(Circular) (Six-base) MAPSORT of: pFN21AE0823.seq Check: 5897 from: 1 to: 5404 pFN21AE0823 5404 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2687 2687 Size: 5404 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3214 278 Size: 53 83 186 936 1678 2468 Fragments arranged by size: 2468 1678 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2747 1937 Size: 810 4594 Acc65I G'GTAC_C Cuts at: 2420 2725 2420 Size: 305 5099 AclI AA'CG_TT Cuts at: 1563 3531 3904 1563 Size: 1968 373 3063 Fragments arranged by size: 3063 1968 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2579 3467 4851 5119 1632 Size: 947 888 1384 268 1917 Fragments arranged by size: 1917 1384 947 888 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5207 AflIII A'CryG_T Cuts at: 1203 4303 5280 1203 Size: 3100 977 1327 Fragments arranged by size: 3100 1327 977 AgeI A'CCGG_T Cuts at: 4235 4235 Size: 5404 AhdI GACnn_n'nnGTC Cuts at: 4133 4133 Size: 5404 AleI CACnn'nnGTG Cuts at: 1166 2177 1166 Size: 1011 4393 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2408 1184 Size: 1224 4180 AlwNI CAG_nnn'CTG Cuts at: 2248 3147 4719 2248 Size: 899 1572 2933 Fragments arranged by size: 2933 1572 899 ApaLI G'TGCA_C Cuts at: 2488 3461 4617 2488 Size: 973 1156 3275 Fragments arranged by size: 3275 1156 973 ApoI r'AATT_y Cuts at: 1493 2713 2939 3091 5200 1493 Size: 1220 226 152 2109 1697 Fragments arranged by size: 2109 1697 1220 226 152 AseI AT'TA_AT Cuts at: 160 1032 3958 160 Size: 872 2926 1606 Fragments arranged by size: 2926 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5404 AvaI C'yCGr_G Cuts at: 1870 1943 2729 1870 Size: 73 786 4545 Fragments arranged by size: 4545 786 73 BamHI G'GATC_C Cuts at: 2734 3101 2734 Size: 367 5037 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2420 2725 4180 618 Size: 325 217 627 633 305 1455 1842 Fragments arranged by size: 1842 1455 633 627 325 305 217 BanII G_rGCy'C Cuts at: 1391 2723 1391 Size: 1332 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2044 928 Size: 971 145 4288 Fragments arranged by size: 4288 971 145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3595 3629 1903 Size: 34 1658 34 3678 Fragments arranged by size: 3678 1658 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3296 4506 3296 Size: 1210 4194 BclI T'GATC_A Cuts at: 1548 2139 1548 Size: 591 4813 BfrBI ATG'CAT Cuts at: 2619 2619 Size: 5404 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2258 4015 136 Size: 107 122 71 631 605 586 1757 1525 Fragments arranged by size: 1757 1525 631 605 586 122 107 71 BglII A'GATC_T Cuts at: 5399 5399 Size: 5404 BlpI GC'TnA_GC Cuts at: 2821 2821 Size: 5404 Bme1580I G_kGCm'C Cuts at: 1790 2492 3465 4621 1790 Size: 702 973 1156 2573 Fragments arranged by size: 2573 1156 973 702 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5404 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3135 4093 442 Size: 416 53 83 399 24 1718 958 1753 Fragments arranged by size: 1753 1718 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5404 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2400 4064 1239 Size: 1161 1664 2579 Fragments arranged by size: 2579 1664 1161 Bpu10I CC'TnA_GC Cuts at: 2192 2192 Size: 5404 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3529 4394 4692 3529 Size: 865 298 4241 Fragments arranged by size: 4241 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4067 882 Size: 617 2568 2219 Fragments arranged by size: 2568 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2487 3195 493 Size: 711 1283 708 2702 Fragments arranged by size: 2702 1283 711 708 BsaBI GATnn'nnATC Cuts at: 5398 5398 Size: 5404 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3211 3593 4228 275 Size: 53 83 186 936 1678 382 635 1451 Fragments arranged by size: 1678 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3837 4235 4509 4656 5067 3837 Size: 398 274 147 411 4174 Fragments arranged by size: 4174 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2398 2428 1178 Size: 30 165 30 995 30 4154 Fragments arranged by size: 4154 995 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5404 BseYI C'CCAG_C Cuts at: 4607 5103 4607 Size: 496 4908 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2321 2346 2378 1163 Size: 573 585 25 32 4189 Fragments arranged by size: 4189 585 573 32 25 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2854 3615 4643 1540 Size: 275 186 853 761 1028 2301 Fragments arranged by size: 2301 1028 853 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2492 3465 3550 4621 2492 Size: 973 85 1071 3275 Fragments arranged by size: 3275 1071 973 85 BsmI GAATG_Cn' Cuts at: 2915 3008 2915 Size: 93 5311 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2492 2723 3465 3550 4621 1391 Size: 399 702 231 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 702 399 231 85 BspEI T'CCGG_A Cuts at: 5067 5067 Size: 5404 BspHI T'CATG_A Cuts at: 3293 5023 3293 Size: 1730 3674 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2352 2432 2687 2759 844 Size: 1508 80 255 72 3489 Fragments arranged by size: 3489 1508 255 80 72 BsrBI CCG'CTC Cuts at: 1394 1823 3291 1394 Size: 429 1468 3507 Fragments arranged by size: 3507 1468 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3899 4073 65 Size: 1183 2651 174 1396 Fragments arranged by size: 2651 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4048 4235 1700 Size: 110 2238 187 2869 Fragments arranged by size: 2869 2238 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5404 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5404 BssSI C'ACGA_G Cuts at: 3464 4476 3464 Size: 1012 4392 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5404 BstBI TT'CG_AA Cuts at: 4208 4208 Size: 5404 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5404 BstXI CCAn_nnnn'nTGG Cuts at: 2638 2638 Size: 5404 BstYI r'GATC_y Cuts at: 1972 2734 3101 3486 3503 4955 5041 5053 5399 Size: 762 367 385 17 1452 86 12 346 Cuts at: 5399 1972 Size: 1977 Fragments arranged by size: 1977 1452 762 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3913 Fragments arranged by size: 3913 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4665 Fragments arranged by size: 4665 683 56 BtsI GCAGTG_nn' Cuts at: 2390 2920 3714 3734 2390 Size: 530 794 20 4060 Fragments arranged by size: 4060 794 530 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5404 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5369 DraI TTT'AAA Cuts at: 2708 2708 Size: 5404 DraIII CAC_nnn'GTG Cuts at: 2337 2337 Size: 5404 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4411 809 Size: 507 3095 1802 Fragments arranged by size: 3095 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2250 2259 Size: 54 1425 50 275 18 177 243 9 Cuts at: 2259 2439 2851 3740 5369 8 Size: 180 412 889 1629 43 Fragments arranged by size: 1629 1425 889 412 275 243 180 177 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2851 1537 Size: 275 1039 4090 Fragments arranged by size: 4090 1039 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 3334 1353 Size: 737 1244 3423 Fragments arranged by size: 3423 1244 737 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3991 4365 4511 387 Size: 1064 138 2402 374 146 1280 Fragments arranged by size: 2402 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2400 2579 3467 4064 4851 5119 1239 Size: 393 768 179 888 597 787 268 1524 Fragments arranged by size: 1524 888 787 768 597 393 268 179 EcoRI G'AATT_C Cuts at: 2713 3091 2713 Size: 378 5026 FspI TGC'GCA Cuts at: 3910 3910 Size: 5404 HaeII r_GCGC'y Cuts at: 1678 4551 1678 Size: 2873 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2398 2430 4045 4077 4119 Size: 32 135 32 826 32 1615 32 42 Cuts at: 4119 4151 1373 Size: 32 2626 Fragments arranged by size: 2626 1615 826 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2748 2994 669 Size: 988 281 810 246 3079 Fragments arranged by size: 3079 988 810 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5404 HpaI GTT'AAC Cuts at: 2994 2994 Size: 5404 KpnI G_GTAC'C Cuts at: 2424 2729 2424 Size: 305 5099 MfeI C'AATT_G Cuts at: 3003 3003 Size: 5404 MluI A'CGCG_T Cuts at: 5280 5280 Size: 5404 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4518 4702 1305 Size: 3213 184 2007 Fragments arranged by size: 3213 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2252 2261 2441 10 Size: 54 1425 343 177 243 9 180 2973 Fragments arranged by size: 2973 1425 343 243 180 177 54 9 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2408 3362 3721 3880 518 Size: 648 1011 231 954 359 159 2042 Fragments arranged by size: 2042 1011 954 648 359 231 159 MspA1I CmG'CkG Cuts at: 1606 2820 3497 4645 4890 1606 Size: 1214 677 1148 245 2120 Fragments arranged by size: 2120 1214 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3913 Fragments arranged by size: 3913 939 552 NdeI CA'TA_TG Cuts at: 387 2321 387 Size: 1934 3470 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5404 NotI GC'GGCC_GC Cuts at: 2851 2851 Size: 5404 NsiI A_TGCA'T Cuts at: 2621 2621 Size: 5404 NspI r_CATG'y Cuts at: 1132 1229 2623 2662 2762 4307 1132 Size: 97 1394 39 100 1545 2229 Fragments arranged by size: 2229 1545 1394 100 97 39 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5404 PciI A'CATG_T Cuts at: 4303 4303 Size: 5404 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5404 PfoI T'CCnGG_A Cuts at: 2208 2208 Size: 5404 PmeI GTTT'AAAC Cuts at: 2708 2708 Size: 5404 PmlI CAC'GTG Cuts at: 2487 2487 Size: 5404 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3473 3505 4986 5018 3473 Size: 32 1481 32 3859 Fragments arranged by size: 3859 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5404 PsiI TTA'TAA Cuts at: 2974 2974 Size: 5404 PstI C_TGCA'G Cuts at: 830 2429 2573 2756 830 Size: 1599 144 183 3478 Fragments arranged by size: 3478 1599 183 144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5404 SalI G'TCGA_C Cuts at: 1936 2746 1936 Size: 810 4594 SbfI CC_TGCA'GG Cuts at: 2429 2756 2429 Size: 327 5077 ScaI AGT'ACT Cuts at: 3652 5364 3652 Size: 1712 3692 SfcI C'TryA_G Cuts at: 826 1045 2425 2569 2752 3887 4568 4759 826 Size: 219 1380 144 183 1135 681 191 1471 Fragments arranged by size: 1471 1380 1135 681 219 191 183 144 SfiI GGCCn_nnn'nGGCC Cuts at: 2258 2258 Size: 5404 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5404 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5404 SmaI CCC'GGG Cuts at: 1872 2731 1872 Size: 859 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3508 4409 4671 820 Size: 197 926 1565 901 262 1553 Fragments arranged by size: 1565 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3195 493 Size: 2702 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5404 SphI G_CATG'C Cuts at: 1132 2762 1132 Size: 1630 3774 SspI AAT'ATT Cuts at: 5 52 3328 5 Size: 47 3276 2081 Fragments arranged by size: 3276 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5404 StyI C'CwwG_G Cuts at: 513 1065 2004 2843 513 Size: 552 939 839 3074 Fragments arranged by size: 3074 939 839 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3431 3448 3601 3786 4231 325 Size: 1255 1851 17 153 185 445 1498 Fragments arranged by size: 1851 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3650 5362 Size: 274 80 33 51 1443 204 1469 1712 Cuts at: 5362 96 Size: 138 Fragments arranged by size: 1712 1469 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5364 5396 5364 Size: 32 5372 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5404 XbaI T'CTAG_A Cuts at: 2740 2740 Size: 5404 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5404 XmaI C'CCGG_G Cuts at: 1870 2729 1870 Size: 859 4545 XmnI GAAnn'nnTTC Cuts at: 3533 3533 Size: 5404 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3212 276 Size: 53 83 186 936 1678 2468 Fragments arranged by size: 2468 1678 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI