Restriction map for
pFN21AE0854
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DpnII DraI DraII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HindIII HpaI KpnI KspI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SapI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 583 GTCGAC GT^[AC][GT]AC
AcsI 743 GAATTC [GA]^AATT[CT]
AcyI 761 GGCGCC G[GA]^CG[CT]C
AfaI 389 GTAC GT^AC
AflIII 781 ACGTGT A^C[GA][CT]GT
AlwNI 477 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 743 GAATTC [GA]^AATT[CT]
BbeI 761 GGCGCC GGCGC^C
BbrPI 780 CACGTG CAC^GTG
BglI 631 GCCGATACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 780 CACGTG [CT]AC^GT[GA]
BsaHI 761 GGCGCC G[GA]^CG[CT]C
BseRI 169 CTCCTC GAGGAG(10/8)
BsmAI 90 GTCTC GTCTC(1/5)
BspHI 467 TCATGA T^CATGA
BsrDI 767 GCAATG GCAATG(2/0)
BstEII 654 GGTCACC G^GT[ACGT]ACC
BstPI 654 GGTCACC G^GT[ACGT]ACC
BstXI 299 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 927 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 81 GACTTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 638 CGGCCA [CT]^GGCC[GA]
EarI 142 CTCTTC CTCTTC(1/4)
EcoO65I 654 GGTCACC G^GT[ACGT]ACC
EcoRI 743 GAATTC G^AATTC
HgaI 618 GCGTC GACGC(5/10)
Hin1I 761 GGCGCC G[GA]^CG[CT]C
HincII 583 GTCGAC GT[CT]^[GA]AC
HindII 583 GTCGAC GT[CT]^[GA]AC
HinfI 479 GAGTC G^A[ACGT]TC
KasI 761 GGCGCC G^GCGCC
Ksp632I 142 CTCTTC CTCTTC(1/4)
MaeII 781 ACGT A^CGT
MseI 911 TTAA T^TAA
MspA1I 127 CAGCTG C[AC]G^C[GT]G
NarI 761 GGCGCC GG^CGCC
PflMI 375 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 479 GAGTC GAGTC(4/5)
PmaCI 780 CACGTG CAC^GTG
PmlI 780 CACGTG CAC^GTG
PvuII 127 CAGCTG CAG^CTG
RcaI 467 TCATGA T^CATGA
RsaI 389 GTAC GT^AC
SalI 583 GTCGAC G^TCGAC
SfoI 761 GGCGCC GGC^GCC
TaqI 584 TCGA T^CGA
Tru9I 911 TTAA T^TAA
Tsp509I 744 AATT ^AATT
TthHB8I 584 TCGA T^CGA
Van91I 375 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 641 CCATCAACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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AciI 765 CCGC CCGC(-3/-1)
843 GCGG
BanI 502 GGCACC G^G[CT][GA]CC
761 GGCGCC
BanII 190 GGGCTC G[GA]GC[CT]^C
859 GAGCCC
BfaI 622 CTAG C^TAG
967 CTAG
BspMI 688 ACCTGC ACCTGC(4/8)
839 ACCTGC
HaeII 135 AGCGCC [GA]GCGC^[CT]
761 GGCGCC
HapII 222 CCGG C^CGG
555 CCGG
HpaII 222 CCGG C^CGG
555 CCGG
MaeI 622 CTAG C^TAG
967 CTAG
MboII 143 TCTTC GAAGA(8/7)
593 TCTTC
MslI 99 CATTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
408 CACACTCATG
MspI 222 CCGG C^CGG
555 CCGG
MwoI 129 GCTGGGAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
631 GCCGATACGGC
SfaNI 303 GATGC GCATC(5/9)
18 GCATC
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI BbvI BmyI BsaJI BsgI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HphI ItaI MaeIII
MnlI MvaI NlaIII NlaIV Sau96I
ScrFI TseI Tsp45I TspRI