(Circular) (Six-base) MAPSORT of: pFN21AE0863.seq Check: 8153 from: 1 to: 5644 pFN21AE0863 5644 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2735 2735 Size: 5644 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2106 2987 1937 Size: 169 881 4594 Fragments arranged by size: 4594 881 169 Acc65I G'GTAC_C Cuts at: 2444 2965 2444 Size: 521 5123 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3707 5091 5359 1632 Size: 2075 1384 268 1917 Fragments arranged by size: 2075 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 4543 5520 1203 Size: 3340 977 1327 Fragments arranged by size: 3340 1327 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5644 AlwNI CAG_nnn'CTG Cuts at: 3387 4959 3387 Size: 1572 4072 ApaI G_GGCC'C Cuts at: 2081 2908 2081 Size: 827 4817 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2036 2953 3179 3331 5440 1493 Size: 543 917 226 152 2109 1697 Fragments arranged by size: 2109 1697 917 543 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2969 1870 Size: 73 1026 4545 Fragments arranged by size: 4545 1026 73 BamHI G'GATC_C Cuts at: 2974 3341 2974 Size: 367 5277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2444 2675 2965 4420 618 Size: 325 217 627 657 231 290 1455 1842 Fragments arranged by size: 1842 1455 657 627 325 290 231 217 BanII G_rGCy'C Cuts at: 1391 2081 2217 2264 2420 2908 2963 1391 Size: 690 136 47 156 488 55 4072 Fragments arranged by size: 4072 690 488 156 136 55 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2308 2614 2710 928 Size: 971 409 306 96 3862 Fragments arranged by size: 3862 971 409 306 96 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3835 3869 1903 Size: 34 1898 34 3678 Fragments arranged by size: 3678 1898 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 2136 1548 Size: 588 5056 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2432 4255 136 Size: 107 122 71 631 605 760 1823 1525 Fragments arranged by size: 1823 1525 760 631 605 122 107 71 BglII A'GATC_T Cuts at: 2595 5639 2595 Size: 3044 2600 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 2081 2241 2908 3705 4861 1790 Size: 291 160 667 797 1156 2573 Fragments arranged by size: 2573 1156 797 667 291 160 BmgBI CAC'GTC Cuts at: 1318 2896 1318 Size: 1578 4066 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4304 1239 Size: 3065 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2048 2434 3769 4634 4932 2048 Size: 386 1335 865 298 2760 Fragments arranged by size: 2760 1335 865 386 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2755 4307 882 Size: 617 1256 1552 2219 Fragments arranged by size: 2219 1552 1256 617 BsaAI yAC'GTr Cuts at: 493 1204 3435 493 Size: 711 2231 2702 Fragments arranged by size: 2702 2231 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3451 3833 4468 275 Size: 53 83 186 936 1918 382 635 1451 Fragments arranged by size: 1918 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5419 Fragments arranged by size: 5419 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2722 2794 2914 1186 Size: 949 587 72 120 3916 Fragments arranged by size: 3916 949 587 120 72 BseYI C'CCAG_C Cuts at: 2009 2122 2576 4847 5343 2009 Size: 113 454 2271 496 2310 Fragments arranged by size: 2310 2271 496 454 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 2319 1163 Size: 573 417 166 4488 Fragments arranged by size: 4488 573 417 166 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2792 3094 3855 4883 1540 Size: 275 186 791 302 761 1028 2301 Fragments arranged by size: 2301 1028 791 761 302 275 186 BsiHKAI G_wGCw'C Cuts at: 2217 3705 3790 4861 2217 Size: 1488 85 1071 3000 Fragments arranged by size: 3000 1488 1071 85 BsmI GAATG_Cn' Cuts at: 3155 3248 3155 Size: 93 5551 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2217 2241 2264 2420 2908 2963 Size: 399 291 136 24 23 156 488 55 Cuts at: 2963 3705 3790 4861 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 488 399 291 156 136 85 55 24 23 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2735 2999 844 Size: 1891 264 3489 Fragments arranged by size: 3489 1891 264 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2694 4139 4313 65 Size: 1183 1446 1445 174 1396 Fragments arranged by size: 1446 1445 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4288 4475 1700 Size: 110 2478 187 2869 Fragments arranged by size: 2869 2478 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 2744 3704 4716 2744 Size: 960 1012 3672 Fragments arranged by size: 3672 1012 960 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 2113 1184 Size: 929 4715 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2248 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2595 2974 3341 3726 3743 5195 5281 5293 Size: 623 379 367 385 17 1452 86 12 Cuts at: 5293 5639 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 623 385 379 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2358 2420 2436 513 Size: 552 939 354 62 16 3721 Fragments arranged by size: 3721 939 552 354 62 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 2027 3160 3954 3974 2027 Size: 1133 794 20 3697 Fragments arranged by size: 3697 1133 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DraIII CAC_nnn'GTG Cuts at: 2920 2920 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2355 2433 Size: 54 1425 50 275 18 420 105 78 Cuts at: 2433 2472 2789 3091 3980 5609 8 Size: 39 317 302 889 1629 43 Fragments arranged by size: 1629 1425 889 420 317 302 275 105 78 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2789 3091 1537 Size: 275 977 302 4090 Fragments arranged by size: 4090 977 302 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 2762 2840 2918 3574 1353 Size: 869 540 78 78 656 3423 Fragments arranged by size: 3423 869 656 540 78 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2215 2215 Size: 5644 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3707 4304 5091 5359 1239 Size: 393 2075 597 787 268 1524 Fragments arranged by size: 2075 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2150 2150 Size: 5644 EcoO109I rG'GnC_Cy Cuts at: 2068 2077 2078 2244 2904 2905 2068 Size: 9 1 166 660 1 4807 Fragments arranged by size: 4807 660 166 9 1 1 EcoRI G'AATT_C Cuts at: 2036 2953 3331 2036 Size: 917 378 4349 Fragments arranged by size: 4349 917 378 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 2935 4791 1678 Size: 1257 1856 2531 Fragments arranged by size: 2531 1856 1257 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4285 4317 4359 4391 1373 Size: 32 135 32 2713 32 42 32 2626 Fragments arranged by size: 2713 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2988 3234 669 Size: 988 281 1050 246 3079 Fragments arranged by size: 3079 1050 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KpnI G_GTAC'C Cuts at: 2448 2969 2448 Size: 521 5123 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4758 4942 1305 Size: 3453 184 2007 Fragments arranged by size: 3453 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2252 2435 10 Size: 54 1425 343 420 183 3219 Fragments arranged by size: 3219 1425 420 343 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 2435 2855 3602 3961 4120 518 Size: 648 1191 78 420 747 359 159 2042 Fragments arranged by size: 2042 1191 747 648 420 359 159 78 MspA1I CmG'CkG Cuts at: 1606 3060 3737 4885 5130 1606 Size: 1454 677 1148 245 2120 Fragments arranged by size: 2120 1454 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2358 2436 513 Size: 552 939 354 78 3721 Fragments arranged by size: 3721 939 552 354 78 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 3002 4547 1132 Size: 97 1773 1545 2229 Fragments arranged by size: 2229 1773 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 4543 4543 Size: 5644 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2244 2244 Size: 5644 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PspOMI G'GGCC_C Cuts at: 2077 2904 2077 Size: 827 4817 PstI C_TGCA'G Cuts at: 830 2076 2996 830 Size: 1246 920 3478 Fragments arranged by size: 3478 1246 920 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 SacI G_AGCT'C Cuts at: 2217 2217 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SapI GCTCTTCn'nnn_ Cuts at: 2222 2222 Size: 5644 SbfI CC_TGCA'GG Cuts at: 2076 2996 2076 Size: 920 4724 ScaI AGT'ACT Cuts at: 2559 3892 5604 2559 Size: 1333 1712 2599 Fragments arranged by size: 2599 1712 1333 SexAI A'CCwGG_T Cuts at: 2109 2109 Size: 5644 SfcI C'TryA_G Cuts at: 826 1045 2072 2992 4127 4808 4999 826 Size: 219 1027 920 1135 681 191 1471 Fragments arranged by size: 1471 1135 1027 920 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2432 2432 Size: 5644 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2063 2413 3748 4649 4911 820 Size: 197 926 120 350 1335 901 262 1553 Fragments arranged by size: 1553 1335 926 901 350 262 197 120 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2004 2358 2436 3083 513 Size: 552 939 354 78 647 3074 Fragments arranged by size: 3074 939 647 552 354 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2080 2385 2495 2663 2714 2869 3671 Size: 1255 500 305 110 168 51 155 802 Cuts at: 3671 3688 3841 4026 4471 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 802 500 445 305 185 168 155 153 110 51 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2557 3890 Size: 274 80 33 51 1443 204 376 1333 Cuts at: 3890 5602 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1333 376 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 2822 1316 Size: 1506 4138 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2686 2686 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 3773 3773 Size: 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SfoI SrfI SwaI