Restriction map for
pFN21AE0853
(Linear) (Six-base) MAPSORT of: KIEE0853.seq Check: 5779 from: 1 to: 930
KIEE0853.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AatII G_ACGT'C
Cuts at: 0 623 930
Size: 623 307
AccI GT'mk_AC
Cuts at: 0 764 930
Size: 764 166
Acc65I G'GTAC_C
Cuts at: 0 380 930
Size: 380 550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 930
Size: 712 218
AflIII A'CryG_T
Cuts at: 0 224 930
Size: 224 706
AlwNI CAG_nnn'CTG
Cuts at: 0 402 832 930
Size: 402 430 98
Fragments arranged by size:
430 402 98
ApaI G_GGCC'C
Cuts at: 0 527 930
Size: 527 403
BanI G'GyrC_C
Cuts at: 0 380 716 930
Size: 380 336 214
Fragments arranged by size:
380 336 214
BanII G_rGCy'C
Cuts at: 0 527 930
Size: 527 403
BbeI G_GCGC'C
Cuts at: 0 720 930
Size: 720 210
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 930
Size: 93 837
BfrBI ATG'CAT
Cuts at: 0 477 930
Size: 477 453
Bme1580I G_kGCm'C
Cuts at: 0 527 930
Size: 527 403
BmrI ACTGGGnnnn_n'
Cuts at: 0 339 406 930
Size: 339 67 524
Fragments arranged by size:
524 339 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 930
Size: 473 457
BsaHI Gr'CG_yC
Cuts at: 0 620 717 930
Size: 620 97 213
Fragments arranged by size:
620 213 97
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 446 476 930
Size: 446 30 454
Fragments arranged by size:
454 446 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 225 930
Size: 79 146 705
Fragments arranged by size:
705 146 79
BseYI C'CCAG_C
Cuts at: 0 129 930
Size: 129 801
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 930
Size: 388 542
BsiHKAI G_wGCw'C
Cuts at: 0 183 212 419 930
Size: 183 29 207 511
Fragments arranged by size:
511 207 183 29
Bsp1286I G_dGCh'C
Cuts at: 0 183 212 419 527 930
Size: 183 29 207 108 403
Fragments arranged by size:
403 207 183 108 29
BspHI T'CATG_A
Cuts at: 0 419 930
Size: 419 511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 146 916 930
Size: 146 770 14
Fragments arranged by size:
770 146 14
BsrFI r'CCGG_y
Cuts at: 0 466 930
Size: 466 464
BsrGI T'GTAC_A
Cuts at: 0 191 930
Size: 191 739
BstAPI GCAn_nnn'nTGC
Cuts at: 0 347 930
Size: 347 583
BstXI CCAn_nnnn'nTGG
Cuts at: 0 534 930
Size: 534 396
Bsu36I CC'TnA_GG
Cuts at: 0 793 930
Size: 793 137
BtgI C'CryG_G
Cuts at: 0 32 930
Size: 32 898
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 5 930
Size: 5 925
DraIII CAC_nnn'GTG
Cuts at: 0 158 347 930
Size: 158 189 583
Fragments arranged by size:
583 189 158
DrdI GACnn_nn'nnGTC
Cuts at: 0 592 930
Size: 592 338
EaeI y'GGCC_r
Cuts at: 0 632 930
Size: 632 298
EarI CTCTTCn'nnn_
Cuts at: 0 97 907 930
Size: 97 810 23
Fragments arranged by size:
810 97 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 496 930
Size: 496 434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 712 930
Size: 473 239 218
Fragments arranged by size:
473 239 218
EcoNI CCTnn'n_nnAGG
Cuts at: 0 375 930
Size: 375 555
HaeII r_GCGC'y
Cuts at: 0 720 930
Size: 720 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 446 478 930
Size: 446 32 452
Fragments arranged by size:
452 446 32
KasI G'GCGC_C
Cuts at: 0 716 930
Size: 716 214
KpnI G_GTAC'C
Cuts at: 0 384 930
Size: 384 546
MslI CAynn'nnrTG
Cuts at: 0 364 930
Size: 364 566
MspA1I CmG'CkG
Cuts at: 0 129 706 829 930
Size: 129 577 123 101
Fragments arranged by size:
577 129 123 101
NaeI GCC'GGC
Cuts at: 0 468 930
Size: 468 462
NarI GG'CG_CC
Cuts at: 0 717 930
Size: 717 213
NgoMIV G'CCGG_C
Cuts at: 0 466 930
Size: 466 464
NsiI A_TGCA'T
Cuts at: 0 479 930
Size: 479 451
NspI r_CATG'y
Cuts at: 0 228 930
Size: 228 702
PciI A'CATG_T
Cuts at: 0 224 930
Size: 224 706
PfoI T'CCnGG_A
Cuts at: 0 218 930
Size: 218 712
PspOMI G'GGCC_C
Cuts at: 0 523 930
Size: 523 407
PvuII CAG'CTG
Cuts at: 0 129 930
Size: 129 801
SfoI GGC'GCC
Cuts at: 0 718 930
Size: 718 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 358 930
Size: 358 572
TatI w'GTAC_w
Cuts at: 0 191 930
Size: 191 739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 813 930
Size: 813 117
ZraI GAC'GTC
Cuts at: 0 621 930
Size: 621 309
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AlwNI ApaI BanI
BanII BbeI BbsI BfrBI Bme1580I BmrI BpmI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrFI BsrGI BstAPI BstXI Bsu36I BtgI BtgZI DraIII
DrdI EaeI EarI EciI Eco57MI EcoNI HaeII Hin4I
KasI KpnI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PciI PfoI PspOMI PvuII SfoI TaqII TatI
XcmI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BglI BglII
BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrBI
BsrDI BssHII BssSI BstBI BstEII BstYI BstZ17I BtsI
ClaI CspCI DraI EagI EcoICRI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI MfeI
MluI MmeI MscI NcoI NdeI NheI NotI NruI
PacI PasI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI