(Circular) (Six-base) MAPSORT of: pFN21AE0836.seq Check: 2303 from: 1 to: 5659 pFN21AE0836 5659 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3469 278 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3002 1937 Size: 1065 4594 Acc65I G'GTAC_C Cuts at: 2980 2980 Size: 5659 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2425 2881 2945 3722 5106 5374 1632 Size: 793 456 64 777 1384 268 1917 Fragments arranged by size: 1917 1384 793 777 456 268 64 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 4558 5535 1203 Size: 3355 977 1327 Fragments arranged by size: 3355 1327 977 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5659 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2663 1184 Size: 1479 4180 AlwNI CAG_nnn'CTG Cuts at: 2904 3402 4974 2904 Size: 498 1572 3589 Fragments arranged by size: 3589 1572 498 ApaLI G'TGCA_C Cuts at: 3716 4872 3716 Size: 1156 4503 ApoI r'AATT_y Cuts at: 1493 2576 2834 2968 3194 3346 5455 1493 Size: 1083 258 134 226 152 2109 1697 Fragments arranged by size: 2109 1697 1083 258 226 152 134 AseI AT'TA_AT Cuts at: 160 1032 4213 160 Size: 872 3181 1606 Fragments arranged by size: 3181 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2984 1870 Size: 73 1041 4545 Fragments arranged by size: 4545 1041 73 AvrII C'CTAG_G Cuts at: 2188 2188 Size: 5659 BamHI G'GATC_C Cuts at: 2989 3356 2989 Size: 367 5292 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2980 4435 618 Size: 325 217 627 1193 1455 1842 Fragments arranged by size: 1842 1455 1193 627 325 217 BanII G_rGCy'C Cuts at: 1391 2457 2715 2978 1391 Size: 1066 258 263 4072 Fragments arranged by size: 4072 1066 263 258 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2344 2383 2674 928 Size: 971 445 39 291 3913 Fragments arranged by size: 3913 971 445 291 39 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3850 3884 1903 Size: 34 1913 34 3678 Fragments arranged by size: 3678 1913 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3551 4761 3551 Size: 1210 4449 BclI T'GATC_A Cuts at: 1548 1548 Size: 5659 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4270 136 Size: 107 122 71 631 605 2598 1525 Fragments arranged by size: 2598 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5654 5654 Size: 5659 BlpI GC'TnA_GC Cuts at: 3076 3076 Size: 5659 Bme1580I G_kGCm'C Cuts at: 1790 3720 4876 1790 Size: 1930 1156 2573 Fragments arranged by size: 2573 1930 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2709 3390 4348 Size: 416 53 83 399 24 1292 681 958 Cuts at: 4348 442 Size: 1753 Fragments arranged by size: 1753 1292 958 681 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2443 4319 1239 Size: 1204 1876 2579 Fragments arranged by size: 2579 1876 1204 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2926 3784 4649 4947 2926 Size: 858 865 298 3638 Fragments arranged by size: 3638 865 858 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2007 4322 882 Size: 617 508 2315 2219 Fragments arranged by size: 2315 2219 617 508 BsaAI yAC'GTr Cuts at: 493 1204 3450 493 Size: 711 2246 2702 Fragments arranged by size: 2702 2246 711 BsaBI GATnn'nnATC Cuts at: 5653 5653 Size: 5659 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3466 3848 4483 275 Size: 53 83 186 936 1933 382 635 1451 Fragments arranged by size: 1933 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 4490 4764 4911 5322 4092 Size: 398 274 147 411 4429 Fragments arranged by size: 4429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2554 2584 1178 Size: 30 165 30 1151 30 4253 Fragments arranged by size: 4253 1151 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2741 1186 Size: 1555 4104 BseYI C'CCAG_C Cuts at: 4862 5358 4862 Size: 496 5163 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2741 2889 1163 Size: 573 1005 148 3933 Fragments arranged by size: 3933 1005 573 148 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 3870 4898 1540 Size: 275 186 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3720 3805 4876 3720 Size: 85 1071 4503 Fragments arranged by size: 4503 1071 85 BsmI GAATG_Cn' Cuts at: 2320 3170 3263 2320 Size: 850 93 4716 Fragments arranged by size: 4716 850 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2457 2715 2978 3720 3805 4876 1391 Size: 399 667 258 263 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 667 399 263 258 85 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 3548 5278 3548 Size: 1730 3929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2798 3014 844 Size: 1954 216 3489 Fragments arranged by size: 3489 1954 216 BsrBI CCG'CTC Cuts at: 1394 1823 3546 1394 Size: 429 1723 3507 Fragments arranged by size: 3507 1723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2113 4154 4328 65 Size: 1183 865 2041 174 1396 Fragments arranged by size: 2041 1396 1183 865 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4303 4490 1700 Size: 110 2493 187 2869 Fragments arranged by size: 2869 2493 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5659 BstBI TT'CG_AA Cuts at: 4463 4463 Size: 5659 BstEII G'GTnAC_C Cuts at: 1184 2065 1184 Size: 881 4778 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2062 Size: 5659 BstYI r'GATC_y Cuts at: 1972 2030 2989 3356 3741 3758 5210 5296 5308 Size: 58 959 367 385 17 1452 86 12 Cuts at: 5308 5654 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 959 385 367 346 86 58 17 12 Bsu36I CC'TnA_GG Cuts at: 2698 2698 Size: 5659 BtgI C'CryG_G Cuts at: 513 1065 2429 513 Size: 552 1364 3743 Fragments arranged by size: 3743 1364 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4920 Fragments arranged by size: 4920 683 56 BtsI GCAGTG_nn' Cuts at: 2118 2619 3175 3969 3989 2118 Size: 501 556 794 20 3788 Fragments arranged by size: 3788 794 556 501 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2833 2963 2833 Size: 130 5529 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 809 Size: 507 3350 1802 Fragments arranged by size: 3350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3106 3995 5624 Size: 54 1425 50 275 18 1276 889 1629 Cuts at: 5624 8 Size: 43 Fragments arranged by size: 1629 1425 1276 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2444 2591 2774 2900 2921 3589 1353 Size: 1091 147 183 126 21 668 3423 Fragments arranged by size: 3423 1091 668 183 147 126 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4246 4620 4766 387 Size: 1064 138 2657 374 146 1280 Fragments arranged by size: 2657 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2425 2443 2881 2945 3722 4319 5106 Size: 393 793 18 438 64 777 597 787 Cuts at: 5106 5374 1239 Size: 268 1524 Fragments arranged by size: 1524 793 787 777 597 438 393 268 64 18 EcoO109I rG'GnC_Cy Cuts at: 2193 2193 Size: 5659 EcoRI G'AATT_C Cuts at: 2968 3346 2968 Size: 378 5281 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 4806 1678 Size: 3128 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4300 4332 4374 4406 1373 Size: 32 135 32 2728 32 42 32 2626 Fragments arranged by size: 2728 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3003 3249 669 Size: 988 281 1065 246 3079 Fragments arranged by size: 3079 1065 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5659 HpaI GTT'AAC Cuts at: 3249 3249 Size: 5659 KpnI G_GTAC'C Cuts at: 2984 2984 Size: 5659 MfeI C'AATT_G Cuts at: 3258 3258 Size: 5659 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4773 4957 1305 Size: 3468 184 2007 Fragments arranged by size: 3468 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3837 Fragments arranged by size: 3837 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2639 3617 3976 4135 518 Size: 648 1473 978 359 159 2042 Fragments arranged by size: 2042 1473 978 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3075 3752 4900 5145 1606 Size: 1469 677 1148 245 2120 Fragments arranged by size: 2120 1469 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2429 513 Size: 552 1364 3743 Fragments arranged by size: 3743 1364 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 3017 4562 1132 Size: 97 1788 1545 2229 Fragments arranged by size: 2229 1788 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5659 PciI A'CATG_T Cuts at: 4558 4558 Size: 5659 PflMI CCAn_nnn'nTGG Cuts at: 1108 2328 1108 Size: 1220 4439 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2920 2952 3728 3760 5241 5273 2920 Size: 32 776 32 1481 32 3306 Fragments arranged by size: 3306 1481 776 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5659 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PstI C_TGCA'G Cuts at: 830 2214 2286 2809 2909 3011 830 Size: 1384 72 523 100 102 3478 Fragments arranged by size: 3478 1384 523 102 100 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 SalI G'TCGA_C Cuts at: 1936 3001 1936 Size: 1065 4594 SapI GCTCTTCn'nnn_ Cuts at: 2444 2774 2900 2444 Size: 330 126 5203 Fragments arranged by size: 5203 330 126 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 2210 2282 2448 2805 2905 3007 4142 Size: 219 1165 72 166 357 100 102 1135 Cuts at: 4142 4823 5014 826 Size: 681 191 1471 Fragments arranged by size: 1471 1165 1135 681 357 219 191 166 102 100 72 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2986 1872 Size: 1114 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2941 3763 4664 4926 820 Size: 197 926 998 822 901 262 1553 Fragments arranged by size: 1553 998 926 901 822 262 197 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 3017 1132 Size: 1885 3774 SspI AAT'ATT Cuts at: 5 52 3583 5 Size: 47 3531 2081 Fragments arranged by size: 3531 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5659 StyI C'CwwG_G Cuts at: 513 1065 2188 2429 2534 3098 513 Size: 552 1123 241 105 564 3074 Fragments arranged by size: 3074 1123 564 552 241 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2131 2396 2409 2830 3686 3703 3856 Size: 1255 551 265 13 421 856 17 153 Cuts at: 3856 4041 4486 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 856 551 445 421 265 185 153 17 13 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2278 3905 5617 Size: 274 80 33 51 1443 301 1627 1712 Cuts at: 5617 96 Size: 138 Fragments arranged by size: 1712 1627 1443 301 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2022 2054 2554 2586 5619 5651 2022 Size: 32 500 32 3033 32 2030 Fragments arranged by size: 3033 2030 500 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2995 2995 Size: 5659 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2984 1870 Size: 1114 4545 XmnI GAAnn'nnTTC Cuts at: 3788 3788 Size: 5659 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3467 276 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI