Restriction map for
pFN21AE0886
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI TfiI Van91I
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AocI 900 CCTGAGG CC^T[ACGT]AGG
AspI 838 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 602 CTCGAG C^[CT]CG[GA]G
BfaI 328 CTAG C^TAG
BsaBI 621 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 796 GGTCTC GGTCTC(1/5)
BsoBI 602 CTCGAG C^[CT]CG[GA]G
Bsp1407I 857 TGTACA T^GTACA
BspHI 431 TCATGA T^CATGA
BsrGI 857 TGTACA T^GTACA
BsrI 150 ACTGG ACTGG(1/-1)
BstAPI 246 GCAGAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 867 GGTGACC G^GT[ACGT]ACC
BstPI 867 GGTGACC G^GT[ACGT]ACC
BstXI 110 CCACCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 324 GGATCT [GA]^GATC[CT]
Bsu36I 900 CCTGAGG CC^T[ACGT]AGG
CfoI 463 GCGC GCG^C
Eco81I 900 CCTGAGG CC^T[ACGT]AGG
EcoO65I 867 GGTGACC G^GT[ACGT]ACC
HaeII 462 GGCGCT [GA]GCGC^[CT]
HhaI 463 GCGC GCG^C
HinP1I 463 GCGC G^CGC
HinfI 154 GACTC G^A[ACGT]TC
MaeI 328 CTAG C^TAG
MamI 621 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 324 GGATCT [GA]^GATC[CT]
MseI 608 TTAA T^TAA
NcoI 350 CCATGG C^CATGG
NspI 251 ACATGC [GA]CATG^[CT]
PaeR7I 602 CTCGAG C^TCGAG
PflFI 838 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 154 GACTC GAGTC(4/5)
PstI 244 CTGCAG CTGCA^G
RcaI 431 TCATGA T^CATGA
SfaNI 23 GCATC GCATC(5/9)
SspBI 857 TGTACA T^GTACA
StuI 734 AGGCCT AGG^CCT
Tru9I 608 TTAA T^TAA
Tth111I 838 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 327 TCTAGA T^CTAGA
XhoI 602 CTCGAG C^TCGAG
XhoII 324 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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AfaI 303 GTAC GT^AC
858 GTAC
AlwI 546 GATCC GGATC(4/5)
324 GGATC
BbvI 66 GCAGC GCAGC(8/12)
243 GCTGC
BseRI 127 CTCCTC GAGGAG(10/8)
693 CTCCTC
BsgI 415 CTGCAC GTGCAG(16/14)
533 GTGCAG
DpnII 325 GATC ^GATC
546 GATC
DraII 673 GGGTCCT [GA]G^G[ACGT]CC[CT]
707 AGGTCCC
DsaI 350 CCATGG C^C[GA][CT]GG
455 CCGTGG
EcoO109I 673 GGGTCCT [GA]G^G[ACGT]CC[CT]
707 AGGTCCC
EcoT14I 350 CCATGG C^C[AT][AT]GG
669 CCTTGG
Fnu4HI 66 GCAGC GC^[ACGT]GC
243 GCTGC
HphI 618 GGTGA GGTGA(8/7)
867 GGTGA
ItaI 66 GCAGC GC^[ACGT]GC
243 GCTGC
MboI 325 GATC ^GATC
546 GATC
MboII 308 TCTTC GAAGA(8/7)
715 TCTTC
MwoI 234 GCCCAAATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
246 GCAGAACATGC
NdeII 325 GATC ^GATC
546 GATC
PpuMI 673 GGGTCCT [GA]G^G[AT]CC[CT]
707 AGGTCCC
RsaI 303 GTAC GT^AC
858 GTAC
Sau3AI 325 GATC ^GATC
546 GATC
SfcI 244 CTGCAG C^T[GA][CT]AG
894 CTACAG
SmlI 602 CTCGAG C^T[CT][GA]AG
703 CTCAAG
StyI 350 CCATGG C^C[AT][AT]GG
669 CCTTGG
TaqI 147 TCGA T^CGA
603 TCGA
TseI 66 GCAGC G^C[AT]GC
243 GCTGC
Tsp509I 239 AATT ^AATT
687 AATT
TthHB8I 147 TCGA T^CGA
603 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI AvaII BalI BanII
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EaeI EcoRII
FokI HaeIII MaeIII MluNI MnlI
MscI MslI MvaI NlaIII NlaIV
Sau96I ScrFI Tsp45I TspRI