Restriction map for
pFN21AE0823
(Linear) (Six-base) MAPSORT of: KIEE0823.seq Check: 9585 from: 1 to: 699
KIEE0823.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 682 699
Size: 682 17
Acc65I G'GTAC_C
Cuts at: 0 415 699
Size: 415 284
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 699
Size: 574 125
AleI CACnn'nnGTG
Cuts at: 0 172 699
Size: 172 527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 403 699
Size: 403 296
AlwNI CAG_nnn'CTG
Cuts at: 0 243 699
Size: 243 456
ApaLI G'TGCA_C
Cuts at: 0 483 699
Size: 483 216
BanI G'GyrC_C
Cuts at: 0 415 699
Size: 415 284
BbsI GAAGACnn'nnnn_
Cuts at: 0 39 699
Size: 39 660
BclI T'GATC_A
Cuts at: 0 134 699
Size: 134 565
BfrBI ATG'CAT
Cuts at: 0 614 699
Size: 614 85
BglI GCCn_nnn'nGGC
Cuts at: 0 253 699
Size: 253 446
Bme1580I G_kGCm'C
Cuts at: 0 487 699
Size: 487 212
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 699
Size: 395 304
Bpu10I CC'TnA_GC
Cuts at: 0 187 699
Size: 187 512
BsaAI yAC'GTr
Cuts at: 0 482 699
Size: 482 217
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 393 423 699
Size: 393 30 276
Fragments arranged by size:
393 276 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 341 373 699
Size: 316 25 32 326
Fragments arranged by size:
326 316 32 25
BsiHKAI G_wGCw'C
Cuts at: 0 487 699
Size: 487 212
Bsp1286I G_dGCh'C
Cuts at: 0 487 699
Size: 487 212
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 347 427 682 699
Size: 347 80 255 17
Fragments arranged by size:
347 255 80 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 633 699
Size: 633 66
BtsI GCAGTG_nn'
Cuts at: 0 385 699
Size: 385 314
DraIII CAC_nnn'GTG
Cuts at: 0 332 699
Size: 332 367
EaeI y'GGCC_r
Cuts at: 0 2 245 254 434 699
Size: 2 243 9 180 265
Fragments arranged by size:
265 243 180 9 2
EarI CTCTTCn'nnn_
Cuts at: 0 85 699
Size: 85 614
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 574 699
Size: 395 179 125
Fragments arranged by size:
395 179 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 393 425 699
Size: 393 32 274
Fragments arranged by size:
393 274 32
KpnI G_GTAC'C
Cuts at: 0 419 699
Size: 419 280
MscI TGG'CCA
Cuts at: 0 4 247 256 436 699
Size: 4 243 9 180 263
Fragments arranged by size:
263 243 180 9 4
MslI CAynn'nnrTG
Cuts at: 0 172 403 699
Size: 172 231 296
Fragments arranged by size:
296 231 172
NdeI CA'TA_TG
Cuts at: 0 316 699
Size: 316 383
NsiI A_TGCA'T
Cuts at: 0 616 699
Size: 616 83
NspI r_CATG'y
Cuts at: 0 618 657 699
Size: 618 39 42
Fragments arranged by size:
618 42 39
PfoI T'CCnGG_A
Cuts at: 0 203 699
Size: 203 496
PmlI CAC'GTG
Cuts at: 0 482 699
Size: 482 217
PstI C_TGCA'G
Cuts at: 0 424 568 699
Size: 424 144 131
Fragments arranged by size:
424 144 131
SbfI CC_TGCA'GG
Cuts at: 0 424 699
Size: 424 275
SfcI C'TryA_G
Cuts at: 0 420 564 699
Size: 420 144 135
Fragments arranged by size:
420 144 135
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 253 699
Size: 253 446
TatI w'GTAC_w
Cuts at: 0 176 699
Size: 176 523
Enzymes that do cut:
AarI Acc65I AcuI AleI AlfI AlwNI ApaLI BanI
BbsI BclI BfrBI BglI Bme1580I BpmI Bpu10I BsaAI
BsaXI BsgI BsiHKAI Bsp1286I BspMI BstXI BtsI DraIII
EaeI EarI Eco57MI Hin4I KpnI MscI MslI NdeI
NsiI NspI PfoI PmlI PstI SbfI SfcI SfiI
TatI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BciVI BglII
BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI MmeI MspA1I
NaeI NarI NcoI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI