Restriction map for
pFN21AE0870
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BanI BanII BbeI
BbrPI BcgI BclI BcnI BfrI
BglII BlnI BsaAI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI SwaI TaqI
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AocI 777 CCTGAGG CC^T[ACGT]AGG
AspHI 380 GTGCTC G[AT]GC[AT]^C
AvaI 579 CCCGAG C^[CT]CG[GA]G
BalI 371 TGGCCA TGG^CCA
BamHI 680 GGATCC G^GATCC
BciVI 454 GGATAC GTATCC(6/5)
BglI 313 GCCTCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 552 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 552 GCTGAGC GC^T[ACGT]AGC
BsgI 163 CTGCAC GTGCAG(16/14)
BsiHKAI 380 GTGCTC G[AT]GC[AT]^C
BsmFI 263 GGGAC GGGAC(10/14)
BsoBI 579 CCCGAG C^[CT]CG[GA]G
BspMI 211 ACCTGC ACCTGC(4/8)
BsrDI 122 GCAATG GCAATG(2/0)
BstF5I 231 CATCC GGATG(2/0)
BstXI 143 CCATCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 777 CCTGAGG CC^T[ACGT]AGG
CelII 552 GCTGAGC GC^T[ACGT]AGC
DsaI 350 CCATGG C^C[GA][CT]GG
EaeI 371 TGGCCA [CT]^GGCC[GA]
EarI 181 CTCTTC CTCTTC(1/4)
Eco81I 777 CCTGAGG CC^T[ACGT]AGG
EcoNI 20 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 350 CCATGG C^C[AT][AT]GG
FokI 231 CATCC GGATG(9/13)
HinfI 408 GAATC G^A[ACGT]TC
HphI 641 TCACC GGTGA(8/7)
Ksp632I 181 CTCTTC CTCTTC(1/4)
MaeII 14 ACGT A^CGT
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MseI 854 TTAA T^TAA
MslI 168 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 427 CAGCTG C[AC]G^C[GT]G
NcoI 350 CCATGG C^CATGG
PstI 504 CTGCAG CTGCA^G
PvuII 427 CAGCTG CAG^CTG
SfaNI 227 GCATC GCATC(5/9)
SmlI 802 CTTGAG C^T[CT][GA]AG
SpeI 480 ACTAGT A^CTAGT
StuI 707 AGGCCT AGG^CCT
StyI 350 CCATGG C^C[AT][AT]GG
TfiI 408 GAATC G^A[AT]TC
Tru9I 854 TTAA T^TAA
TspRI 737 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 21 enzymes) #
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Asp700I 705 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
906 GAATGCTTTC
AvaII 264 GGACC G^G[AT]CC
615 GGTCC
BbsI 37 GTCTTC GAAGAC(2/6)
127 GTCTTC
BbvCI 588 GCTGAGG CCTCAGC(-5/-2)
691 GCTGAGG
BmyI 380 GTGCTC G[AGT]GC[ACT]^C
422 GTGCCC
BpmI 51 CTCCAG CTGGAG(16/14)
24 CTGGAG
BpuAI 37 GTCTTC GAAGAC(2/6)
127 GTCTTC
BsaBI 154 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
594 GATCTTTATC
BsaI 208 GAGACC GGTCTC(1/5)
624 GGTCTC
BsaJI 42 CCTGGG C^C[ACGT][ACGT]GG
350 CCATGG
BsmAI 208 GAGAC GTCTC(1/5)
625 GTCTC
BsmI 239 GAATGC GAATGC(1/-1)
906 GAATGC
Bsp1286I 380 GTGCTC G[AGT]GC[ACT]^C
422 GTGCCC
BstYI 593 GGATCT [GA]^GATC[CT]
680 GGATCC
Cac8I 397 GCCAGC GC[ACGT]^[ACGT]GC
790 GCCAGC
MamI 154 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
594 GATCTTTATC
MflI 593 GGATCT [GA]^GATC[CT]
680 GGATCC
NlaIV 263 GGGACC GG[ACGT]^[ACGT]CC
680 GGATCC
SfcI 504 CTGCAG C^T[GA][CT]AG
836 CTGTAG
XhoII 593 GGATCT [GA]^GATC[CT]
680 GGATCC
XmnI 705 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
906 GAATGCTTTC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI AlwI BbvI BfaI
BsiYI BslI BsrI BssKI BstNI
Cfr13I DdeI DpnII EcoRII Fnu4HI
HaeIII ItaI MaeI MaeIII MboI
MboII MnlI MvaI MwoI NdeII
NlaIII RsaI Sau3AI Sau96I ScrFI
TseI Tsp45I