Restriction map for
pFN21AE0856
(Linear) (Six-base) MAPSORT of: KIEE0856.seq Check: 9089 from: 1 to: 948
KIEE0856.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 948
Size: 916 32
AleI CACnn'nnGTG
Cuts at: 0 283 745 948
Size: 283 462 203
Fragments arranged by size:
462 283 203
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 149 181 948
Size: 149 32 767
Fragments arranged by size:
767 149 32
AlwNI CAG_nnn'CTG
Cuts at: 0 72 948
Size: 72 876
ApoI r'AATT_y
Cuts at: 0 31 948
Size: 31 917
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 163 196 713 746 948
Size: 163 33 517 33 202
Fragments arranged by size:
517 202 163 33 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 654 837 948
Size: 654 183 111
Fragments arranged by size:
654 183 111
BbvCI CC'TCA_GC
Cuts at: 0 698 859 948
Size: 698 161 89
Fragments arranged by size:
698 161 89
BciVI GTATCCnnnnn_n'
Cuts at: 0 741 948
Size: 741 207
BclI T'GATC_A
Cuts at: 0 363 948
Size: 363 585
BglI GCCn_nnn'nGGC
Cuts at: 0 826 948
Size: 826 122
Bme1580I G_kGCm'C
Cuts at: 0 198 429 948
Size: 198 231 519
Fragments arranged by size:
519 231 198
Bpu10I CC'TnA_GC
Cuts at: 0 698 859 948
Size: 698 161 89
Fragments arranged by size:
698 161 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 948
Size: 702 246
BsaAI yAC'GTr
Cuts at: 0 372 948
Size: 372 576
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 149 179 948
Size: 149 30 769
Fragments arranged by size:
769 149 30
BseYI C'CCAG_C
Cuts at: 0 62 948
Size: 62 886
BsmI GAATG_Cn'
Cuts at: 0 906 948
Size: 906 42
Bsp1286I G_dGCh'C
Cuts at: 0 198 429 948
Size: 198 231 519
Fragments arranged by size:
519 231 198
BsrDI GCAATG_nn'
Cuts at: 0 115 948
Size: 115 833
BsrFI r'CCGG_y
Cuts at: 0 704 948
Size: 704 244
BsrGI T'GTAC_A
Cuts at: 0 809 948
Size: 809 139
BstAPI GCAn_nnn'nTGC
Cuts at: 0 72 948
Size: 72 876
BtgI C'CryG_G
Cuts at: 0 417 948
Size: 417 531
BtsI GCAGTG_nn'
Cuts at: 0 388 948
Size: 388 560
DraIII CAC_nnn'GTG
Cuts at: 0 372 948
Size: 372 576
EaeI y'GGCC_r
Cuts at: 0 140 827 948
Size: 140 687 121
Fragments arranged by size:
687 140 121
EarI CTCTTCn'nnn_
Cuts at: 0 190 265 948
Size: 190 75 683
Fragments arranged by size:
683 190 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 948
Size: 916 32
EcoO109I rG'GnC_Cy
Cuts at: 0 795 948
Size: 795 153
MscI TGG'CCA
Cuts at: 0 142 829 948
Size: 142 687 119
Fragments arranged by size:
687 142 119
MslI CAynn'nnrTG
Cuts at: 0 175 283 301 352 406 469 598 745
Size: 175 108 18 51 54 63 129 147
Cuts at: 745 948
Size: 203
Fragments arranged by size:
203 175 147 129 108 63 54 51
18
NaeI GCC'GGC
Cuts at: 0 706 948
Size: 706 242
NgoMIV G'CCGG_C
Cuts at: 0 704 948
Size: 704 244
NspI r_CATG'y
Cuts at: 0 246 405 948
Size: 246 159 543
Fragments arranged by size:
543 246 159
PflMI CCAn_nnn'nTGG
Cuts at: 0 149 435 948
Size: 149 286 513
Fragments arranged by size:
513 286 149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 149 181 202 234 948
Size: 149 32 21 32 714
Fragments arranged by size:
714 149 32 32 21
SapI GCTCTTCn'nnn_
Cuts at: 0 265 948
Size: 265 683
SfcI C'TryA_G
Cuts at: 0 15 759 948
Size: 15 744 189
Fragments arranged by size:
744 189 15
SmlI C'TyrA_G
Cuts at: 0 681 948
Size: 681 267
SphI G_CATG'C
Cuts at: 0 246 948
Size: 246 702
StyI C'CwwG_G
Cuts at: 0 791 948
Size: 791 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 189 280 401 450 586 948
Size: 189 91 121 49 136 362
Fragments arranged by size:
362 189 136 121 91 49
TatI w'GTAC_w
Cuts at: 0 809 948
Size: 809 139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 282 948
Size: 282 666
Enzymes that do cut:
AcuI AleI AloI AlwNI ApoI BaeI BbsI BbvCI
BciVI BclI BglI Bme1580I Bpu10I BpuEI BsaAI BsaXI
BseYI BsmI Bsp1286I BsrDI BsrFI BsrGI BstAPI BtgI
BtsI DraIII EaeI EarI Eco57MI EcoO109I MscI MslI
NaeI NgoMIV NspI PflMI PpiI SapI SfcI SmlI
SphI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BcgI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BpmI BsaI
BsaBI BsaHI BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI