(Circular) (Six-base) MAPSORT of: pFN21AE0855.seq Check: 4697 from: 1 to: 5638 pFN21AE0855 5638 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3448 278 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2981 1937 Size: 1044 4594 Acc65I G'GTAC_C Cuts at: 2959 2959 Size: 5638 AclI AA'CG_TT Cuts at: 1563 3765 4138 1563 Size: 2202 373 3063 Fragments arranged by size: 3063 2202 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3701 5085 5353 1632 Size: 2069 1384 268 1917 Fragments arranged by size: 2069 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5441 AflIII A'CryG_T Cuts at: 1203 2208 4537 5514 1203 Size: 1005 2329 977 1327 Fragments arranged by size: 2329 1327 1005 977 AgeI A'CCGG_T Cuts at: 4469 4469 Size: 5638 AhdI GACnn_n'nnGTC Cuts at: 4367 4367 Size: 5638 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5638 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5638 AlwNI CAG_nnn'CTG Cuts at: 3381 4953 3381 Size: 1572 4066 ApaLI G'TGCA_C Cuts at: 3695 4851 3695 Size: 1156 4482 ApoI r'AATT_y Cuts at: 1493 2947 3173 3325 5434 1493 Size: 1454 226 152 2109 1697 Fragments arranged by size: 2109 1697 1454 226 152 AseI AT'TA_AT Cuts at: 160 1032 4192 160 Size: 872 3160 1606 Fragments arranged by size: 3160 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 AvaI C'yCGr_G Cuts at: 1870 1943 2623 2963 1870 Size: 73 680 340 4545 Fragments arranged by size: 4545 680 340 73 BamHI G'GATC_C Cuts at: 2682 2968 3335 2682 Size: 286 367 4985 Fragments arranged by size: 4985 367 286 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2230 2959 4414 618 Size: 325 217 627 443 729 1455 1842 Fragments arranged by size: 1842 1455 729 627 443 325 217 BanII G_rGCy'C Cuts at: 1391 2957 1391 Size: 1566 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2080 928 Size: 971 181 4486 Fragments arranged by size: 4486 971 181 BbvCI CC'TCA_GC Cuts at: 2578 2578 Size: 5638 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3829 3863 1903 Size: 34 1892 34 3678 Fragments arranged by size: 3678 1892 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3530 4740 3530 Size: 1210 4428 BclI T'GATC_A Cuts at: 1548 2142 1548 Size: 594 5044 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2432 4249 136 Size: 107 122 71 631 605 760 1817 1525 Fragments arranged by size: 1817 1525 760 631 605 122 107 71 BglII A'GATC_T Cuts at: 5633 5633 Size: 5638 BlpI GC'TnA_GC Cuts at: 2298 3055 2298 Size: 757 4881 Bme1580I G_kGCm'C Cuts at: 1790 2235 2428 3699 4855 1790 Size: 445 193 1271 1156 2573 Fragments arranged by size: 2573 1271 1156 445 193 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5638 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2421 3369 4327 Size: 416 53 83 399 24 1004 948 958 Cuts at: 4327 442 Size: 1753 Fragments arranged by size: 1753 1004 958 948 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2776 4298 1239 Size: 1537 1522 2579 Fragments arranged by size: 2579 1537 1522 Bpu10I CC'TnA_GC Cuts at: 2390 2578 2928 2390 Size: 188 350 5100 Fragments arranged by size: 5100 350 188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3763 4628 4926 3763 Size: 865 298 4475 Fragments arranged by size: 4475 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4301 882 Size: 617 2802 2219 Fragments arranged by size: 2802 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2104 3429 493 Size: 711 900 1325 2702 Fragments arranged by size: 2702 1325 900 711 BsaBI GATnn'nnATC Cuts at: 2594 5632 2594 Size: 3038 2600 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 2799 3445 3827 Size: 53 83 186 936 537 729 646 382 Cuts at: 3827 4462 275 Size: 635 1451 Fragments arranged by size: 1451 936 729 646 635 537 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4071 4469 4743 4890 5301 4071 Size: 398 274 147 411 4408 Fragments arranged by size: 4408 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2824 2854 1178 Size: 30 165 30 1421 30 3962 Fragments arranged by size: 3962 1421 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2832 1186 Size: 931 715 3992 Fragments arranged by size: 3992 931 715 BseYI C'CCAG_C Cuts at: 2116 4841 5337 2116 Size: 2725 496 2417 Fragments arranged by size: 2725 2417 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2447 2589 1163 Size: 573 711 142 4212 Fragments arranged by size: 4212 711 573 142 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3088 3849 4877 1540 Size: 275 186 1087 761 1028 2301 Fragments arranged by size: 2301 1087 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2109 2330 3699 3784 4855 2109 Size: 221 1369 85 1071 2892 Fragments arranged by size: 2892 1369 1071 221 85 BsmI GAATG_Cn' Cuts at: 3149 3242 3149 Size: 93 5545 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2235 2330 2428 2957 3699 3784 Size: 399 319 126 95 98 529 742 85 Cuts at: 3784 4855 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 529 399 319 126 98 95 85 BspEI T'CCGG_A Cuts at: 5301 5301 Size: 5638 BspHI T'CATG_A Cuts at: 3527 5257 3527 Size: 1730 3908 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2993 844 Size: 2149 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2389 3525 1394 Size: 429 566 1136 3507 Fragments arranged by size: 3507 1136 566 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4133 4307 65 Size: 1183 2885 174 1396 Fragments arranged by size: 2885 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2438 4282 4469 1700 Size: 110 628 1844 187 2869 Fragments arranged by size: 2869 1844 628 187 110 BsrGI T'GTAC_A Cuts at: 96 2862 96 Size: 2766 2872 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5638 BssSI C'ACGA_G Cuts at: 2419 3698 4710 2419 Size: 1279 1012 3347 Fragments arranged by size: 3347 1279 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5638 BstBI TT'CG_AA Cuts at: 4442 4442 Size: 5638 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5638 BstXI CCAn_nnnn'nTGG Cuts at: 2590 2590 Size: 5638 BstYI r'GATC_y Cuts at: 1972 2520 2682 2968 3335 3720 3737 5189 5275 Size: 548 162 286 367 385 17 1452 86 Cuts at: 5275 5287 5633 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 548 385 367 346 286 162 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2780 2780 Size: 5638 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5086 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4899 Fragments arranged by size: 4899 683 56 BtsI GCAGTG_nn' Cuts at: 3154 3948 3968 3154 Size: 794 20 4824 Fragments arranged by size: 4824 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5638 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5603 DraI TTT'AAA Cuts at: 2942 2942 Size: 5638 DraIII CAC_nnn'GTG Cuts at: 2334 2334 Size: 5638 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2254 4645 809 Size: 507 938 2391 1802 Fragments arranged by size: 2391 1802 938 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2373 2433 2817 Size: 54 1425 50 275 18 543 60 384 Cuts at: 2817 3085 3974 5603 8 Size: 268 889 1629 43 Fragments arranged by size: 1629 1425 889 543 384 275 268 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3085 1537 Size: 275 1273 4090 Fragments arranged by size: 4090 1273 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3568 1353 Size: 2215 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4225 4599 4745 387 Size: 1064 138 2636 374 146 1280 Fragments arranged by size: 2636 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2776 3701 4298 5085 5353 1239 Size: 393 1144 925 597 787 268 1524 Fragments arranged by size: 1524 1144 925 787 597 393 268 EcoRI G'AATT_C Cuts at: 2947 3325 2947 Size: 378 5260 FspI TGC'GCA Cuts at: 4144 4144 Size: 5638 HaeII r_GCGC'y Cuts at: 1678 2419 4785 1678 Size: 741 2366 2531 Fragments arranged by size: 2531 2366 741 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4279 4311 4353 4385 1373 Size: 32 135 32 2707 32 42 32 2626 Fragments arranged by size: 2707 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2982 3228 669 Size: 988 281 1044 246 3079 Fragments arranged by size: 3079 1044 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5638 HpaI GTT'AAC Cuts at: 3228 3228 Size: 5638 KpnI G_GTAC'C Cuts at: 2963 2963 Size: 5638 MfeI C'AATT_G Cuts at: 3237 3237 Size: 5638 MluI A'CGCG_T Cuts at: 5514 5514 Size: 5638 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2034 4752 4936 1305 Size: 729 2718 184 2007 Fragments arranged by size: 2718 2007 729 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2375 2435 10 Size: 54 1425 343 543 60 3213 Fragments arranged by size: 3213 1425 543 343 60 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 2207 2351 2402 2600 3596 3955 Size: 648 1005 36 144 51 198 996 359 Cuts at: 3955 4114 518 Size: 159 2042 Fragments arranged by size: 2042 1005 996 648 359 198 159 144 51 36 MspA1I CmG'CkG Cuts at: 1606 2289 3054 3731 4879 5124 1606 Size: 683 765 677 1148 245 2120 Fragments arranged by size: 2120 1148 765 683 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5086 NdeI CA'TA_TG Cuts at: 387 387 Size: 5638 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5638 NotI GC'GGCC_GC Cuts at: 3085 3085 Size: 5638 NspI r_CATG'y Cuts at: 1132 1229 2212 2996 4541 1132 Size: 97 983 784 1545 2229 Fragments arranged by size: 2229 1545 983 784 97 PasI CC'CwG_GG Cuts at: 1348 2060 1348 Size: 712 4926 PciI A'CATG_T Cuts at: 2208 4537 2208 Size: 2329 3309 PflMI CCAn_nnn'nTGG Cuts at: 1108 2081 2320 1108 Size: 973 239 4426 Fragments arranged by size: 4426 973 239 PmeI GTTT'AAAC Cuts at: 2942 2942 Size: 5638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3707 3739 5220 5252 3707 Size: 32 1481 32 4093 Fragments arranged by size: 4093 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5638 PsiI TTA'TAA Cuts at: 3208 3208 Size: 5638 PstI C_TGCA'G Cuts at: 830 2990 830 Size: 2160 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5638 PvuII CAG'CTG Cuts at: 2289 2289 Size: 5638 SalI G'TCGA_C Cuts at: 1936 2980 1936 Size: 1044 4594 SbfI CC_TGCA'GG Cuts at: 2990 2990 Size: 5638 ScaI AGT'ACT Cuts at: 3886 5598 3886 Size: 1712 3926 SfcI C'TryA_G Cuts at: 826 1045 2986 4121 4802 4993 826 Size: 219 1941 1135 681 191 1471 Fragments arranged by size: 1941 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5638 SmaI CCC'GGG Cuts at: 1872 2965 1872 Size: 1093 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3742 4643 4905 820 Size: 197 926 1799 901 262 1553 Fragments arranged by size: 1799 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3429 493 Size: 2936 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5638 SphI G_CATG'C Cuts at: 1132 2996 1132 Size: 1864 3774 SspI AAT'ATT Cuts at: 5 52 2608 3562 5 Size: 47 2556 954 2081 Fragments arranged by size: 2556 2081 954 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5638 StyI C'CwwG_G Cuts at: 513 1065 2250 3077 513 Size: 552 1185 827 3074 Fragments arranged by size: 3074 1185 827 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3665 3682 3835 4020 4465 325 Size: 1255 2085 17 153 185 445 1498 Fragments arranged by size: 2085 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2175 2862 3884 Size: 274 80 33 51 1443 198 687 1022 Cuts at: 3884 5596 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1022 687 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2032 2064 2535 2567 2824 2856 5598 5630 2032 Size: 32 471 32 257 32 2742 32 2040 Fragments arranged by size: 2742 2040 471 257 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5638 XbaI T'CTAG_A Cuts at: 2974 2974 Size: 5638 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2557 2557 Size: 5638 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5638 XmaI C'CCGG_G Cuts at: 1870 2963 1870 Size: 1093 4545 XmnI GAAnn'nnTTC Cuts at: 3767 3767 Size: 5638 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3446 276 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI