Restriction map for
pFN21AE0852
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BmyI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoRV EcoT22I FbaI
FseI FspI HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MamI MfeI
MflI MluI MroI MseI MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 25 GAATTC [GA]^AATT[CT]
AfaI 320 GTAC GT^AC
AlwI 465 GATCC GGATC(4/5)
AocI 861 CCTTAGG CC^T[ACGT]AGG
Aor51HI 718 AGCGCT AGC^GCT
ApoI 25 GAATTC [GA]^AATT[CT]
AvaII 434 GGACC G^G[AT]CC
AvrII 312 CCTAGG C^CTAGG
BalI 425 TGGCCA TGG^CCA
BlnI 312 CCTAGG C^CTAGG
BlpI 680 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 680 GCTCAGC GC^T[ACGT]AGC
BspHI 398 TCATGA T^CATGA
BspMI 712 ACCTGC ACCTGC(4/8)
BsrBI 678 CCGCTC CCGCTC(-3/-3)
BstEII 828 GGTAACC G^GT[ACGT]ACC
BstPI 828 GGTAACC G^GT[ACGT]ACC
Bsu36I 861 CCTTAGG CC^T[ACGT]AGG
CelII 680 GCTCAGC GC^T[ACGT]AGC
CfoI 719 GCGC GCG^C
DraIII 406 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 428 CCACGG C^C[GA][CT]GG
EaeI 425 TGGCCA [CT]^GGCC[GA]
EarI 213 CTCTTC CTCTTC(1/4)
Eco47III 718 AGCGCT AGC^GCT
Eco81I 861 CCTTAGG CC^T[ACGT]AGG
EcoO65I 828 GGTAACC G^GT[ACGT]ACC
EcoRI 25 GAATTC G^AATTC
EcoT14I 312 CCTAGG C^C[AT][AT]GG
FauI 699 GCGGG CCCGC(4/6)
HaeII 718 AGCGCT [GA]GCGC^[CT]
HhaI 719 GCGC GCG^C
HinP1I 719 GCGC G^CGC
Ksp632I 213 CTCTTC CTCTTC(1/4)
MaeII 119 ACGT A^CGT
MluNI 425 TGGCCA TGG^CCA
MscI 425 TGGCCA TGG^CCA
MspA1I 697 CAGCGG C[AC]G^C[GT]G
NdeI 369 CATATG CA^TATG
PpuMI 433 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 398 TCATGA T^CATGA
RsaI 320 GTAC GT^AC
StyI 312 CCTAGG C^C[AT][AT]GG
TaqI 153 TCGA T^CGA
TthHB8I 153 TCGA T^CGA
XcmI 194 CCATCTTTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AluI 135 AGCT AG^CT
293 AGCT
BseRI 87 CTCCTC GAGGAG(10/8)
144 CTCCTC
BsiYI 493 CCTTACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
504 CCCTAACAAGG
BslI 493 CCTTACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
504 CCCTAACAAGG
BsmFI 433 GGGAC GGGAC(10/14)
288 GTCCC
BssKI 204 CCTGG ^CC[ACGT]GG
437 CCTGG
BstNI 204 CCTGG CC^[AT]GG
437 CCTGG
DpnII 465 GATC ^GATC
886 GATC
DraII 56 AGGGCCT [GA]G^G[ACGT]CC[CT]
433 GGGACCT
EcoO109I 56 AGGGCCT [GA]G^G[ACGT]CC[CT]
433 GGGACCT
EcoRII 204 CCTGG ^CC[AT]GG
437 CCTGG
HinfI 41 GAATC G^A[ACGT]TC
843 GAATC
MboI 465 GATC ^GATC
886 GATC
MboII 214 TCTTC GAAGA(8/7)
299 TCTTC
MvaI 204 CCTGG CC^[AT]GG
437 CCTGG
NdeII 465 GATC ^GATC
886 GATC
NlaIV 433 GGGACC GG[ACGT]^[ACGT]CC
799 GGTTCC
PstI 539 CTGCAG CTGCA^G
714 CTGCAG
Sau3AI 465 GATC ^GATC
886 GATC
ScrFI 204 CCTGG CC^[ACGT]GG
437 CCTGG
TfiI 41 GAATC G^A[AT]TC
843 GAATC
Tsp45I 19 GTGAC ^GT[GC]AC
353 GTGAC
Tsp509I 26 AATT ^AATT
807 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI BbvI BfaI BsaJI BsmAI
BsrI BstF5I Cac8I Cfr13I DdeI
Fnu4HI FokI HaeIII HphI ItaI
MaeI MaeIII MnlI MslI NlaIII
Sau96I SfcI TseI TspRI