arranged by size: 4349 1043 378 FspI TGC'GCA Cuts at: 4276 4276 Size: 5770 HaeII r_GCGC'y Cuts at: 1678 4917 1678 Size: 3239 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4411 4443 4485 4517 1373 Size: 32 135 32 2839 32 42 32 2626 Fragments arranged by size: 2839 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2212 3114 3360 669 Size: 988 281 274 902 246 3079 Fragments arranged by size: 3079 988 902 281 274 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5770 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KpnI G_GTAC'C Cuts at: 3095 3095 Size: 5770 MfeI C'AATT_G Cuts at: 3369 3369 Size: 5770 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4884 5068 1305 Size: 3579 184 2007 Fragments arranged by size: 3579 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2147 2360 2378 10 Size: 54 1425 343 315 213 18 3402 Fragments arranged by size: 3402 1425 343 315 213 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2303 2618 2744 2852 3008 3728 Size: 648 1011 126 315 126 108 156 720 Cuts at: 3728 4087 4246 518 Size: 359 159 2042 Fragments arranged by size: 2042 1011 720 648 359 315 159 156 126 126 108 MspA1I CmG'CkG Cuts at: 1606 3186 3863 5011 5256 1606 Size: 1580 677 1148 245 2120 Fragments arranged by size: 2120 1580 1148 677 245 NaeI GCC'GGC Cuts at: 2515 2515 Size: 5770 NcoI C'CATG_G Cuts at: 513 1065 2004 2331 513 Size: 552 939 327 3952 Fragments arranged by size: 3952 939 552 327 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NgoMIV G'CCGG_C Cuts at: 2513 2513 Size: 5770 NheI G'CTAG_C Cuts at: 1052 2248 1052 Size: 1196 4574 NotI GC'GGCC_GC Cuts at: 3217 3217 Size: 5770 NsiI A_TGCA'T Cuts at: 3015 3015 Size: 5770 NspI r_CATG'y Cuts at: 1132 1229 3128 4673 1132 Size: 97 1899 1545 2229 Fragments arranged by size: 2229 1899 1545 97 PasI CC'CwG_GG Cuts at: 1348 2905 1348 Size: 1557 4213 PciI A'CATG_T Cuts at: 4669 4669 Size: 5770 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5770 PfoI T'CCnGG_A Cuts at: 2977 2977 Size: 5770 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5770 PsiI TTA'TAA Cuts at: 3340 3340 Size: 5770 PstI C_TGCA'G Cuts at: 830 3122 830 Size: 2292 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5770 SalI G'TCGA_C Cuts at: 1936 3112 1936 Size: 1176 4594 SbfI CC_TGCA'GG Cuts at: 3122 3122 Size: 5770 ScaI AGT'ACT Cuts at: 4018 5730 4018 Size: 1712 4058 SexAI A'CCwGG_T Cuts at: 2109 2109 Size: 5770 SfcI C'TryA_G Cuts at: 826 1045 2872 3118 4253 4934 5125 826 Size: 219 1827 246 1135 681 191 1471 Fragments arranged by size: 1827 1471 1135 681 246 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 3097 1872 Size: 1225 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3874 4775 5037 820 Size: 197 926 1931 901 262 1553 Fragments arranged by size: 1931 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3561 493 Size: 3068 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 3128 1132 Size: 1996 3774 SspI AAT'ATT Cuts at: 5 52 3694 5 Size: 47 3642 2081 Fragments arranged by size: 3642 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5770 StyI C'CwwG_G Cuts at: 513 1065 2004 2331 2942 3209 513 Size: 552 939 327 611 267 3074 Fragments arranged by size: 3074 939 611 552 327 267 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2191 2315 3797 3814 3967 4152 4597 Size: 1255 611 124 1482 17 153 185 445 Cuts at: 4597 325 Size: 1498 Fragments arranged by size: 1498 1482 1255 611 445 185 153 124 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2778 4016 Size: 274 80 33 51 1443 204 597 1238 Cuts at: 4016 5728 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1238 597 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5730 5762 5730 Size: 32 5738 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5770 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2284 2284 Size: 5770 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 3095 1870 Size: 1225 4545 XmnI GAAnn'nnTTC Cuts at: 2714 3899 2714 Size: 1185 4585 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3578 276 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI