Restriction map for
pFN21AE0821
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeII
MaeIII MamI MflI MluI MroI
MspA1I MspI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AluI 741 AGCT AG^CT
AlwI 693 GATCC GGATC(4/5)
AocI 268 CCTAAGG CC^T[ACGT]AGG
AspHI 571 GAGCAC G[AT]GC[AT]^C
AvaII 291 GGTCC G^G[AT]CC
BalI 380 TGGCCA TGG^CCA
BanII 173 GAGCCC G[GA]GC[CT]^C
BbvI 528 GCTGC GCAGC(8/12)
BfaI 184 CTAG C^TAG
BseRI 777 CTCCTC GAGGAG(10/8)
BsiHKAI 571 GAGCAC G[AT]GC[AT]^C
BsmAI 22 GAGAC GTCTC(1/5)
BsmI 667 GCATTC GAATGC(1/-1)
Bsp1407I 146 TGTACA T^GTACA
BsrGI 146 TGTACA T^GTACA
BsrI 102 ACTGG ACTGG(1/-1)
Bsu36I 268 CCTAAGG CC^T[ACGT]AGG
DrdI 604 GACATAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 383 CCATGG C^C[GA][CT]GG
EaeI 380 TGGCCA [CT]^GGCC[GA]
Eco81I 268 CCTAAGG CC^T[ACGT]AGG
EcoRV 241 GATATC GAT^ATC
Fnu4HI 528 GCTGC GC^[ACGT]GC
HphI 56 TCACC GGTGA(8/7)
ItaI 528 GCTGC GC^[ACGT]GC
MaeI 184 CTAG C^TAG
MfeI 589 CAATTG C^AATTG
MluNI 380 TGGCCA TGG^CCA
MscI 380 TGGCCA TGG^CCA
MunI 589 CAATTG C^AATTG
NcoI 383 CCATGG C^CATGG
NspI 38 GCATGT [GA]CATG^[CT]
PstI 155 CTGCAG CTGCA^G
SfcI 155 CTGCAG C^T[GA][CT]AG
SfiI 585 GGCCCAATTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 420 CTTGAG C^T[CT][GA]AG
SspBI 146 TGTACA T^GTACA
StuI 272 AGGCCT AGG^CCT
TfiI 371 GAATC G^A[AT]TC
TseI 528 GCTGC G^C[AT]GC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 147 GTAC GT^AC
413 GTAC
BbsI 11 GTCTTC GAAGAC(2/6)
214 GTCTTC
BglI 352 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
586 GCCCAATTGGC
BmyI 173 GAGCCC G[AGT]GC[ACT]^C
571 GAGCAC
BpmI 697 CTCCAG CTGGAG(16/14)
103 CTGGAG
BpuAI 11 GTCTTC GAAGAC(2/6)
214 GTCTTC
Bsp1286I 173 GAGCCC G[AGT]GC[ACT]^C
571 GAGCAC
Cfr13I 291 GGTCC G^G[ACGT]CC
585 GGCCC
DpnII 282 GATC ^GATC
693 GATC
EarI 780 CTCTTC CTCTTC(1/4)
522 GAAGAG
Ksp632I 780 CTCTTC CTCTTC(1/4)
522 GAAGAG
MboI 282 GATC ^GATC
693 GATC
MseI 439 TTAA T^TAA
746 TTAA
MslI 258 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
576 CATTGGGGTG
NdeII 282 GATC ^GATC
693 GATC
NlaIV 172 GGAGCC GG[ACGT]^[ACGT]CC
290 GGGTCC
RsaI 147 GTAC GT^AC
413 GTAC
Sau3AI 282 GATC ^GATC
693 GATC
Sau96I 291 GGTCC G^G[ACGT]CC
585 GGCCC
Tru9I 439 TTAA T^TAA
746 TTAA
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
BsaJI BsiYI BslI BsmFI BssKI
BstF5I BstNI Cac8I DdeI EcoRII
EcoT14I FokI HaeIII HinfI MboII
MnlI MvaI MwoI NlaIII PleI
ScrFI SfaNI StyI Tsp509I TspRI