(Circular) (Six-base) MAPSORT of: pFN21AE0883.seq Check: 1348 from: 1 to: 5647 pFN21AE0883 5647 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2732 2732 Size: 5647 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2580 3457 278 Size: 53 83 186 936 1044 877 2468 Fragments arranged by size: 2468 1044 936 877 186 83 53 AccI GT'mk_AC Cuts at: 1937 2228 2990 1937 Size: 291 762 4594 Fragments arranged by size: 4594 762 291 Acc65I G'GTAC_C Cuts at: 2968 2968 Size: 5647 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2702 2891 3710 5094 5362 1632 Size: 1070 189 819 1384 268 1917 Fragments arranged by size: 1917 1384 1070 819 268 189 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 4546 5523 1203 Size: 3343 977 1327 Fragments arranged by size: 3343 1327 977 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 2821 4376 2821 Size: 1555 4092 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5647 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5647 AlwNI CAG_nnn'CTG Cuts at: 3390 4962 3390 Size: 1572 4075 ApaLI G'TGCA_C Cuts at: 2926 3704 4860 2926 Size: 778 1156 3713 Fragments arranged by size: 3713 1156 778 ApoI r'AATT_y Cuts at: 1493 2036 2956 3182 3334 5443 1493 Size: 543 920 226 152 2109 1697 Fragments arranged by size: 2109 1697 920 543 226 152 AseI AT'TA_AT Cuts at: 160 1032 4201 160 Size: 872 3169 1606 Fragments arranged by size: 3169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2972 1870 Size: 73 1029 4545 Fragments arranged by size: 4545 1029 73 BamHI G'GATC_C Cuts at: 2977 3344 2977 Size: 367 5280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2267 2968 4423 618 Size: 325 217 627 480 701 1455 1842 Fragments arranged by size: 1842 1455 701 627 480 325 217 BanII G_rGCy'C Cuts at: 1391 2966 1391 Size: 1575 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4676 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2752 2786 3838 3872 1903 Size: 34 815 34 1052 34 3678 Fragments arranged by size: 3678 1052 815 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2654 3539 4749 2654 Size: 885 1210 3552 Fragments arranged by size: 3552 1210 885 BclI T'GATC_A Cuts at: 1548 2595 1548 Size: 1047 4600 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4258 136 Size: 107 122 71 631 605 2586 1525 Fragments arranged by size: 2586 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5642 5642 Size: 5647 BlpI GC'TnA_GC Cuts at: 2062 3064 2062 Size: 1002 4645 Bme1580I G_kGCm'C Cuts at: 1790 2930 3708 4864 1790 Size: 1140 778 1156 2573 Fragments arranged by size: 2573 1156 1140 778 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5647 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3378 4336 442 Size: 416 53 83 399 24 1961 958 1753 Fragments arranged by size: 1961 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2835 2835 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4307 1239 Size: 3068 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2906 3772 4637 4935 2906 Size: 866 865 298 3618 Fragments arranged by size: 3618 866 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4310 882 Size: 617 2811 2219 Fragments arranged by size: 2811 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3438 493 Size: 711 2234 2702 Fragments arranged by size: 2702 2234 711 BsaBI GATnn'nnATC Cuts at: 2547 5641 2547 Size: 3094 2553 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2577 3454 3836 4471 Size: 53 83 186 936 1044 877 382 635 Cuts at: 4471 275 Size: 1451 Fragments arranged by size: 1451 1044 936 877 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4080 4478 4752 4899 5310 4080 Size: 398 274 147 411 4417 Fragments arranged by size: 4417 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5422 Fragments arranged by size: 5422 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5647 BseYI C'CCAG_C Cuts at: 2301 4850 5346 2301 Size: 2549 496 2602 Fragments arranged by size: 2602 2549 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5074 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3097 3858 4886 1540 Size: 275 186 1096 761 1028 2301 Fragments arranged by size: 2301 1096 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2094 2930 3708 3793 4864 2094 Size: 836 778 85 1071 2877 Fragments arranged by size: 2877 1071 836 778 85 BsmI GAATG_Cn' Cuts at: 3158 3251 3158 Size: 93 5554 BsmBI CGTCTCn'nnnn_ Cuts at: 2584 2584 Size: 5647 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2930 2966 3708 3793 4864 1391 Size: 399 304 836 36 742 85 1071 2174 Fragments arranged by size: 2174 1071 836 742 399 304 85 36 BspEI T'CCGG_A Cuts at: 5310 5310 Size: 5647 BspHI T'CATG_A Cuts at: 3536 5266 3536 Size: 1730 3917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2732 3002 844 Size: 1888 270 3489 Fragments arranged by size: 3489 1888 270 BsrBI CCG'CTC Cuts at: 1394 1823 3534 1394 Size: 429 1711 3507 Fragments arranged by size: 3507 1711 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2139 4142 4316 65 Size: 1183 891 2003 174 1396 Fragments arranged by size: 2003 1396 1183 891 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4291 4478 1700 Size: 110 2481 187 2869 Fragments arranged by size: 2869 2481 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5647 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5647 BssSI C'ACGA_G Cuts at: 3707 4719 3707 Size: 1012 4635 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5647 BstBI TT'CG_AA Cuts at: 4451 4451 Size: 5647 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5647 BstXI CCAn_nnnn'nTGG Cuts at: 2122 2122 Size: 5647 BstYI r'GATC_y Cuts at: 1972 2977 3344 3729 3746 5198 5284 5296 5642 Size: 1005 367 385 17 1452 86 12 346 Cuts at: 5642 1972 Size: 1977 Fragments arranged by size: 1977 1452 1005 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2879 2879 Size: 5647 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4156 Fragments arranged by size: 4156 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2755 487 Size: 683 56 1529 3379 Fragments arranged by size: 3379 1529 683 56 BtsI GCAGTG_nn' Cuts at: 3163 3957 3977 3163 Size: 794 20 4833 Fragments arranged by size: 4833 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2951 2951 Size: 5647 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4654 809 Size: 507 3338 1802 Fragments arranged by size: 3338 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3094 3983 5612 Size: 54 1425 50 275 18 1264 889 1629 Cuts at: 5612 8 Size: 43 Fragments arranged by size: 1629 1425 1264 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3094 1537 Size: 275 1282 4090 Fragments arranged by size: 4090 1282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2312 2891 3577 1353 Size: 959 579 686 3423 Fragments arranged by size: 3423 959 686 579 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4234 4608 4754 387 Size: 1064 138 2645 374 146 1280 Fragments arranged by size: 2645 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2702 2891 3710 4307 5094 5362 1239 Size: 393 1070 189 819 597 787 268 1524 Fragments arranged by size: 1524 1070 819 787 597 393 268 189 EcoRI G'AATT_C Cuts at: 2036 2956 3334 2036 Size: 920 378 4349 Fragments arranged by size: 4349 920 378 FspI TGC'GCA Cuts at: 4153 4153 Size: 5647 HaeII r_GCGC'y Cuts at: 1678 4794 1678 Size: 3116 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2807 2839 2845 2877 4288 Size: 32 135 32 1235 32 6 32 1411 Cuts at: 4288 4320 4362 4394 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1411 1235 135 42 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 2212 2229 2826 2991 3237 669 Size: 988 281 274 17 597 165 246 3079 Fragments arranged by size: 3079 988 597 281 274 246 165 17 HindIII A'AGCT_T Cuts at: 748 748 Size: 5647 HpaI GTT'AAC Cuts at: 3237 3237 Size: 5647 KpnI G_GTAC'C Cuts at: 2972 2972 Size: 5647 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 5523 5523 Size: 5647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4761 4945 1305 Size: 3456 184 2007 Fragments arranged by size: 3456 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3825 Fragments arranged by size: 3825 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3605 3964 4123 518 Size: 648 2439 359 159 2042 Fragments arranged by size: 2439 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2500 2730 3063 3740 4888 5133 1606 Size: 894 230 333 677 1148 245 2120 Fragments arranged by size: 2120 1148 894 677 333 245 230 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4156 Fragments arranged by size: 4156 939 552 NdeI CA'TA_TG Cuts at: 387 2362 2802 387 Size: 1975 440 3232 Fragments arranged by size: 3232 1975 440 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 2134 3005 4550 1132 Size: 97 905 871 1545 2229 Fragments arranged by size: 2229 1545 905 871 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5647 PciI A'CATG_T Cuts at: 4546 4546 Size: 5647 PflMI CCAn_nnn'nTGG Cuts at: 1108 2437 1108 Size: 1329 4318 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 5229 5261 3716 Size: 32 1481 32 4102 Fragments arranged by size: 4102 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2234 1613 Size: 621 5026 PsiI TTA'TAA Cuts at: 3217 3217 Size: 5647 PstI C_TGCA'G Cuts at: 830 2562 2729 2999 830 Size: 1732 167 270 3478 Fragments arranged by size: 3478 1732 270 167 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 PvuII CAG'CTG Cuts at: 2500 2730 2500 Size: 230 5417 SalI G'TCGA_C Cuts at: 1936 2227 2989 1936 Size: 291 762 4594 Fragments arranged by size: 4594 762 291 SapI GCTCTTCn'nnn_ Cuts at: 2312 2312 Size: 5647 SbfI CC_TGCA'GG Cuts at: 2999 2999 Size: 5647 ScaI AGT'ACT Cuts at: 3895 5607 3895 Size: 1712 3935 SfcI C'TryA_G Cuts at: 826 1045 2232 2558 2725 2995 4130 4811 5002 Size: 219 1187 326 167 270 1135 681 191 Cuts at: 5002 826 Size: 1471 Fragments arranged by size: 1471 1187 1135 681 326 270 219 191 167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2974 1872 Size: 1102 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2921 3751 4652 4914 820 Size: 197 926 978 830 901 262 1553 Fragments arranged by size: 1553 978 926 901 830 262 197 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5647 SphI G_CATG'C Cuts at: 1132 3005 1132 Size: 1873 3774 SspI AAT'ATT Cuts at: 5 52 3571 5 Size: 47 3519 2081 Fragments arranged by size: 3519 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5647 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 3086 513 Size: 552 939 117 965 3074 Fragments arranged by size: 3074 965 939 552 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2315 3674 3691 3844 4029 4474 325 Size: 1255 735 1359 17 153 185 445 1498 Fragments arranged by size: 1498 1359 1255 735 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3893 5605 Size: 274 80 33 51 1443 204 1712 1712 Cuts at: 5605 96 Size: 138 Fragments arranged by size: 1712 1712 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2156 2188 5607 5639 2156 Size: 32 3419 32 2164 Fragments arranged by size: 3419 2164 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5647 XbaI T'CTAG_A Cuts at: 2983 2983 Size: 5647 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2438 2438 Size: 5647 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5647 XmaI C'CCGG_G Cuts at: 1870 2972 1870 Size: 1102 4545 XmnI GAAnn'nnTTC Cuts at: 2864 3776 2864 Size: 912 4735 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2578 3455 276 Size: 53 83 186 936 1044 877 2468 Fragments arranged by size: 2468 1044 936 877 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI