Restriction map for
pFN21AE0873
(Linear) (Six-base) MAPSORT of: KIEE0873.seq Check: 3552 from: 1 to: 966
KIEE0873.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AflIII A'CryG_T
Cuts at: 0 179 218 718 966
Size: 179 39 500 248
Fragments arranged by size:
500 248 179 39
AleI CACnn'nnGTG
Cuts at: 0 142 229 586 966
Size: 142 87 357 380
Fragments arranged by size:
380 357 142 87
AlwNI CAG_nnn'CTG
Cuts at: 0 75 156 966
Size: 75 81 810
Fragments arranged by size:
810 81 75
ApaI G_GGCC'C
Cuts at: 0 512 650 966
Size: 512 138 316
Fragments arranged by size:
512 316 138
ApaLI G'TGCA_C
Cuts at: 0 447 966
Size: 447 519
BanII G_rGCy'C
Cuts at: 0 8 419 512 650 966
Size: 8 411 93 138 316
Fragments arranged by size:
411 316 138 93 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 819 966
Size: 819 147
BbvCI CC'TCA_GC
Cuts at: 0 319 966
Size: 319 647
BfrBI ATG'CAT
Cuts at: 0 408 966
Size: 408 558
BglI GCCn_nnn'nGGC
Cuts at: 0 292 352 966
Size: 292 60 614
Fragments arranged by size:
614 292 60
Bme1580I G_kGCm'C
Cuts at: 0 236 451 512 650 966
Size: 236 215 61 138 316
Fragments arranged by size:
316 236 215 138 61
BmgBI CAC'GTC
Cuts at: 0 221 966
Size: 221 745
BmrI ACTGGGnnnn_n'
Cuts at: 0 652 779 853 966
Size: 652 127 74 113
Fragments arranged by size:
652 127 113 74
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 226 966
Size: 172 54 740
Fragments arranged by size:
740 172 54
Bpu10I CC'TnA_GC
Cuts at: 0 319 966
Size: 319 647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 894 966
Size: 894 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 444 966
Size: 444 522
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 930 960 966
Size: 199 30 701 30 6
Fragments arranged by size:
701 199 30 30 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 966
Size: 148 818
BsiHKAI G_wGCw'C
Cuts at: 0 451 966
Size: 451 515
Bsp1286I G_dGCh'C
Cuts at: 0 8 236 419 451 512 650 966
Size: 8 228 183 32 61 138 316
Fragments arranged by size:
316 228 183 138 61 32 8
BspHI T'CATG_A
Cuts at: 0 911 966
Size: 911 55
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 260 966
Size: 260 706
BsrGI T'GTAC_A
Cuts at: 0 176 966
Size: 176 790
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 966
Size: 75 891
BstEII G'GTnAC_C
Cuts at: 0 588 966
Size: 588 378
BstXI CCAn_nnnn'nTGG
Cuts at: 0 432 966
Size: 432 534
BtgI C'CryG_G
Cuts at: 0 140 966
Size: 140 826
BtsI GCAGTG_nn'
Cuts at: 0 847 966
Size: 847 119
DraIII CAC_nnn'GTG
Cuts at: 0 332 966
Size: 332 634
EaeI y'GGCC_r
Cuts at: 0 371 696 966
Size: 371 325 270
Fragments arranged by size:
371 325 270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 226 966
Size: 172 54 740
Fragments arranged by size:
740 172 54
EcoO109I rG'GnC_Cy
Cuts at: 0 72 153 646 647 966
Size: 72 81 493 1 319
Fragments arranged by size:
493 319 81 72 1
HaeII r_GCGC'y
Cuts at: 0 326 966
Size: 326 640
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 563 595 966
Size: 563 32 371
Fragments arranged by size:
563 371 32
MfeI C'AATT_G
Cuts at: 0 608 966
Size: 608 358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 966
Size: 476 490
MscI TGG'CCA
Cuts at: 0 373 966
Size: 373 593
MslI CAynn'nnrTG
Cuts at: 0 139 142 229 586 775 966
Size: 139 3 87 357 189 191
Fragments arranged by size:
357 191 189 139 87 3
MspA1I CmG'CkG
Cuts at: 0 39 966
Size: 39 927
NsiI A_TGCA'T
Cuts at: 0 410 966
Size: 410 556
NspI r_CATG'y
Cuts at: 0 183 722 966
Size: 183 539 244
Fragments arranged by size:
539 244 183
PciI A'CATG_T
Cuts at: 0 179 718 966
Size: 179 539 248
Fragments arranged by size:
539 248 179
PspOMI G'GGCC_C
Cuts at: 0 508 646 966
Size: 508 138 320
Fragments arranged by size:
508 320 138
PstI C_TGCA'G
Cuts at: 0 382 966
Size: 382 584
PvuII CAG'CTG
Cuts at: 0 39 966
Size: 39 927
SexAI A'CCwGG_T
Cuts at: 0 424 966
Size: 424 542
SfcI C'TryA_G
Cuts at: 0 378 867 966
Size: 378 489 99
Fragments arranged by size:
489 378 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 352 966
Size: 352 614
SmlI C'TyrA_G
Cuts at: 0 873 966
Size: 873 93
TatI w'GTAC_w
Cuts at: 0 176 940 966
Size: 176 764 26
Fragments arranged by size:
764 176 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 432 966
Size: 432 534
XmnI GAAnn'nnTTC
Cuts at: 0 684 966
Size: 684 282
Enzymes that do cut:
AflIII AleI AlwNI ApaI ApaLI BanII BbsI BbvCI
BfrBI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaXI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrGI
BstAPI BstEII BstXI BtgI BtsI DraIII EaeI Eco57MI
EcoO109I HaeII Hin4I MfeI MmeI MscI MslI MspA1I
NsiI NspI PciI PspOMI PstI PvuII SexAI SfcI
SfiI SmlI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BglII BlpI BmtI BplI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrFI BssHII BssSI BstBI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII TstI Tth111I XbaI XhoI XmaI ZraI