(Circular) (Six-base) MAPSORT of: pFN21AE0865.seq Check: 1408 from: 1 to: 5710 pFN21AE0865 5710 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2470 2792 2470 Size: 322 5388 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3053 1937 Size: 1116 4594 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2762 3773 5157 5425 1632 Size: 1130 1011 1384 268 1917 Fragments arranged by size: 1917 1384 1130 1011 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 4609 5586 1203 Size: 3406 977 1327 Fragments arranged by size: 3406 1327 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 2799 1166 Size: 1633 4077 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5710 AlwNI CAG_nnn'CTG Cuts at: 3453 5025 3453 Size: 1572 4138 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 2013 2096 2130 2545 3019 3245 3397 5506 Size: 520 83 34 415 474 226 152 2109 Cuts at: 5506 1493 Size: 1697 Fragments arranged by size: 2109 1697 520 474 415 226 152 83 34 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 3035 1870 Size: 73 1092 4545 Fragments arranged by size: 4545 1092 73 BamHI G'GATC_C Cuts at: 3040 3407 3040 Size: 367 5343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3031 4486 618 Size: 325 217 627 1244 1455 1842 Fragments arranged by size: 1842 1455 1244 627 325 217 BanII G_rGCy'C Cuts at: 1391 3029 1391 Size: 1638 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4739 BbvCI CC'TCA_GC Cuts at: 2752 2757 2752 Size: 5 5705 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3901 3935 1903 Size: 34 50 34 1880 34 3678 Fragments arranged by size: 3678 1880 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4321 136 Size: 107 122 71 631 605 2649 1525 Fragments arranged by size: 2649 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2064 5705 2064 Size: 3641 2069 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 3771 4927 1790 Size: 1981 1156 2573 Fragments arranged by size: 2573 1981 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3441 4399 442 Size: 416 53 83 399 24 2024 958 1753 Fragments arranged by size: 2024 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4370 1239 Size: 3131 2579 Bpu10I CC'TnA_GC Cuts at: 2752 2757 2752 Size: 5 5705 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3835 4700 4998 3835 Size: 865 298 4547 Fragments arranged by size: 4547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2267 4373 882 Size: 617 768 2106 2219 Fragments arranged by size: 2219 2106 768 617 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 2309 5704 2309 Size: 3395 2315 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3517 3899 4534 275 Size: 53 83 186 936 1984 382 635 1451 Fragments arranged by size: 1984 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4143 4541 4815 4962 5373 4143 Size: 398 274 147 411 4480 Fragments arranged by size: 4480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2664 2694 1178 Size: 30 165 30 1261 30 4194 Fragments arranged by size: 4194 1261 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5710 BseYI C'CCAG_C Cuts at: 2107 4913 5409 2107 Size: 2806 496 2408 Fragments arranged by size: 2806 2408 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5137 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3921 4949 1540 Size: 275 186 1159 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2359 3771 3856 4927 2359 Size: 1412 85 1071 3142 Fragments arranged by size: 3142 1412 1071 85 BsmI GAATG_Cn' Cuts at: 2256 2724 3221 3314 2256 Size: 468 497 93 4652 Fragments arranged by size: 4652 497 468 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2359 3029 3771 3856 4927 1391 Size: 399 569 670 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 670 569 399 85 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 3599 5329 3599 Size: 1730 3980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2470 2792 3065 844 Size: 1626 322 273 3489 Fragments arranged by size: 3489 1626 322 273 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2194 4205 4379 65 Size: 1183 946 2011 174 1396 Fragments arranged by size: 2011 1396 1183 946 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4354 4541 1700 Size: 110 2544 187 2869 Fragments arranged by size: 2869 2544 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5710 BssSI C'ACGA_G Cuts at: 3770 4782 3770 Size: 1012 4698 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 2089 1184 Size: 905 4805 BstYI r'GATC_y Cuts at: 1972 2064 3040 3407 3792 3809 5261 5347 5359 Size: 92 976 367 385 17 1452 86 12 Cuts at: 5359 5705 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 976 385 367 346 92 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2843 2843 Size: 5710 BtgI C'CryG_G Cuts at: 513 1065 2085 2925 513 Size: 552 1020 840 3298 Fragments arranged by size: 3298 1020 840 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4971 Fragments arranged by size: 4971 683 56 BtsI GCAGTG_nn' Cuts at: 3226 4020 4040 3226 Size: 794 20 4896 Fragments arranged by size: 4896 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2328 3157 4046 Size: 54 1425 50 275 18 498 829 889 Cuts at: 4046 5675 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 829 498 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2364 3640 1353 Size: 1011 1276 3423 Fragments arranged by size: 3423 1276 1011 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2762 3773 4370 5157 5425 1239 Size: 393 1130 1011 597 787 268 1524 Fragments arranged by size: 1524 1130 1011 787 597 393 268 EcoRI G'AATT_C Cuts at: 2096 3019 3397 2096 Size: 923 378 4409 Fragments arranged by size: 4409 923 378 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 2577 4857 1678 Size: 899 2280 2531 Fragments arranged by size: 2531 2280 899 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2893 2925 4351 4383 4425 Size: 32 135 32 1321 32 1426 32 42 Cuts at: 4425 4457 1373 Size: 32 2626 Fragments arranged by size: 2626 1426 1321 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2647 3054 3300 669 Size: 988 281 709 407 246 3079 Fragments arranged by size: 3079 988 709 407 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 2647 3300 2647 Size: 653 5057 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 2511 3309 2511 Size: 798 4912 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2892 2980 4824 5008 1305 Size: 1587 88 1844 184 2007 Fragments arranged by size: 2007 1844 1587 184 88 MscI TGG'CCA Cuts at: 10 64 1489 1832 2330 10 Size: 54 1425 343 498 3390 Fragments arranged by size: 3390 1425 498 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2237 2468 2799 3668 4027 4186 518 Size: 648 1071 231 331 869 359 159 2042 Fragments arranged by size: 2042 1071 869 648 359 331 231 159 MspA1I CmG'CkG Cuts at: 1606 2137 2501 2756 3126 3803 4951 5196 1606 Size: 531 364 255 370 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 531 370 364 255 245 NcoI C'CATG_G Cuts at: 513 1065 2085 2925 513 Size: 552 1020 840 3298 Fragments arranged by size: 3298 1020 840 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 3068 4613 1132 Size: 97 1839 1545 2229 Fragments arranged by size: 2229 1839 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5710 PciI A'CATG_T Cuts at: 4609 4609 Size: 5710 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5710 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2673 2705 2673 Size: 32 5678 PstI C_TGCA'G Cuts at: 830 3062 830 Size: 2232 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 PvuII CAG'CTG Cuts at: 2137 2501 2756 2137 Size: 364 255 5091 Fragments arranged by size: 5091 364 255 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SapI GCTCTTCn'nnn_ Cuts at: 2364 2364 Size: 5710 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 2850 3058 4193 4874 5065 826 Size: 219 1805 208 1135 681 191 1471 Fragments arranged by size: 1805 1471 1135 681 219 208 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 3037 1872 Size: 1165 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3814 4715 4977 820 Size: 197 926 1871 901 262 1553 Fragments arranged by size: 1871 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 3068 1132 Size: 1936 3774 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 2848 1517 Size: 1331 4379 StyI C'CwwG_G Cuts at: 513 1065 2085 2122 2251 2377 2925 3149 513 Size: 552 1020 37 129 126 548 224 3074 Fragments arranged by size: 3074 1020 552 548 224 129 126 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2399 3737 3754 3907 4092 4537 325 Size: 1255 819 1338 17 153 185 445 1498 Fragments arranged by size: 1498 1338 1255 819 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2241 2680 3956 Size: 274 80 33 51 1443 264 439 1276 Cuts at: 3956 5668 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1276 439 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5670 5702 5670 Size: 32 5678 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5710 XmaI C'CCGG_G Cuts at: 1870 3035 1870 Size: 1165 4545 XmnI GAAnn'nnTTC Cuts at: 3839 3839 Size: 5710 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI