Restriction map for
pFN21AE0843
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BcgI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MfeI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 186 AAATTC [GA]^AATT[CT]
ApaI 527 GGGCCC GGGCC^C
ApoI 186 AAATTC [GA]^AATT[CT]
AspHI 837 GAGCTC G[AT]GC[AT]^C
BamHI 176 GGATCC G^GATCC
BbsI 727 GTCTTC GAAGAC(2/6)
BbvCI 405 CCTCAGC CCTCAGC(-5/-2)
BfaI 106 CTAG C^TAG
BpmI 167 CTGGAG CTGGAG(16/14)
BpuAI 727 GTCTTC GAAGAC(2/6)
BsaBI 471 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 240 GAGACC GGTCTC(1/5)
BseRI 517 CTCCTC GAGGAG(10/8)
BsiHKAI 837 GAGCTC G[AT]GC[AT]^C
BsiYI 519 CCTCTGCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 519 CCTCTGCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 546 GAATGC GAATGC(1/-1)
BspHI 977 TCATGA T^CATGA
BspMI 464 GCAGGT ACCTGC(4/8)
BstAPI 410 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 904 CCAGGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 298 TTTAAA TTT^AAA
DraII 527 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 382 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 527 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 548 ATGCAT ATGCA^T
HphI 834 GGTGA GGTGA(8/7)
MaeI 106 CTAG C^TAG
MaeIII 919 GTGAC ^GT[ACGT]AC
MamI 471 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 819 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 410 GCACCATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 548 ATGCAT ATGCA^T
NspI 657 ACATGC [GA]CATG^[CT]
PleI 779 GACTC GAGTC(4/5)
RcaI 977 TCATGA T^CATGA
SacI 837 GAGCTC GAGCT^C
SbfI 1013 CCTGCAGG CCTGCA^GG
Sse8387I 1013 CCTGCAGG CCTGCA^GG
SspI 338 AATATT AAT^ATT
Tsp45I 919 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 10 enzymes) #
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AvaII 288 GGTCC G^G[AT]CC
828 GGTCC
BanII 527 GGGCCC G[GA]GC[CT]^C
837 GAGCTC
BciVI 305 GGATAC GTATCC(6/5)
616 GGATAC
BstYI 176 GGATCC [GA]^GATC[CT]
487 AGATCC
Cac8I 955 GCACGC GC[ACGT]^[ACGT]GC
1012 GCCTGC
MflI 176 GGATCC [GA]^GATC[CT]
487 AGATCC
NcoI 284 CCATGG C^CATGG
722 CCATGG
PstI 786 CTGCAG CTGCA^G
1014 CTGCAG
SfaNI 550 GCATC GCATC(5/9)
634 GCATC
XhoII 176 GGATCC [GA]^GATC[CT]
487 AGATCC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AluI AlwI BbvI BmyI BsaJI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MboI MboII MnlI
MseI MvaI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfcI StyI
TfiI Tru9I TseI Tsp509I TspRI
XcmI