Restriction map for
pFN21AE0874
(Linear) (Six-base) MAPSORT of: KIEE0874.seq Check: 4330 from: 1 to: 954
KIEE0874.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 954
Size: 61 893
AflIII A'CryG_T
Cuts at: 0 653 954
Size: 653 301
AleI CACnn'nnGTG
Cuts at: 0 244 954
Size: 244 710
ApoI r'AATT_y
Cuts at: 0 31 539 954
Size: 31 508 415
Fragments arranged by size:
508 415 31
AseI AT'TA_AT
Cuts at: 0 637 954
Size: 637 317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 954
Size: 88 866
BseYI C'CCAG_C
Cuts at: 0 436 954
Size: 436 518
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 316 954
Size: 311 5 638
Fragments arranged by size:
638 311 5
BsiHKAI G_wGCw'C
Cuts at: 0 89 954
Size: 89 865
Bsp1286I G_dGCh'C
Cuts at: 0 89 954
Size: 89 865
BspHI T'CATG_A
Cuts at: 0 404 551 954
Size: 404 147 403
Fragments arranged by size:
404 403 147
BsrGI T'GTAC_A
Cuts at: 0 773 954
Size: 773 181
BstEII G'GTnAC_C
Cuts at: 0 55 954
Size: 55 899
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 954
Size: 27 927
BtgI C'CryG_G
Cuts at: 0 317 428 954
Size: 317 111 526
Fragments arranged by size:
526 317 111
BtsI GCAGTG_nn'
Cuts at: 0 299 954
Size: 299 655
DrdI GACnn_nn'nnGTC
Cuts at: 0 53 954
Size: 53 901
EaeI y'GGCC_r
Cuts at: 0 314 602 954
Size: 314 288 352
Fragments arranged by size:
352 314 288
EarI CTCTTCn'nnn_
Cuts at: 0 693 772 954
Size: 693 79 182
Fragments arranged by size:
693 182 79
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 954
Size: 61 893
EcoO109I rG'GnC_Cy
Cuts at: 0 440 954
Size: 440 514
EcoRV GAT'ATC
Cuts at: 0 394 954
Size: 394 560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 725 817 954
Size: 725 92 137
Fragments arranged by size:
725 137 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 954
Size: 383 32 539
Fragments arranged by size:
539 383 32
HindIII A'AGCT_T
Cuts at: 0 707 954
Size: 707 247
MfeI C'AATT_G
Cuts at: 0 493 954
Size: 493 461
MscI TGG'CCA
Cuts at: 0 316 604 954
Size: 316 288 350
Fragments arranged by size:
350 316 288
MslI CAynn'nnrTG
Cuts at: 0 25 172 244 403 598 775 954
Size: 25 147 72 159 195 177 179
Fragments arranged by size:
195 179 177 159 147 72 25
MspA1I CmG'CkG
Cuts at: 0 436 954
Size: 436 518
NcoI C'CATG_G
Cuts at: 0 317 428 954
Size: 317 111 526
Fragments arranged by size:
526 317 111
PfoI T'CCnGG_A
Cuts at: 0 442 954
Size: 442 512
PpuMI rG'GwC_Cy
Cuts at: 0 440 954
Size: 440 514
PshAI GACnn'nnGTC
Cuts at: 0 739 954
Size: 739 215
PstI C_TGCA'G
Cuts at: 0 471 508 954
Size: 471 37 446
Fragments arranged by size:
471 446 37
PvuII CAG'CTG
Cuts at: 0 436 954
Size: 436 518
SapI GCTCTTCn'nnn_
Cuts at: 0 693 954
Size: 693 261
SfcI C'TryA_G
Cuts at: 0 467 504 885 954
Size: 467 37 381 69
Fragments arranged by size:
467 381 69 37
SmlI C'TyrA_G
Cuts at: 0 67 954
Size: 67 887
SspI AAT'ATT
Cuts at: 0 749 954
Size: 749 205
StyI C'CwwG_G
Cuts at: 0 317 428 954
Size: 317 111 526
Fragments arranged by size:
526 317 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 74 954
Size: 74 880
TatI w'GTAC_w
Cuts at: 0 176 471 773 954
Size: 176 295 302 181
Fragments arranged by size:
302 295 181 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 216 248 954
Size: 216 32 706
Fragments arranged by size:
706 216 32
XmnI GAAnn'nnTTC
Cuts at: 0 35 954
Size: 35 919
Enzymes that do cut:
AcuI AflIII AleI ApoI AseI BpuEI BseYI BsgI
BsiHKAI Bsp1286I BspHI BsrGI BstEII BstXI BtgI BtsI
DrdI EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I
HindIII MfeI MscI MslI MspA1I NcoI PfoI PpuMI
PshAI PstI PvuII SapI SfcI SmlI SspI StyI
TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI