(Circular) (Six-base) MAPSORT of: pFN21AE0856.seq Check: 5710 from: 1 to: 5653 pFN21AE0856 5653 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2996 1937 Size: 1059 4594 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2921 3716 5100 5368 1632 Size: 1289 795 1384 268 1917 Fragments arranged by size: 1917 1384 1289 795 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 4552 5529 1203 Size: 3349 977 1327 Fragments arranged by size: 3349 1327 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 2288 2750 1166 Size: 1122 462 4069 Fragments arranged by size: 4069 1122 462 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5653 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2154 2186 2154 Size: 32 5621 AlwNI CAG_nnn'CTG Cuts at: 2077 3396 4968 2077 Size: 1319 1572 2762 Fragments arranged by size: 2762 1572 1319 ApaLI G'TGCA_C Cuts at: 3710 4866 3710 Size: 1156 4497 ApoI r'AATT_y Cuts at: 1493 2036 2962 3188 3340 5449 1493 Size: 543 926 226 152 2109 1697 Fragments arranged by size: 2109 1697 926 543 226 152 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2978 1870 Size: 73 1035 4545 Fragments arranged by size: 4545 1035 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2168 2201 2718 2751 2168 Size: 33 517 33 5070 Fragments arranged by size: 5070 517 33 33 BamHI G'GATC_C Cuts at: 2983 3350 2983 Size: 367 5286 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2974 4429 618 Size: 325 217 627 1187 1455 1842 Fragments arranged by size: 1842 1455 1187 627 325 217 BanII G_rGCy'C Cuts at: 1391 2972 1391 Size: 1581 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2659 2842 928 Size: 971 760 183 3739 Fragments arranged by size: 3739 971 760 183 BbvCI CC'TCA_GC Cuts at: 2703 2864 2703 Size: 161 5492 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3844 3878 1903 Size: 34 50 34 1823 34 3678 Fragments arranged by size: 3678 1823 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2746 3545 4755 2746 Size: 799 1210 3644 Fragments arranged by size: 3644 1210 799 BclI T'GATC_A Cuts at: 1548 2368 1548 Size: 820 4833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2831 4264 136 Size: 107 122 71 631 605 1159 1433 1525 Fragments arranged by size: 1525 1433 1159 631 605 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 3070 3070 Size: 5653 Bme1580I G_kGCm'C Cuts at: 1790 2203 2434 3714 4870 1790 Size: 413 231 1280 1156 2573 Fragments arranged by size: 2573 1280 1156 413 231 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5653 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3384 4342 442 Size: 416 53 83 399 24 1967 958 1753 Fragments arranged by size: 1967 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4313 1239 Size: 3074 2579 Bpu10I CC'TnA_GC Cuts at: 2703 2864 2703 Size: 161 5492 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2707 3778 4643 4941 2707 Size: 1071 865 298 3419 Fragments arranged by size: 3419 1071 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4316 882 Size: 617 2817 2219 Fragments arranged by size: 2817 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2377 3444 493 Size: 711 1173 1067 2702 Fragments arranged by size: 2702 1173 1067 711 BsaBI GATnn'nnATC Cuts at: 5647 5647 Size: 5653 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3460 3842 4477 275 Size: 53 83 186 936 1927 382 635 1451 Fragments arranged by size: 1927 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4086 4484 4758 4905 5316 4086 Size: 398 274 147 411 4423 Fragments arranged by size: 4423 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2154 2184 1178 Size: 30 165 30 751 30 4647 Fragments arranged by size: 4647 751 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5653 BseYI C'CCAG_C Cuts at: 2067 4856 5352 2067 Size: 2789 496 2368 Fragments arranged by size: 2789 2368 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5080 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 3864 4892 1540 Size: 275 186 1102 761 1028 2301 Fragments arranged by size: 2301 1102 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3714 3799 4870 3714 Size: 85 1071 4497 Fragments arranged by size: 4497 1071 85 BsmI GAATG_Cn' Cuts at: 2911 3164 3257 2911 Size: 253 93 5307 Fragments arranged by size: 5307 253 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2203 2434 2972 3714 3799 4870 1391 Size: 399 413 231 538 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 538 413 399 231 85 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3008 844 Size: 2164 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3540 1394 Size: 429 1717 3507 Fragments arranged by size: 3507 1717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2120 4148 4322 65 Size: 1183 872 2028 174 1396 Fragments arranged by size: 2028 1396 1183 872 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2709 4297 4484 1700 Size: 110 899 1588 187 2869 Fragments arranged by size: 2869 1588 899 187 110 BsrGI T'GTAC_A Cuts at: 96 2814 96 Size: 2718 2935 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 3713 4725 3713 Size: 1012 4641 BstAPI GCAn_nnn'nTGC Cuts at: 794 2077 794 Size: 1283 4370 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5653 BstYI r'GATC_y Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648 Size: 1011 367 385 17 1452 86 12 346 Cuts at: 5648 1972 Size: 1977 Fragments arranged by size: 1977 1452 1011 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2422 513 Size: 552 1357 3744 Fragments arranged by size: 3744 1357 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4914 Fragments arranged by size: 4914 683 56 BtsI GCAGTG_nn' Cuts at: 2393 3169 3963 3983 2393 Size: 776 794 20 4063 Fragments arranged by size: 4063 794 776 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DraIII CAC_nnn'GTG Cuts at: 2377 2377 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2832 3100 Size: 54 1425 50 275 18 315 687 268 Cuts at: 3100 3989 5618 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 687 315 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2195 2270 3583 1353 Size: 842 75 1313 3423 Fragments arranged by size: 3423 1313 842 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4240 4614 4760 387 Size: 1064 138 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2921 3716 4313 5100 5368 1239 Size: 393 1289 795 597 787 268 1524 Fragments arranged by size: 1524 1289 795 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2800 2800 Size: 5653 EcoRI G'AATT_C Cuts at: 2962 3340 2962 Size: 378 5275 FspI TGC'GCA Cuts at: 4159 4159 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 4800 1678 Size: 3122 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4294 4326 4368 4400 1373 Size: 32 135 32 2722 32 42 32 2626 Fragments arranged by size: 2722 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2997 3243 669 Size: 988 281 1059 246 3079 Fragments arranged by size: 3079 1059 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4767 4951 1305 Size: 3462 184 2007 Fragments arranged by size: 3462 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2147 2834 10 Size: 54 1425 343 315 687 2829 Fragments arranged by size: 2829 1425 687 343 315 54 MslI CAynn'nnrTG Cuts at: 518 1166 2180 2288 2306 2357 2411 2474 2603 Size: 648 1014 108 18 51 54 63 129 Cuts at: 2603 2750 3611 3970 4129 518 Size: 147 861 359 159 2042 Fragments arranged by size: 2042 1014 861 648 359 159 147 129 108 63 54 51 18 MspA1I CmG'CkG Cuts at: 1606 3069 3746 4894 5139 1606 Size: 1463 677 1148 245 2120 Fragments arranged by size: 2120 1463 1148 677 245 NaeI GCC'GGC Cuts at: 2711 2711 Size: 5653 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5101 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NgoMIV G'CCGG_C Cuts at: 2709 2709 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 2251 2410 3011 4556 1132 Size: 97 1022 159 601 1545 2229 Fragments arranged by size: 2229 1545 1022 601 159 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5653 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 2154 2440 1108 Size: 1046 286 4321 Fragments arranged by size: 4321 1046 286 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2154 2186 2207 2239 3722 3754 5235 5267 2154 Size: 32 21 32 1483 32 1481 32 2540 Fragments arranged by size: 2540 1483 1481 32 32 32 32 21 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PstI C_TGCA'G Cuts at: 830 3005 830 Size: 2175 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SapI GCTCTTCn'nnn_ Cuts at: 2270 2270 Size: 5653 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 3901 5613 3901 Size: 1712 3941 SfcI C'TryA_G Cuts at: 826 1045 2020 2764 3001 4136 4817 5008 826 Size: 219 975 744 237 1135 681 191 1471 Fragments arranged by size: 1471 1135 975 744 681 237 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2686 3757 4658 4920 820 Size: 197 926 743 1071 901 262 1553 Fragments arranged by size: 1553 1071 926 901 743 262 197 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 2251 3011 1132 Size: 1119 760 3774 Fragments arranged by size: 3774 1119 760 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 2796 3092 513 Size: 552 1731 296 3074 Fragments arranged by size: 3074 1731 552 296 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2194 2285 2406 2455 2591 3680 3697 Size: 1255 614 91 121 49 136 1089 17 Cuts at: 3697 3850 4035 4480 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1089 614 445 185 153 136 121 91 49 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2814 3899 5611 Size: 274 80 33 51 1443 837 1085 1712 Cuts at: 5611 96 Size: 138 Fragments arranged by size: 1712 1443 1085 837 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5613 5645 5613 Size: 32 5621 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5653 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2287 2287 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI