Restriction map for
pFN21AE0881
(Circular) (Six-base) MAPSORT of: pFN21AE0881.seq Check: 7203 from: 1 to: 5632
pFN21AE0881 5632 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2580 3442 278
Size: 53 83 186 936 1044 862 2468
Fragments arranged by size:
2468 1044 936 862 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 2896 3695 5079 5347 1632
Size: 947 317 799 1384 268 1917
Fragments arranged by size:
1917 1384 947 799 317 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 4531 5508 1203
Size: 3328 977 1327
Fragments arranged by size:
3328 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5632
AlwNI CAG_nnn'CTG
Cuts at: 3375 4947 3375
Size: 1572 4060
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2941 3167 3319 5428 1493
Size: 1448 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2957 1870
Size: 73 1014 4545
Fragments arranged by size:
4545 1014 73
AvrII C'CTAG_G
Cuts at: 2605 2605
Size: 5632
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2953 4408 618
Size: 325 217 627 1166 1455 1842
Fragments arranged by size:
1842 1455 1166 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2951 1391
Size: 1560 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2074 2668 928
Size: 971 175 594 3892
Fragments arranged by size:
3892 971 594 175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3823 3857 1903
Size: 34 1886 34 3678
Fragments arranged by size:
3678 1886 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2438 3524 4734 2438
Size: 1086 1210 3336
Fragments arranged by size:
3336 1210 1086
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BfrBI ATG'CAT
Cuts at: 2640 2640
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4243 136
Size: 107 122 71 631 605 2571 1525
Fragments arranged by size:
2571 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5627 5627
Size: 5632
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 3693 4849 1790
Size: 1903 1156 2573
Fragments arranged by size:
2573 1903 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3363 4321 442
Size: 416 53 83 399 24 1946 958 1753
Fragments arranged by size:
1946 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4292 1239
Size: 3053 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3757 4622 4920 3757
Size: 865 298 4469
Fragments arranged by size:
4469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4295 882
Size: 617 2796 2219
Fragments arranged by size:
2796 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3423 493
Size: 711 2219 2702
Fragments arranged by size:
2702 2219 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2577 3439 3821 4456
Size: 53 83 186 936 1044 862 382 635
Cuts at: 4456 275
Size: 1451
Fragments arranged by size:
1451 1044 936 862 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5407
Fragments arranged by size:
5407 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2917 1186
Size: 1731 3901
BseYI C'CCAG_C
Cuts at: 2441 2845 4835 5331 2441
Size: 404 1990 496 2742
Fragments arranged by size:
2742 1990 496 404
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2701 1163
Size: 573 965 4094
Fragments arranged by size:
4094 965 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2368 3082 3843 4871 1540
Size: 275 186 367 714 761 1028 2301
Fragments arranged by size:
2301 1028 761 714 367 275 186
BsiHKAI G_wGCw'C
Cuts at: 2473 3693 3778 4849 2473
Size: 1220 85 1071 3256
Fragments arranged by size:
3256 1220 1071 85
BsmI GAATG_Cn'
Cuts at: 3143 3236 3143
Size: 93 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2473 2951 3693 3778 4849 1391
Size: 399 683 478 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 683 478 399 85
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 2313 3521 5251 2313
Size: 1208 1730 2694
Fragments arranged by size:
2694 1730 1208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2987 844
Size: 2143 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3519 1394
Size: 429 1696 3507
Fragments arranged by size:
3507 1696 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2361 4127 4301 65
Size: 1183 1113 1766 174 1396
Fragments arranged by size:
1766 1396 1183 1113 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4276 4463 1700
Size: 110 2466 187 2869
Fragments arranged by size:
2869 2466 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 3692 4704 3692
Size: 1012 4620
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5632
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2962 3329 3714 3731 5183 5269 5281 5627
Size: 990 367 385 17 1452 86 12 346
Cuts at: 5627 1972
Size: 1977
Fragments arranged by size:
1977 1452 990 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2241 2241
Size: 5632
BtgI C'CryG_G
Cuts at: 513 1065 2417 513
Size: 552 1352 3728
Fragments arranged by size:
3728 1352 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 2820 2844 3148 3942 3962 2820
Size: 24 304 794 20 4490
Fragments arranged by size:
4490 794 304 24 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4639 809
Size: 507 3323 1802
Fragments arranged by size:
3323 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2250 2325
Size: 54 1425 50 275 18 315 105 75
Cuts at: 2325 2376 3079 3968 5597 8
Size: 51 703 889 1629 43
Fragments arranged by size:
1629 1425 889 703 315 275 105 75
54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3079 1537
Size: 275 1267 4090
Fragments arranged by size:
4090 1267 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2915 3562 1353
Size: 1562 647 3423
Fragments arranged by size:
3423 1562 647
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2771 4219 4593 4739 387
Size: 1064 138 1182 1448 374 146 1280
Fragments arranged by size:
1448 1280 1182 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2579 2896 3695 4292 5079 5347 1239
Size: 393 947 317 799 597 787 268 1524
Fragments arranged by size:
1524 947 799 787 597 393 317 268
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 4779 1678
Size: 3101 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2113 2145 4273 4305 4347
Size: 32 135 32 541 32 2128 32 42
Cuts at: 4347 4379 1373
Size: 32 2626
Fragments arranged by size:
2626 2128 541 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2212 2976 3222 669
Size: 988 281 274 764 246 3079
Fragments arranged by size:
3079 988 764 281 274 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4746 4930 1305
Size: 3441 184 2007
Fragments arranged by size:
3441 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 2252 2327 2378 10
Size: 54 1425 343 315 105 75 51 3264
Fragments arranged by size:
3264 1425 343 315 105 75 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 2586 2635 3590 3949 4108 518
Size: 648 1420 49 955 359 159 2042
Fragments arranged by size:
2042 1420 955 648 359 159 49
MspA1I CmG'CkG
Cuts at: 1606 2385 3048 3725 4873 5118 1606
Size: 779 663 677 1148 245 2120
Fragments arranged by size:
2120 1148 779 677 663 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5080
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NsiI A_TGCA'T
Cuts at: 2642 2642
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2776 2990 4535 1132
Size: 97 1547 214 1545 2229
Fragments arranged by size:
2229 1547 1545 214 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5632
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2263 1108
Size: 1155 4477
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2984 830
Size: 2154 3478
PvuI CG_AT'CG
Cuts at: 2001 2368 2001
Size: 367 5265
PvuII CAG'CTG
Cuts at: 2385 2385
Size: 5632
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 3880 5592 3880
Size: 1712 3920
SfcI C'TryA_G
Cuts at: 826 1045 2866 2900 2980 4115 4796 4987 826
Size: 219 1821 34 80 1135 681 191 1471
Fragments arranged by size:
1821 1471 1135 681 219 191 80 34
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5632
SmaI CCC'GGG
Cuts at: 1872 2959 1872
Size: 1087 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3736 4637 4899 820
Size: 197 926 1793 901 262 1553
Fragments arranged by size:
1793 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 2057 152
Size: 1905 3727
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5632
StyI C'CwwG_G
Cuts at: 513 1065 2205 2322 2328 2346 2605 3071 513
Size: 552 1140 117 6 18 259 466 3074
Fragments arranged by size:
3074 1140 552 466 259 117 18 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2440 3659 3676 3829 4014 4459 325
Size: 1255 860 1219 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1219 860 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3878 5590 96
Size: 274 80 33 51 1443 1901 1712 138
Fragments arranged by size:
1901 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5592 5624 5592
Size: 32 5600
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2260 2203
Size: 57 5575
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2957 1870
Size: 1087 4545
XmnI GAAnn'nnTTC
Cuts at: 2909 3761 2909
Size: 852 4780
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2578 3440 276
Size: 53 83 186 936 1044 862 2468
Fragments arranged by size:
2468 1044 936 862 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI