Restriction map for
pFN21AE0843
(Linear) (Six-base) MAPSORT of: KIEE0843.seq Check: 8015 from: 1 to: 1059
KIEE0843.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 455 1059
Size: 455 604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 1059
Size: 62 997
ApaI G_GGCC'C
Cuts at: 0 531 1059
Size: 531 528
ApoI r'AATT_y
Cuts at: 0 186 1059
Size: 186 873
BamHI G'GATC_C
Cuts at: 0 176 1059
Size: 176 883
BanII G_rGCy'C
Cuts at: 0 531 841 1059
Size: 531 310 218
Fragments arranged by size:
531 310 218
BbsI GAAGACnn'nnnn_
Cuts at: 0 720 1059
Size: 720 339
BbvCI CC'TCA_GC
Cuts at: 0 406 1059
Size: 406 653
BciVI GTATCCnnnnn_n'
Cuts at: 0 299 610 1059
Size: 299 311 449
Fragments arranged by size:
449 311 299
BfrBI ATG'CAT
Cuts at: 0 550 1059
Size: 550 509
Bme1580I G_kGCm'C
Cuts at: 0 394 531 1059
Size: 394 137 528
Fragments arranged by size:
528 394 137
BmrI ACTGGGnnnn_n'
Cuts at: 0 449 1059
Size: 449 610
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 421 1004 1059
Size: 421 583 55
Fragments arranged by size:
583 421 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 1059
Size: 188 871
Bpu10I CC'TnA_GC
Cuts at: 0 406 1059
Size: 406 653
BsaI GGTCTCn'nnnn_
Cuts at: 0 234 1059
Size: 234 825
BsaBI GATnn'nnATC
Cuts at: 0 475 1059
Size: 475 584
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 766 796 1059
Size: 766 30 263
Fragments arranged by size:
766 263 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 508 1059
Size: 508 551
BseYI C'CCAG_C
Cuts at: 0 524 951 1059
Size: 524 427 108
Fragments arranged by size:
524 427 108
BsiHKAI G_wGCw'C
Cuts at: 0 841 1059
Size: 841 218
BsmI GAATG_Cn'
Cuts at: 0 552 1059
Size: 552 507
Bsp1286I G_dGCh'C
Cuts at: 0 394 531 841 1059
Size: 394 137 310 218
Fragments arranged by size:
394 310 218 137
BspHI T'CATG_A
Cuts at: 0 977 1059
Size: 977 82
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 455 1059
Size: 455 604
BstAPI GCAn_nnn'nTGC
Cuts at: 0 416 1059
Size: 416 643
BstXI CCAn_nnnn'nTGG
Cuts at: 0 911 1059
Size: 911 148
BstYI r'GATC_y
Cuts at: 0 176 487 1059
Size: 176 311 572
Fragments arranged by size:
572 311 176
BtgI C'CryG_G
Cuts at: 0 245 284 722 1059
Size: 245 39 438 337
Fragments arranged by size:
438 337 245 39
DraI TTT'AAA
Cuts at: 0 300 1059
Size: 300 759
DraIII CAC_nnn'GTG
Cuts at: 0 387 1059
Size: 387 672
EcoICRI GAG'CTC
Cuts at: 0 839 1059
Size: 839 220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 188 1059
Size: 62 126 871
Fragments arranged by size:
871 126 62
EcoO109I rG'GnC_Cy
Cuts at: 0 528 1059
Size: 528 531
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 387 1059
Size: 387 672
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 161 193 1059
Size: 161 32 866
Fragments arranged by size:
866 161 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 783 1059
Size: 783 276
MslI CAynn'nnrTG
Cuts at: 0 823 1059
Size: 823 236
NcoI C'CATG_G
Cuts at: 0 284 722 1059
Size: 284 438 337
Fragments arranged by size:
438 337 284
NsiI A_TGCA'T
Cuts at: 0 552 1059
Size: 552 507
NspI r_CATG'y
Cuts at: 0 661 1059
Size: 661 398
PsiI TTA'TAA
Cuts at: 0 209 1059
Size: 209 850
PspOMI G'GGCC_C
Cuts at: 0 527 1059
Size: 527 532
PstI C_TGCA'G
Cuts at: 0 790 1018 1059
Size: 790 228 41
Fragments arranged by size:
790 228 41
SacI G_AGCT'C
Cuts at: 0 841 1059
Size: 841 218
SbfI CC_TGCA'GG
Cuts at: 0 1018 1059
Size: 1018 41
SfcI C'TryA_G
Cuts at: 0 738 786 890 1014 1059
Size: 738 48 104 124 45
Fragments arranged by size:
738 124 104 48 45
SspI AAT'ATT
Cuts at: 0 340 1059
Size: 340 719
StyI C'CwwG_G
Cuts at: 0 272 284 493 722 1059
Size: 272 12 209 229 337
Fragments arranged by size:
337 272 229 209 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 720 828 930 1059
Size: 720 108 102 129
Fragments arranged by size:
720 129 108 102
Enzymes that do cut:
AarI AcuI ApaI ApoI BamHI BanII BbsI BbvCI
BciVI BfrBI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaBI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI
BspMI BstAPI BstXI BstYI BtgI DraI DraIII EcoICRI
Eco57MI EcoO109I FalI Hin4I MmeI MslI NcoI NsiI
NspI PsiI PspOMI PstI SacI SbfI SfcI SspI
StyI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BcgI BclI
BglI BglII BlpI BmgBI BmtI BpuEI BsaAI BsaHI
BsaWI BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DrdI EaeI EagI EarI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI