Restriction map for
pFN21AE0856
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BcgI BcnI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MseI MspA1I MunI MvnI
NarI NciI NcoI NdeI NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI StuI SwaI TaqI Tru9I
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 31 GAATTT [GA]^AATT[CT]
AlwI 522 GATCC GGATC(4/5)
AlwNI 67 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 31 GAATTT [GA]^AATT[CT]
AvaII 443 GGACC G^G[AT]CC
BciVI 730 GTATCC GTATCC(6/5)
BclI 363 TGATCA T^GATCA
BglI 820 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 370 TACGTG [CT]AC^GT[GA]
BsmFI 413 GTCCC GGGAC(10/14)
BsmI 900 GAATGC GAATGC(1/-1)
Bsp1407I 809 TGTACA T^GTACA
BsrDI 116 CATTGC GCAATG(2/0)
BsrFI 704 GCCGGC [GA]^CCGG[CT]
BsrGI 809 TGTACA T^GTACA
BstAPI 66 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 704 GCCGGC [GA]^CCGG[CT]
DraII 794 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 367 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 417 CCGTGG C^C[GA][CT]GG
EcoO109I 794 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 791 CCAAGG C^C[AT][AT]GG
FbaI 363 TGATCA T^GATCA
HapII 705 CCGG C^CGG
HpaII 705 CCGG C^CGG
MaeII 371 ACGT A^CGT
MspI 705 CCGG C^CGG
NaeI 704 GCCGGC GCC^GGC
NgoMIV 704 GCCGGC G^CCGGC
SapI 258 GCTCTTC GCTCTTC(1/4)
SmlI 681 CTTGAG C^T[CT][GA]AG
SphI 242 GCATGC GCATG^C
SspBI 809 TGTACA T^GTACA
StyI 791 CCAAGG C^C[AT][AT]GG
Tsp509I 32 AATT ^AATT
XcmI 275 CCATCACCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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BalI 140 TGGCCA TGG^CCA
827 TGGCCA
BbsI 661 GTCTTC GAAGAC(2/6)
844 GTCTTC
BbvCI 858 CCTCAGC CCTCAGC(-5/-2)
697 GCTGAGG
BbvI 66 GCAGC GCAGC(8/12)
673 GCTGC
BmyI 194 GTGCCC G[AGT]GC[ACT]^C
425 GTGCCC
BpuAI 661 GTCTTC GAAGAC(2/6)
844 GTCTTC
Bsp1286I 194 GTGCCC G[AGT]GC[ACT]^C
425 GTGCCC
BstF5I 648 CATCC GGATG(2/0)
470 GGATG
EaeI 140 TGGCCA [CT]^GGCC[GA]
827 TGGCCA
EarI 184 CTCTTC CTCTTC(1/4)
259 CTCTTC
Fnu4HI 66 GCAGC GC^[ACGT]GC
673 GCTGC
FokI 648 CATCC GGATG(9/13)
470 GGATG
ItaI 66 GCAGC GC^[ACGT]GC
673 GCTGC
Ksp632I 184 CTCTTC CTCTTC(1/4)
259 CTCTTC
MluNI 140 TGGCCA TGG^CCA
827 TGGCCA
MscI 140 TGGCCA TGG^CCA
827 TGGCCA
NspI 242 GCATGC [GA]CATG^[CT]
401 ACATGC
PflMI 143 CCACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
429 CCATCTTGTGG
PleI 164 GACTC GAGTC(4/5)
411 GAGTC
SfaNI 603 GATGC GCATC(5/9)
817 GATGC
SfcI 15 CTACAG C^T[GA][CT]AG
759 CTATAG
TfiI 213 GATTC G^A[AT]TC
687 GATTC
TseI 66 GCAGC G^C[AT]GC
673 GCTGC
Van91I 143 CCACAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
429 CCATCTTGTGG
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI AluI BsaJI BsiYI BslI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII EcoRII HaeIII HinfI HphI
MaeIII MboI MboII MnlI MslI
MvaI MwoI NdeII NlaIII RsaI
Sau3AI Sau96I ScrFI Tsp45I TspRI