Restriction map for
pFN21AE0870
(Linear) (Six-base) MAPSORT of: KIEE0870.seq Check: 414 from: 1 to: 939
KIEE0870.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AvaI C'yCGr_G
Cuts at: 0 579 939
Size: 579 360
BamHI G'GATC_C
Cuts at: 0 680 939
Size: 680 259
BbsI GAAGACnn'nnnn_
Cuts at: 0 30 120 939
Size: 30 90 819
Fragments arranged by size:
819 90 30
BbvCI CC'TCA_GC
Cuts at: 0 589 692 939
Size: 589 103 247
Fragments arranged by size:
589 247 103
BciVI GTATCCnnnnn_n'
Cuts at: 0 448 939
Size: 448 491
BglI GCCn_nnn'nGGC
Cuts at: 0 319 939
Size: 319 620
BlpI GC'TnA_GC
Cuts at: 0 553 939
Size: 553 386
Bme1580I G_kGCm'C
Cuts at: 0 426 939
Size: 426 513
BmrI ACTGGGnnnn_n'
Cuts at: 0 478 939
Size: 478 461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 45 939
Size: 36 9 894
Fragments arranged by size:
894 36 9
Bpu10I CC'TnA_GC
Cuts at: 0 589 692 939
Size: 589 103 247
Fragments arranged by size:
589 247 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 823 939
Size: 823 116
BsaI GGTCTCn'nnnn_
Cuts at: 0 202 630 939
Size: 202 428 309
Fragments arranged by size:
428 309 202
BsaBI GATnn'nnATC
Cuts at: 0 158 598 939
Size: 158 440 341
Fragments arranged by size:
440 341 158
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 939
Size: 4 30 905
Fragments arranged by size:
905 30 4
BseYI C'CCAG_C
Cuts at: 0 256 425 927 939
Size: 256 169 502 12
Fragments arranged by size:
502 256 169 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 939
Size: 148 791
BsiHKAI G_wGCw'C
Cuts at: 0 384 939
Size: 384 555
BsmI GAATG_Cn'
Cuts at: 0 245 912 939
Size: 245 667 27
Fragments arranged by size:
667 245 27
Bsp1286I G_dGCh'C
Cuts at: 0 384 426 939
Size: 384 42 513
Fragments arranged by size:
513 384 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 220 939
Size: 220 719
BsrDI GCAATG_nn'
Cuts at: 0 129 939
Size: 129 810
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 939
Size: 150 789
BstYI r'GATC_y
Cuts at: 0 593 680 939
Size: 593 87 259
Fragments arranged by size:
593 259 87
Bsu36I CC'TnA_GG
Cuts at: 0 778 939
Size: 778 161
BtgI C'CryG_G
Cuts at: 0 350 939
Size: 350 589
EaeI y'GGCC_r
Cuts at: 0 371 939
Size: 371 568
EarI CTCTTCn'nnn_
Cuts at: 0 187 939
Size: 187 752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 45 939
Size: 36 9 894
Fragments arranged by size:
894 36 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 24 939
Size: 24 915
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 726 939
Size: 726 213
MscI TGG'CCA
Cuts at: 0 373 939
Size: 373 566
MslI CAynn'nnrTG
Cuts at: 0 172 939
Size: 172 767
MspA1I CmG'CkG
Cuts at: 0 429 939
Size: 429 510
NcoI C'CATG_G
Cuts at: 0 350 939
Size: 350 589
PfoI T'CCnGG_A
Cuts at: 0 22 939
Size: 22 917
PstI C_TGCA'G
Cuts at: 0 508 939
Size: 508 431
PvuII CAG'CTG
Cuts at: 0 429 939
Size: 429 510
SfcI C'TryA_G
Cuts at: 0 504 836 939
Size: 504 332 103
Fragments arranged by size:
504 332 103
SmlI C'TyrA_G
Cuts at: 0 802 939
Size: 802 137
SpeI A'CTAG_T
Cuts at: 0 480 939
Size: 480 459
StuI AGG'CCT
Cuts at: 0 709 939
Size: 709 230
StyI C'CwwG_G
Cuts at: 0 350 939
Size: 350 589
TatI w'GTAC_w
Cuts at: 0 325 939
Size: 325 614
XmnI GAAnn'nnTTC
Cuts at: 0 709 910 939
Size: 709 201 29
Fragments arranged by size:
709 201 29
Enzymes that do cut:
AvaI BamHI BbsI BbvCI BciVI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BstXI BstYI
Bsu36I BtgI EaeI EarI Eco57MI EcoNI FalI MscI
MslI MspA1I NcoI PfoI PstI PvuII SfcI SmlI
SpeI StuI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI
BanII BbeI BcgI BclI BfrBI BglII BmgBI BmtI
BplI BsaAI BsaHI BsaWI BseRI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SspI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI ZraI