(Circular) (Six-base) MAPSORT of: pFN21AE0854.seq Check: 6558 from: 1 to: 5701 pFN21AE0854 5701 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2702 2702 Size: 5701 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3511 278 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2589 3044 1937 Size: 652 455 4594 Fragments arranged by size: 4594 652 455 Acc65I G'GTAC_C Cuts at: 3022 3022 Size: 5701 AclI AA'CG_TT Cuts at: 1563 3828 4201 1563 Size: 2265 373 3063 Fragments arranged by size: 3063 2265 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3764 5148 5416 1632 Size: 2132 1384 268 1917 Fragments arranged by size: 2132 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5504 AflIII A'CryG_T Cuts at: 1203 2786 4600 5577 1203 Size: 1583 1814 977 1327 Fragments arranged by size: 1814 1583 1327 977 AgeI A'CCGG_T Cuts at: 4532 4532 Size: 5701 AhdI GACnn_n'nnGTC Cuts at: 4430 4430 Size: 5701 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5701 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2084 1184 Size: 900 4801 AlwNI CAG_nnn'CTG Cuts at: 2487 3444 5016 2487 Size: 957 1572 3172 Fragments arranged by size: 3172 1572 957 ApaLI G'TGCA_C Cuts at: 3758 4914 3758 Size: 1156 4545 ApoI r'AATT_y Cuts at: 1493 2748 3010 3236 3388 5497 1493 Size: 1255 262 226 152 2109 1697 Fragments arranged by size: 2109 1697 1255 262 226 152 AseI AT'TA_AT Cuts at: 160 1032 4255 160 Size: 872 3223 1606 Fragments arranged by size: 3223 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 AvaI C'yCGr_G Cuts at: 1870 1943 3026 1870 Size: 73 1083 4545 Fragments arranged by size: 4545 1083 73 BamHI G'GATC_C Cuts at: 3031 3398 3031 Size: 367 5334 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2507 2766 3022 4477 618 Size: 325 217 627 720 259 256 1455 1842 Fragments arranged by size: 1842 1455 720 627 325 259 256 217 BanII G_rGCy'C Cuts at: 1391 2199 2868 3020 1391 Size: 808 669 152 4072 Fragments arranged by size: 4072 808 669 152 BbeI G_GCGC'C Cuts at: 2770 2770 Size: 5701 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4730 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3892 3926 1903 Size: 34 1955 34 3678 Fragments arranged by size: 3678 1955 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3593 4803 3593 Size: 1210 4491 BclI T'GATC_A Cuts at: 1548 1548 Size: 5701 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2642 4312 136 Size: 107 122 71 631 605 970 1670 1525 Fragments arranged by size: 1670 1525 970 631 605 122 107 71 BglII A'GATC_T Cuts at: 5696 5696 Size: 5701 BlpI GC'TnA_GC Cuts at: 3118 3118 Size: 5701 Bme1580I G_kGCm'C Cuts at: 1790 2829 3762 4918 1790 Size: 1039 933 1156 2573 Fragments arranged by size: 2573 1156 1039 933 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5701 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3432 4390 442 Size: 416 53 83 399 24 2015 958 1753 Fragments arranged by size: 2015 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5701 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2162 2162 Size: 5701 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4361 1239 Size: 3122 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3826 4691 4989 3826 Size: 865 298 4538 Fragments arranged by size: 4538 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4364 882 Size: 617 2865 2219 Fragments arranged by size: 2865 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2787 3492 493 Size: 711 1583 705 2702 Fragments arranged by size: 2702 1583 711 705 BsaBI GATnn'nnATC Cuts at: 5695 5695 Size: 5701 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2767 3508 3890 4525 Size: 53 83 186 936 1234 741 382 635 Cuts at: 4525 275 Size: 1451 Fragments arranged by size: 1451 1234 936 741 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4134 4532 4806 4953 5364 4134 Size: 398 274 147 411 4471 Fragments arranged by size: 4471 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5476 Fragments arranged by size: 5476 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 1186 Size: 979 4722 BseYI C'CCAG_C Cuts at: 2134 2515 4904 5400 2134 Size: 381 2389 496 2435 Fragments arranged by size: 2435 2389 496 381 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2102 2815 3011 1163 Size: 573 366 713 196 3853 Fragments arranged by size: 3853 713 573 366 196 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3151 3912 4940 1540 Size: 275 186 1150 761 1028 2301 Fragments arranged by size: 2301 1150 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3762 3847 4918 3762 Size: 85 1071 4545 Fragments arranged by size: 4545 1071 85 BsmI GAATG_Cn' Cuts at: 3212 3305 3212 Size: 93 5608 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2199 2829 2868 3020 3762 3847 4918 Size: 399 409 630 39 152 742 85 1071 Cuts at: 4918 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 630 409 399 152 85 39 BspEI T'CCGG_A Cuts at: 5364 5364 Size: 5701 BspHI T'CATG_A Cuts at: 2472 3590 5320 2472 Size: 1118 1730 2853 Fragments arranged by size: 2853 1730 1118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2702 2853 3056 844 Size: 1858 151 203 3489 Fragments arranged by size: 3489 1858 203 151 BsrBI CCG'CTC Cuts at: 1394 1823 3588 1394 Size: 429 1765 3507 Fragments arranged by size: 3507 1765 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2779 4196 4370 65 Size: 1183 1531 1417 174 1396 Fragments arranged by size: 1531 1417 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4345 4532 1700 Size: 110 2535 187 2869 Fragments arranged by size: 2869 2535 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5701 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5701 BssSI C'ACGA_G Cuts at: 3761 4773 3761 Size: 1012 4689 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5701 BstBI TT'CG_AA Cuts at: 4505 4505 Size: 5701 BstEII G'GTnAC_C Cuts at: 1184 2659 1184 Size: 1475 4226 BstXI CCAn_nnnn'nTGG Cuts at: 2311 2311 Size: 5701 BstYI r'GATC_y Cuts at: 1972 3031 3398 3783 3800 5252 5338 5350 5696 Size: 1059 367 385 17 1452 86 12 346 Cuts at: 5696 1972 Size: 1977 Fragments arranged by size: 1977 1452 1059 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5149 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4962 Fragments arranged by size: 4962 683 56 BtsI GCAGTG_nn' Cuts at: 3217 4011 4031 3217 Size: 794 20 4887 Fragments arranged by size: 4887 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5701 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5666 DraI TTT'AAA Cuts at: 3005 3005 Size: 5701 DraIII CAC_nnn'GTG Cuts at: 2937 2937 Size: 5701 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2092 4708 809 Size: 507 776 2616 1802 Fragments arranged by size: 2616 1802 776 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2643 3148 4037 Size: 54 1425 50 275 18 813 505 889 Cuts at: 4037 5666 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 813 505 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3148 1537 Size: 275 1336 4090 Fragments arranged by size: 4090 1336 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2153 3631 1353 Size: 800 1478 3423 Fragments arranged by size: 3423 1478 800 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4288 4662 4808 387 Size: 1064 138 2699 374 146 1280 Fragments arranged by size: 2699 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3764 4361 5148 5416 1239 Size: 393 2132 597 787 268 1524 Fragments arranged by size: 2132 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2748 3010 3388 2748 Size: 262 378 5061 Fragments arranged by size: 5061 378 262 FspI TGC'GCA Cuts at: 4207 4207 Size: 5701 HaeII r_GCGC'y Cuts at: 1678 2144 2770 4848 1678 Size: 466 626 2078 2531 Fragments arranged by size: 2531 2078 626 466 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2511 2543 4342 4374 4416 Size: 32 135 32 939 32 1799 32 42 Cuts at: 4416 4448 1373 Size: 32 2626 Fragments arranged by size: 2626 1799 939 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2590 3045 3291 669 Size: 988 281 652 455 246 3079 Fragments arranged by size: 3079 988 652 455 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5701 HpaI GTT'AAC Cuts at: 3291 3291 Size: 5701 KasI G'GCGC_C Cuts at: 2766 2766 Size: 5701 KpnI G_GTAC'C Cuts at: 3026 3026 Size: 5701 MfeI C'AATT_G Cuts at: 3300 3300 Size: 5701 MluI A'CGCG_T Cuts at: 5577 5577 Size: 5701 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2389 4815 4999 1305 Size: 1084 2426 184 2007 Fragments arranged by size: 2426 2007 1084 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3879 Fragments arranged by size: 3879 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2108 2417 3659 4018 4177 518 Size: 648 942 309 1242 359 159 2042 Fragments arranged by size: 2042 1242 942 648 359 309 159 MspA1I CmG'CkG Cuts at: 1606 2134 3117 3794 4942 5187 1606 Size: 528 983 677 1148 245 2120 Fragments arranged by size: 2120 1148 983 677 528 245 NarI GG'CG_CC Cuts at: 2767 2767 Size: 5701 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5149 NdeI CA'TA_TG Cuts at: 387 387 Size: 5701 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5701 NotI GC'GGCC_GC Cuts at: 3148 3148 Size: 5701 NspI r_CATG'y Cuts at: 1132 1229 3059 4604 1132 Size: 97 1830 1545 2229 Fragments arranged by size: 2229 1830 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5701 PciI A'CATG_T Cuts at: 4600 4600 Size: 5701 PflMI CCAn_nnn'nTGG Cuts at: 1108 2386 1108 Size: 1278 4423 PmeI GTTT'AAAC Cuts at: 3005 3005 Size: 5701 PmlI CAC'GTG Cuts at: 2787 2787 Size: 5701 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3770 3802 5283 5315 3770 Size: 32 1481 32 4156 Fragments arranged by size: 4156 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5701 PsiI TTA'TAA Cuts at: 3271 3271 Size: 5701 PstI C_TGCA'G Cuts at: 830 3053 830 Size: 2223 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5701 PvuII CAG'CTG Cuts at: 2134 2134 Size: 5701 SalI G'TCGA_C Cuts at: 1936 2588 3043 1936 Size: 652 455 4594 Fragments arranged by size: 4594 652 455 SbfI CC_TGCA'GG Cuts at: 3053 3053 Size: 5701 ScaI AGT'ACT Cuts at: 3949 5661 3949 Size: 1712 3989 SfcI C'TryA_G Cuts at: 826 1045 3049 4184 4865 5056 826 Size: 219 2004 1135 681 191 1471 Fragments arranged by size: 2004 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2768 2768 Size: 5701 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5701 SmaI CCC'GGG Cuts at: 1872 3028 1872 Size: 1156 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3805 4706 4968 820 Size: 197 926 1862 901 262 1553 Fragments arranged by size: 1862 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3492 493 Size: 2999 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5701 SphI G_CATG'C Cuts at: 1132 3059 1132 Size: 1927 3774 SspI AAT'ATT Cuts at: 5 52 3625 5 Size: 47 3573 2081 Fragments arranged by size: 3573 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5701 StyI C'CwwG_G Cuts at: 513 1065 3140 513 Size: 552 2075 3074 Fragments arranged by size: 3074 2075 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2139 3728 3745 3898 4083 4528 325 Size: 1255 559 1589 17 153 185 445 1498 Fragments arranged by size: 1589 1498 1255 559 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2393 3947 5659 Size: 274 80 33 51 1443 416 1554 1712 Cuts at: 5659 96 Size: 138 Fragments arranged by size: 1712 1554 1443 416 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5661 5693 5661 Size: 32 5669 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5701 XbaI T'CTAG_A Cuts at: 3037 3037 Size: 5701 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2653 2653 Size: 5701 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5701 XmaI C'CCGG_G Cuts at: 1870 3026 1870 Size: 1156 4545 XmnI GAAnn'nnTTC Cuts at: 3830 3830 Size: 5701 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3509 276 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI