Restriction map for
pFN21AE0871
(Circular) (Six-base) MAPSORT of: pFN21AE0871.seq Check: 9273 from: 1 to: 5677
pFN21AE0871 5677 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2022 3020 1937
Size: 85 998 4594
Fragments arranged by size:
4594 998 85
Acc65I G'GTAC_C
Cuts at: 2998 2998
Size: 5677
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3740 5124 5392 1632
Size: 2108 1384 268 1917
Fragments arranged by size:
2108 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2026 820
Size: 197 1009 4471
Fragments arranged by size:
4471 1009 197
AflIII A'CryG_T
Cuts at: 1203 4576 5553 1203
Size: 3373 977 1327
Fragments arranged by size:
3373 1327 977
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2200 2448 1184
Size: 1016 248 4413
Fragments arranged by size:
4413 1016 248
AlwNI CAG_nnn'CTG
Cuts at: 3420 4992 3420
Size: 1572 4105
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2227 2961 2971 2986 3212 3364 5473 1493
Size: 734 734 10 15 226 152 2109 1697
Fragments arranged by size:
2109 1697 734 734 226 152 15 10
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 3002 1870
Size: 73 1059 4545
Fragments arranged by size:
4545 1059 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2411 2444 2411
Size: 33 5644
BamHI G'GATC_C
Cuts at: 2829 3007 3374 2829
Size: 178 367 5132
Fragments arranged by size:
5132 367 178
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2998 4453 618
Size: 325 217 627 1211 1455 1842
Fragments arranged by size:
1842 1455 1211 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2645 2996 1391
Size: 1254 351 4072
Fragments arranged by size:
4072 1254 351
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4706
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3868 3902 1903
Size: 34 1931 34 3678
Fragments arranged by size:
3678 1931 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2492 3569 4779 2492
Size: 1077 1210 3390
Fragments arranged by size:
3390 1210 1077
BclI T'GATC_A
Cuts at: 1548 2557 1548
Size: 1009 4668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4288 136
Size: 107 122 71 631 605 2616 1525
Fragments arranged by size:
2616 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2244 5672 2244
Size: 3428 2249
BlpI GC'TnA_GC
Cuts at: 3094 3094
Size: 5677
Bme1580I G_kGCm'C
Cuts at: 1790 2605 3738 4894 1790
Size: 815 1133 1156 2573
Fragments arranged by size:
2573 1156 1133 815
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5677
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2363 3408 4366
Size: 416 53 83 399 24 946 1045 958
Cuts at: 4366 442
Size: 1753
Fragments arranged by size:
1753 1045 958 946 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 4337 1239
Size: 1076 2022 2579
Fragments arranged by size:
2579 2022 1076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2261 3802 4667 4965 2261
Size: 1541 865 298 2973
Fragments arranged by size:
2973 1541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3468 493
Size: 711 2264 2702
Fragments arranged by size:
2702 2264 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3484 3866 4501 275
Size: 53 83 186 936 1951 382 635 1451
Fragments arranged by size:
1951 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4110 4508 4782 4929 5340 4110
Size: 398 274 147 411 4447
Fragments arranged by size:
4447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2276 2306 1178
Size: 30 165 30 873 30 4549
Fragments arranged by size:
4549 873 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2871 1186
Size: 1685 3992
BseYI C'CCAG_C
Cuts at: 2152 2221 2605 4880 5376 2152
Size: 69 384 2275 496 2453
Fragments arranged by size:
2453 2275 496 384 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 3888 4916 1540
Size: 275 186 1126 761 1028 2301
Fragments arranged by size:
2301 1126 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2645 3738 3823 4894 2645
Size: 1093 85 1071 3428
Fragments arranged by size:
3428 1093 1071 85
BsmI GAATG_Cn'
Cuts at: 3188 3281 3188
Size: 93 5584
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2605 2645 2996 3738 3823 4894 1391
Size: 399 815 40 351 742 85 1071 2174
Fragments arranged by size:
2174 1071 815 742 399 351 85 40
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 2439 3566 5296 2439
Size: 1127 1730 2820
Fragments arranged by size:
2820 1730 1127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3032 844
Size: 2188 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3564 1394
Size: 429 1741 3507
Fragments arranged by size:
3507 1741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4172 4346 65
Size: 1183 2924 174 1396
Fragments arranged by size:
2924 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4321 4508 1700
Size: 110 2511 187 2869
Fragments arranged by size:
2869 2511 187 110
BsrGI T'GTAC_A
Cuts at: 96 2670 96
Size: 2574 3103
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 3737 4749 3737
Size: 1012 4665
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5677
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 2244 2829 2850 3007 3374 3759 3776 5228
Size: 272 585 21 157 367 385 17 1452
Cuts at: 5228 5314 5326 5672 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 585 385 367 346 272 157
86 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4938
Fragments arranged by size:
4938 683 56
BtsI GCAGTG_nn'
Cuts at: 2184 2420 3193 3987 4007 2184
Size: 236 773 794 20 3854
Fragments arranged by size:
3854 794 773 236 20
ClaI AT'CG_AT
Cuts at: 1554 2398 1554
Size: 844 4833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DraIII CAC_nnn'GTG
Cuts at: 2059 2059
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2406 3124 4013
Size: 54 1425 50 275 18 576 718 889
Cuts at: 4013 5642 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 718 576 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3124 1537
Size: 275 1312 4090
Fragments arranged by size:
4090 1312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2120 2913 3607 1353
Size: 767 793 694 3423
Fragments arranged by size:
3423 793 767 694
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2643 2643
Size: 5677
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 3740 4337 5124 5392 1239
Size: 393 683 1425 597 787 268 1524
Fragments arranged by size:
1524 1425 787 683 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2716 2716
Size: 5677
EcoRI G'AATT_C
Cuts at: 2961 2986 3364 2961
Size: 25 378 5274
Fragments arranged by size:
5274 378 25
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 4824 1678
Size: 3146 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2154 2186 2673 2705 2890
Size: 32 135 32 582 32 487 32 185
Cuts at: 2890 2922 2950 2982 4318 4350 4392 4424 1373
Size: 32 28 32 1336 32 42 32 2626
Fragments arranged by size:
2626 1336 582 487 185 135 42 32
32 32 32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 2284 3021 3267 669
Size: 988 281 346 737 246 3079
Fragments arranged by size:
3079 988 737 346 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KpnI G_GTAC'C
Cuts at: 3002 3002
Size: 5677
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2714 4791 4975 1305
Size: 1409 2077 184 2007
Fragments arranged by size:
2077 2007 1409 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2408 10
Size: 54 1425 343 576 3279
Fragments arranged by size:
3279 1425 576 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 2666 2804 3635 3994 4153 518
Size: 648 1041 459 138 831 359 159 2042
Fragments arranged by size:
2042 1041 831 648 459 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2705 2729 3093 3770 4918 5163 1606
Size: 1099 24 364 677 1148 245 2120
Fragments arranged by size:
2120 1148 1099 677 364 245 24
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5125
NdeI CA'TA_TG
Cuts at: 387 2824 387
Size: 2437 3240
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 2278 3035 4580 1132
Size: 97 1049 757 1545 2229
Fragments arranged by size:
2229 1545 1049 757 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2716 2716
Size: 5677
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PstI C_TGCA'G
Cuts at: 830 2427 2728 3029 830
Size: 1597 301 301 3478
Fragments arranged by size:
3478 1597 301 301
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
PvuII CAG'CTG
Cuts at: 2705 2729 2705
Size: 24 5653
SacI G_AGCT'C
Cuts at: 2645 2645
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 3925 5637 3925
Size: 1712 3965
SfcI C'TryA_G
Cuts at: 826 1045 2423 2724 3025 4160 4841 5032 826
Size: 219 1378 301 301 1135 681 191 1471
Fragments arranged by size:
1471 1378 1135 681 301 301 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 3004 1872
Size: 1132 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2026 2240 3781 4682 4944 820
Size: 197 926 83 214 1541 901 262 1553
Fragments arranged by size:
1553 1541 926 901 262 214 197 83
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 2663 152
Size: 2511 3166
SphI G_CATG'C
Cuts at: 1132 3035 1132
Size: 1903 3774
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5677
StyI C'CwwG_G
Cuts at: 513 1065 2301 2494 3116 513
Size: 552 1236 193 622 3074
Fragments arranged by size:
3074 1236 622 552 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2310 2433 3704 3721 3874 4059 4504
Size: 1255 730 123 1271 17 153 185 445
Cuts at: 4504 325
Size: 1498
Fragments arranged by size:
1498 1271 1255 730 445 185 153 123
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 2670 3923
Size: 274 80 33 51 1443 234 459 1253
Cuts at: 3923 5635 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1253 459 274 234 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5637 5669 5637
Size: 32 5645
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 3002 1870
Size: 1132 4545
XmnI GAAnn'nnTTC
Cuts at: 3806 3806
Size: 5677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI