Restriction map for
pFN21AE0854
(Linear) (Six-base) MAPSORT of: KIEE0854.seq Check: 8890 from: 1 to: 996
KIEE0854.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 697 996
Size: 697 299
AccI GT'mk_AC
Cuts at: 0 584 996
Size: 584 412
AflIII A'CryG_T
Cuts at: 0 781 996
Size: 781 215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 79 996
Size: 79 917
AlwNI CAG_nnn'CTG
Cuts at: 0 482 996
Size: 482 514
ApoI r'AATT_y
Cuts at: 0 743 996
Size: 743 253
BanI G'GyrC_C
Cuts at: 0 502 761 996
Size: 502 259 235
Fragments arranged by size:
502 259 235
BanII G_rGCy'C
Cuts at: 0 194 863 996
Size: 194 669 133
Fragments arranged by size:
669 194 133
BbeI G_GCGC'C
Cuts at: 0 765 996
Size: 765 231
BglI GCCn_nnn'nGGC
Cuts at: 0 637 996
Size: 637 359
Bme1580I G_kGCm'C
Cuts at: 0 824 996
Size: 824 172
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 157 996
Size: 157 839
BsaAI yAC'GTr
Cuts at: 0 782 996
Size: 782 214
BsaHI Gr'CG_yC
Cuts at: 0 762 996
Size: 762 234
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 996
Size: 160 836
BseYI C'CCAG_C
Cuts at: 0 129 510 996
Size: 129 381 486
Fragments arranged by size:
486 381 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 810 996
Size: 97 713 186
Fragments arranged by size:
713 186 97
Bsp1286I G_dGCh'C
Cuts at: 0 194 824 863 996
Size: 194 630 39 133
Fragments arranged by size:
630 194 133 39
BspHI T'CATG_A
Cuts at: 0 467 996
Size: 467 529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 697 848 996
Size: 697 151 148
Fragments arranged by size:
697 151 148
BsrDI GCAATG_nn'
Cuts at: 0 774 996
Size: 774 222
BstEII G'GTnAC_C
Cuts at: 0 654 996
Size: 654 342
BstXI CCAn_nnnn'nTGG
Cuts at: 0 306 996
Size: 306 690
DraIII CAC_nnn'GTG
Cuts at: 0 932 996
Size: 932 64
DrdI GACnn_nn'nnGTC
Cuts at: 0 87 996
Size: 87 909
EaeI y'GGCC_r
Cuts at: 0 638 996
Size: 638 358
EarI CTCTTCn'nnn_
Cuts at: 0 148 996
Size: 148 848
EcoRI G'AATT_C
Cuts at: 0 743 996
Size: 743 253
HaeII r_GCGC'y
Cuts at: 0 139 765 996
Size: 139 626 231
Fragments arranged by size:
626 231 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 506 538 996
Size: 506 32 458
Fragments arranged by size:
506 458 32
HincII GTy'rAC
Cuts at: 0 585 996
Size: 585 411
KasI G'GCGC_C
Cuts at: 0 761 996
Size: 761 235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 996
Size: 384 612
MslI CAynn'nnrTG
Cuts at: 0 103 412 996
Size: 103 309 584
Fragments arranged by size:
584 309 103
MspA1I CmG'CkG
Cuts at: 0 129 996
Size: 129 867
NarI GG'CG_CC
Cuts at: 0 762 996
Size: 762 234
PflMI CCAn_nnn'nTGG
Cuts at: 0 381 996
Size: 381 615
PmlI CAC'GTG
Cuts at: 0 782 996
Size: 782 214
PvuII CAG'CTG
Cuts at: 0 129 996
Size: 129 867
SalI G'TCGA_C
Cuts at: 0 583 996
Size: 583 413
SfoI GGC'GCC
Cuts at: 0 763 996
Size: 763 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 134 996
Size: 134 862
TatI w'GTAC_w
Cuts at: 0 388 996
Size: 388 608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 648 996
Size: 648 348
Enzymes that do cut:
AarI AccI AflIII AlfI AlwNI ApoI BanI BanII
BbeI BglI Bme1580I BplI BsaAI BsaHI BseRI BseYI
BsgI Bsp1286I BspHI BspMI BsrDI BstEII BstXI DraIII
DrdI EaeI EarI EcoRI HaeII Hin4I HincII KasI
MmeI MslI MspA1I NarI PflMI PmlI PvuII SalI
SfoI TaqII TatI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI EagI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI