Restriction map for
pFN21AE0853
##################################
# NO CUT ENZYMES ( 148 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
AhdI Alw44I AlwI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I CelII ClaI CpoI DpnII
DraI DraII EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I FauI FbaI FseI
FspI HgaI HincII HindII HindIII
HinfI HpaI KspI MamI MboI
MfeI MflI MluI MluNI MroI
MscI MunI NcoI NdeI NdeII
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 619 GACGTC GACGT^C
Acc65I 380 GGTACC G^GTACC
AccI 763 GTCTAC GT^[AC][GT]AC
AccII 559 CGCG CG^CG
AflIII 224 ACATGT A^C[GA][CT]GT
AocI 792 CCTTAGG CC^T[ACGT]AGG
ApaI 523 GGGCCC GGGCC^C
Asp718I 380 GGTACC G^GTACC
BanII 523 GGGCCC G[GA]GC[CT]^C
BbeI 716 GGCGCC GGCGC^C
BbsI 100 GTCTTC GAAGAC(2/6)
BcnI 720 CCGGG CC^[GC]GG
BfaI 385 CTAG C^TAG
BpmI 452 CTGGAG CTGGAG(16/14)
BpuAI 100 GTCTTC GAAGAC(2/6)
BsgI 403 CTGCAC GTGCAG(16/14)
BsmFI 772 GTCCC GGGAC(10/14)
Bsp1407I 191 TGTACA T^GTACA
BspHI 419 TCATGA T^CATGA
BspLU11I 224 ACATGT A^CATGT
BsrFI 466 GCCGGC [GA]^CCGG[CT]
BsrGI 191 TGTACA T^GTACA
BstAPI 341 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 559 CGCG CG^CG
BstXI 527 CCAATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 792 CCTTAGG CC^T[ACGT]AGG
Cfr10I 466 GCCGGC [GA]^CCGG[CT]
DrdI 586 GACACAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 32 CCGTGG C^C[GA][CT]GG
EaeI 632 TGGCCG [CT]^GGCC[GA]
Eco81I 792 CCTTAGG CC^T[ACGT]AGG
EcoNI 371 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 475 ATGCAT ATGCA^T
HaeII 716 GGCGCC [GA]GCGC^[CT]
KasI 716 GGCGCC G^GCGCC
KpnI 380 GGTACC GGTAC^C
MaeI 385 CTAG C^TAG
MaeII 620 ACGT A^CGT
MseI 652 TTAA T^TAA
MslI 360 CACCTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 559 CGCG CG^CG
NaeI 466 GCCGGC GCC^GGC
NarI 716 GGCGCC GG^CGCC
NciI 720 CCGGG CC^[GC]GG
NgoMIV 466 GCCGGC G^CCGGC
NsiI 475 ATGCAT ATGCA^T
NspI 224 ACATGT [GA]CATG^[CT]
PvuII 127 CAGCTG CAG^CTG
RcaI 419 TCATGA T^CATGA
SfaNI 28 GATGC GCATC(5/9)
SfoI 716 GGCGCC GGC^GCC
SspBI 191 TGTACA T^GTACA
Tru9I 652 TTAA T^TAA
Tsp509I 654 AATT ^AATT
XcmI 806 CCAAAGACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 619 GACGTC G[GA]^CG[CT]C
716 GGCGCC
AfaI 192 GTAC GT^AC
381 GTAC
AlwNI 397 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
827 CAGCGGCTG
BanI 380 GGTACC G^G[CT][GA]CC
716 GGCGCC
BbvI 338 GCAGC GCAGC(8/12)
826 GCAGC
BsaHI 619 GACGTC G[GA]^CG[CT]C
716 GGCGCC
BseRI 88 CTCCTC GAGGAG(10/8)
234 CTCCTC
BsmAI 570 GAGAC GTCTC(1/5)
63 GTCTC
BspMI 137 ACCTGC ACCTGC(4/8)
925 GCAGGT
CfoI 560 GCGC GCG^C
717 GCGC
DraIII 153 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
342 CACCCAGTG
EarI 91 CTCTTC CTCTTC(1/4)
912 GAAGAG
HapII 467 CCGG C^CGG
720 CCGG
HhaI 560 GCGC GCG^C
717 GCGC
Hin1I 619 GACGTC G[GA]^CG[CT]C
716 GGCGCC
HinP1I 560 GCGC G^CGC
717 GCGC
HpaII 467 CCGG C^CGG
720 CCGG
Ksp632I 91 CTCTTC CTCTTC(1/4)
912 GAAGAG
MspI 467 CCGG C^CGG
720 CCGG
RsaI 192 GTAC GT^AC
381 GTAC
TseI 338 GCAGC G^C[AT]GC
826 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI AspHI BmyI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HphI ItaI MaeIII MboII MnlI
MspA1I MvaI MwoI NlaIII NlaIV
Sau96I ScrFI Tsp45I TspRI