Restriction map for
pFN21AE0885
(Circular) (Six-base) MAPSORT of: pFN21AE0885.seq Check: 4161 from: 1 to: 5695
pFN21AE0885 5695 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2229 2444 2229
Size: 215 5480
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3038 1937
Size: 1101 4594
Acc65I G'GTAC_C
Cuts at: 3016 3016
Size: 5695
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3758 5142 5410 1632
Size: 2126 1384 268 1917
Fragments arranged by size:
2126 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 2598 4594 5571 1203
Size: 1395 1996 977 1327
Fragments arranged by size:
1996 1395 1327 977
AgeI A'CCGG_T
Cuts at: 4526 4526
Size: 5695
AhdI GACnn_n'nnGTC
Cuts at: 4424 4424
Size: 5695
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5695
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2300 2332 2300
Size: 32 5663
AlwNI CAG_nnn'CTG
Cuts at: 2137 3438 5010 2137
Size: 1301 1572 2822
Fragments arranged by size:
2822 1572 1301
ApaLI G'TGCA_C
Cuts at: 3752 4908 3752
Size: 1156 4539
ApoI r'AATT_y
Cuts at: 1493 3004 3230 3382 5491 1493
Size: 1511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1511 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 2571 3020 1870
Size: 73 628 449 4545
Fragments arranged by size:
4545 628 449 73
BamHI G'GATC_C
Cuts at: 3025 3392 3025
Size: 367 5328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3016 4471 618
Size: 325 217 627 1229 1455 1842
Fragments arranged by size:
1842 1455 1229 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2471 2478 2956 3014 1391
Size: 1080 7 478 58 4072
Fragments arranged by size:
4072 1080 478 58 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3886 3920 1903
Size: 34 1949 34 3678
Fragments arranged by size:
3678 1949 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5695
BfrBI ATG'CAT
Cuts at: 2947 2947
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2411 4306
Size: 107 122 71 631 605 337 402 1895
Cuts at: 4306 136
Size: 1525
Fragments arranged by size:
1895 1525 631 605 402 337 122 107
71
BglII A'GATC_T
Cuts at: 5690 5690
Size: 5695
BlpI GC'TnA_GC
Cuts at: 3112 3112
Size: 5695
Bme1580I G_kGCm'C
Cuts at: 1790 2014 3756 4912 1790
Size: 224 1742 1156 2573
Fragments arranged by size:
2573 1742 1156 224
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5695
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3426 4384 442
Size: 416 53 83 399 24 2009 958 1753
Fragments arranged by size:
2009 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4355 1239
Size: 3116 2579
Bpu10I CC'TnA_GC
Cuts at: 2121 2121
Size: 5695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3820 4685 4983 3820
Size: 865 298 4532
Fragments arranged by size:
4532 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2377 2806 2968 4358 882
Size: 617 878 429 162 1390 2219
Fragments arranged by size:
2219 1390 878 617 429 162
BsaAI yAC'GTr
Cuts at: 493 1204 2717 3486 493
Size: 711 1513 769 2702
Fragments arranged by size:
2702 1513 769 711
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3502 3884 4519 275
Size: 53 83 186 936 1969 382 635 1451
Fragments arranged by size:
1969 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4128 4526 4800 4947 5358 4128
Size: 398 274 147 411 4465
Fragments arranged by size:
4465 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5470
Fragments arranged by size:
5470 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 2519 2522 1186
Size: 1294 39 3 4359
Fragments arranged by size:
4359 1294 39 3
BseYI C'CCAG_C
Cuts at: 2176 4898 5394 2176
Size: 2722 496 2477
Fragments arranged by size:
2722 2477 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2207 1163
Size: 573 471 4651
Fragments arranged by size:
4651 573 471
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3145 3906 4934 1540
Size: 275 186 1144 761 1028 2301
Fragments arranged by size:
2301 1144 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 3756 3841 4912 2190
Size: 1566 85 1071 2973
Fragments arranged by size:
2973 1566 1071 85
BsmI GAATG_Cn'
Cuts at: 3206 3299 3206
Size: 93 5602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2190 2471 2478 2956 3014 3756
Size: 399 224 176 281 7 478 58 742
Cuts at: 3756 3841 4912 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 478 399 281 224 176
85 58 7
BspEI T'CCGG_A
Cuts at: 5358 5358
Size: 5695
BspHI T'CATG_A
Cuts at: 2697 3584 5314 2697
Size: 887 1730 3078
Fragments arranged by size:
3078 1730 887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 2444 2844 3050 844
Size: 1385 215 400 206 3489
Fragments arranged by size:
3489 1385 400 215 206
BsrBI CCG'CTC
Cuts at: 1394 1823 3582 1394
Size: 429 1759 3507
Fragments arranged by size:
3507 1759 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4190 4364 65
Size: 1183 2942 174 1396
Fragments arranged by size:
2942 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4339 4526 1700
Size: 110 2529 187 2869
Fragments arranged by size:
2869 2529 187 110
BsrGI T'GTAC_A
Cuts at: 96 2601 96
Size: 2505 3190
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5695
BssSI C'ACGA_G
Cuts at: 3755 4767 3755
Size: 1012 4683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5695
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 2380 2554 1184
Size: 1196 174 4325
Fragments arranged by size:
4325 1196 174
BstXI CCAn_nnnn'nTGG
Cuts at: 2164 2302 2164
Size: 138 5557
BstYI r'GATC_y
Cuts at: 1972 2106 2133 2577 3025 3392 3777 3794 5246
Size: 134 27 444 448 367 385 17 1452
Cuts at: 5246 5332 5344 5690 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 448 444 385 367 346 134
86 27 17 12
Bsu36I CC'TnA_GG
Cuts at: 2689 2930 2689
Size: 241 5454
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4956
Fragments arranged by size:
4956 683 56
BtsI GCAGTG_nn'
Cuts at: 3211 4005 4025 3211
Size: 794 20 4881
Fragments arranged by size:
4881 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5660
DraI TTT'AAA
Cuts at: 2999 2999
Size: 5695
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4702 809
Size: 507 3386 1802
Fragments arranged by size:
3386 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2247 2403
Size: 54 1425 50 275 18 369 48 156
Cuts at: 2403 2430 3142 4031 5660 8
Size: 27 712 889 1629 43
Fragments arranged by size:
1629 1425 889 712 369 275 156 54
50 48 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3142 1537
Size: 275 1330 4090
Fragments arranged by size:
4090 1330 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2276 2594 2956 3625 1353
Size: 923 318 362 669 3423
Fragments arranged by size:
3423 923 669 362 318
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4282 4656 4802 387
Size: 1064 138 2693 374 146 1280
Fragments arranged by size:
2693 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3758 4355 5142 5410 1239
Size: 393 2126 597 787 268 1524
Fragments arranged by size:
2126 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2983 2983
Size: 5695
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
FspI TGC'GCA
Cuts at: 4201 4201
Size: 5695
HaeII r_GCGC'y
Cuts at: 1678 4842 1678
Size: 3164 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4336 4368 4410 4442 1373
Size: 32 135 32 2764 32 42 32 2626
Fragments arranged by size:
2764 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3039 3285 669
Size: 988 281 1101 246 3079
Fragments arranged by size:
3079 1101 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5695
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KpnI G_GTAC'C
Cuts at: 3020 3020
Size: 5695
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2652 4809 4993 1305
Size: 1347 2157 184 2007
Fragments arranged by size:
2157 2007 1347 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 2249 2432 10
Size: 54 1425 343 369 48 183 3273
Fragments arranged by size:
3273 1425 369 343 183 54 48
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2462 2793 2882 3653 4012 4171
Size: 648 1065 231 331 89 771 359 159
Cuts at: 4171 518
Size: 2042
Fragments arranged by size:
2042 1065 771 648 359 331 231 159
89
MspA1I CmG'CkG
Cuts at: 1606 3111 3788 4936 5181 1606
Size: 1505 677 1148 245 2120
Fragments arranged by size:
2120 1505 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5143
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 3142 3142
Size: 5695
NsiI A_TGCA'T
Cuts at: 2949 2949
Size: 5695
NspI r_CATG'y
Cuts at: 1132 1229 2602 2625 2947 3053 4598 1132
Size: 97 1373 23 322 106 1545 2229
Fragments arranged by size:
2229 1545 1373 322 106 97 23
PasI CC'CwG_GG
Cuts at: 1348 2471 1348
Size: 1123 4572
PciI A'CATG_T
Cuts at: 2598 4594 2598
Size: 1996 3699
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5695
PfoI T'CCnGG_A
Cuts at: 2956 2956
Size: 5695
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5277 5309 3764
Size: 32 1481 32 4150
Fragments arranged by size:
4150 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5695
PsiI TTA'TAA
Cuts at: 3265 3265
Size: 5695
PstI C_TGCA'G
Cuts at: 830 2841 3047 830
Size: 2011 206 3478
Fragments arranged by size:
3478 2011 206
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
SalI G'TCGA_C
Cuts at: 1936 3037 1936
Size: 1101 4594
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SexAI A'CCwGG_T
Cuts at: 2163 2163
Size: 5695
SfcI C'TryA_G
Cuts at: 826 1045 2837 2923 3043 4178 4859 5050 826
Size: 219 1792 86 120 1135 681 191 1471
Fragments arranged by size:
1792 1471 1135 681 219 191 120 86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 3022 1872
Size: 1150 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2571 3799 4700 4962 820
Size: 197 926 628 1228 901 262 1553
Fragments arranged by size:
1553 1228 926 901 628 262 197
SnaBI TAC'GTA
Cuts at: 493 2717 3486 493
Size: 2224 769 2702
Fragments arranged by size:
2702 2224 769
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 3053 1132
Size: 1921 3774
SspI AAT'ATT
Cuts at: 5 52 3619 5
Size: 47 3567 2081
Fragments arranged by size:
3567 2081 47
StuI AGG'CCT
Cuts at: 1517 2989 1517
Size: 1472 4223
StyI C'CwwG_G
Cuts at: 513 1065 2376 3134 513
Size: 552 1311 758 3074
Fragments arranged by size:
3074 1311 758 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2648 2783 3722 3739 3892 4077 4522
Size: 1255 1068 135 939 17 153 185 445
Cuts at: 4522 325
Size: 1498
Fragments arranged by size:
1498 1255 1068 939 445 185 153 135
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 2601 3941
Size: 274 80 33 51 1443 258 366 1340
Cuts at: 3941 5653 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1340 366 274 258 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5655 5687 5655
Size: 32 5663
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XhoI C'TCGA_G
Cuts at: 1943 2571 1943
Size: 628 5067
XmaI C'CCGG_G
Cuts at: 1870 3020 1870
Size: 1150 4545
XmnI GAAnn'nnTTC
Cuts at: 2915 3824 2915
Size: 909 4786
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI