Restriction map for
pFN21AE0882
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccII AccIII AcsI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I CelII CfoI ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AclI 529 AACGTT AA^CGTT
AfaI 661 GTAC GT^AC
Alw44I 914 GTGCAC G^TGCAC
ApaI 642 GGGCCC GGGCC^C
ApaLI 914 GTGCAC G^TGCAC
AspHI 914 GTGCAC G[AT]GC[AT]^C
AvaI 57 CTCGAG C^[CT]CG[GA]G
BamHI 742 GGATCC G^GATCC
BanI 775 GGCACC G^G[CT][GA]CC
BanII 642 GGGCCC G[GA]GC[CT]^C
BfaI 439 CTAG C^TAG
BpmI 162 CTCCAG CTGGAG(16/14)
BsaI 261 GGTCTC GGTCTC(1/5)
BsaWI 33 TCCGGT [AT]^CCGG[AT]
BseRI 82 CTCCTC GAGGAG(10/8)
BsiHKAI 914 GTGCAC G[AT]GC[AT]^C
BsoBI 57 CTCGAG C^[CT]CG[GA]G
BsrDI 348 CATTGC GCAATG(2/0)
BsrFI 560 ACCGGC [GA]^CCGG[CT]
Bst1107I 849 GTATAC GTA^TAC
BstXI 116 CCACCATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 742 GGATCC [GA]^GATC[CT]
BstZ17I 849 GTATAC GTA^TAC
Cfr10I 560 ACCGGC [GA]^CCGG[CT]
DraII 642 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 755 CCATGG C^C[GA][CT]GG
EarI 85 CTCTTC CTCTTC(1/4)
EcoO109I 642 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 320 ATGCAT ATGCA^T
FauI 290 CCCGC CCCGC(4/6)
HincII 532 GTTAAC GT[CT]^[GA]AC
HindII 532 GTTAAC GT[CT]^[GA]AC
HpaI 532 GTTAAC GTT^AAC
Ksp632I 85 CTCTTC CTCTTC(1/4)
MaeI 439 CTAG C^TAG
MflI 742 GGATCC [GA]^GATC[CT]
MseI 533 TTAA T^TAA
MslI 595 CATTCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 755 CCATGG C^CATGG
NsiI 320 ATGCAT ATGCA^T
PaeR7I 57 CTCGAG C^TCGAG
PflMI 243 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 529 AACGTT AA^CGTT
PstI 921 CTGCAG CTGCA^G
PvuII 707 CAGCTG CAG^CTG
RsaI 661 GTAC GT^AC
SfaNI 517 GATGC GCATC(5/9)
SfcI 921 CTGCAG C^T[GA][CT]AG
TaqI 58 TCGA T^CGA
TfiI 414 GAATC G^A[AT]TC
Tru9I 533 TTAA T^TAA
Tsp509I 678 AATT ^AATT
TthHB8I 58 TCGA T^CGA
Van91I 243 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 57 CTCGAG C^TCGAG
XhoII 742 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 244 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
707 CAGCTGCTG
BsmAI 262 GTCTC GTCTC(1/5)
730 GTCTC
BsmFI 158 GGGAC GGGAC(10/14)
486 GGGAC
BssKI 99 CCTGG ^CC[ACGT]GG
155 CCTGG
BstF5I 516 GGATG GGATG(2/0)
903 GGATG
BstNI 99 CCTGG CC^[AT]GG
155 CCTGG
DdeI 302 CTCAG C^T[ACGT]AG
818 CTCAG
DpnII 367 GATC ^GATC
743 GATC
EcoRII 99 CCTGG ^CC[AT]GG
155 CCTGG
EcoT14I 48 CCTTGG C^C[AT][AT]GG
755 CCATGG
FokI 516 GGATG GGATG(9/13)
903 GGATG
HapII 34 CCGG C^CGG
561 CCGG
HpaII 34 CCGG C^CGG
561 CCGG
MaeII 530 ACGT A^CGT
696 ACGT
MboI 367 GATC ^GATC
743 GATC
MspA1I 291 CCGCTG C[AC]G^C[GT]G
707 CAGCTG
MspI 34 CCGG C^CGG
561 CCGG
MvaI 99 CCTGG CC^[AT]GG
155 CCTGG
NdeII 367 GATC ^GATC
743 GATC
PleI 160 GACTC GAGTC(4/5)
488 GACTC
Sau3AI 367 GATC ^GATC
743 GATC
ScrFI 99 CCTGG CC^[ACGT]GG
155 CCTGG
SmlI 57 CTCGAG C^T[CT][GA]AG
444 CTTGAG
StyI 48 CCTTGG C^C[AT][AT]GG
755 CCATGG
TspRI 137 CACTG CA[GC]TG[ACGT][ACGT]^
236 CACTG
XcmI 473 CCATGTTGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
857 CCATTACACCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AccI AciI AluI AlwI BalI
BbvI BmyI BsaJI BsgI BsiYI
BslI Bsp1286I BsrI Cac8I Cfr13I
EaeI Fnu4HI HaeIII HinfI HphI
ItaI MaeIII MboII MluNI MnlI
MscI MwoI NlaIII NlaIV Sau96I
TseI Tsp45I