(Circular) (Six-base) MAPSORT of: pFN21AE0821.seq Check: 1228 from: 1 to: 5515 pFN21AE0821 5515 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3325 278 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2858 1937 Size: 921 4594 Acc65I G'GTAC_C Cuts at: 2836 2836 Size: 5515 AclI AA'CG_TT Cuts at: 1563 3642 4015 1563 Size: 2079 373 3063 Fragments arranged by size: 3063 2079 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2546 3578 4962 5230 1632 Size: 914 1032 1384 268 1917 Fragments arranged by size: 1917 1384 1032 914 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5318 AflIII A'CryG_T Cuts at: 1203 4414 5391 1203 Size: 3211 977 1327 Fragments arranged by size: 3211 1327 977 AgeI A'CCGG_T Cuts at: 4346 4346 Size: 5515 AhdI GACnn_n'nnGTC Cuts at: 4244 4244 Size: 5515 AleI CACnn'nnGTG Cuts at: 1166 2267 1166 Size: 1101 4414 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5515 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2196 2228 2196 Size: 32 5483 AlwNI CAG_nnn'CTG Cuts at: 3258 4830 3258 Size: 1572 3943 ApaLI G'TGCA_C Cuts at: 3572 4728 3572 Size: 1156 4359 ApoI r'AATT_y Cuts at: 1493 2824 3050 3202 5311 1493 Size: 1331 226 152 2109 1697 Fragments arranged by size: 2109 1697 1331 226 152 AseI AT'TA_AT Cuts at: 160 1032 4069 160 Size: 872 3037 1606 Fragments arranged by size: 3037 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 AvaI C'yCGr_G Cuts at: 1870 1943 2840 1870 Size: 73 897 4545 Fragments arranged by size: 4545 897 73 BamHI G'GATC_C Cuts at: 2845 3212 2845 Size: 367 5148 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2836 4291 618 Size: 325 217 627 1049 1455 1842 Fragments arranged by size: 1842 1455 1049 627 325 217 BanII G_rGCy'C Cuts at: 1391 2182 2834 1391 Size: 791 652 4072 Fragments arranged by size: 4072 791 652 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2009 2212 928 Size: 971 110 203 4231 Fragments arranged by size: 4231 971 203 110 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3706 3740 1903 Size: 34 1769 34 3678 Fragments arranged by size: 3678 1769 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3407 4617 3407 Size: 1210 4305 BclI T'GATC_A Cuts at: 1548 1548 Size: 5515 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2363 2597 4126 Size: 107 122 71 631 605 691 234 1529 Cuts at: 4126 136 Size: 1525 Fragments arranged by size: 1529 1525 691 631 605 234 122 107 71 BglII A'GATC_T Cuts at: 5510 5510 Size: 5515 BlpI GC'TnA_GC Cuts at: 2932 2932 Size: 5515 Bme1580I G_kGCm'C Cuts at: 1790 3576 4732 1790 Size: 1786 1156 2573 Fragments arranged by size: 2573 1786 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5515 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3246 4204 442 Size: 416 53 83 399 24 1829 958 1753 Fragments arranged by size: 1829 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5515 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2797 2797 Size: 5515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2687 4175 1239 Size: 890 558 1488 2579 Fragments arranged by size: 2579 1488 890 558 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2446 3640 4505 4803 2446 Size: 1194 865 298 3158 Fragments arranged by size: 3158 1194 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4178 882 Size: 617 2679 2219 Fragments arranged by size: 2679 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3306 493 Size: 711 2102 2702 Fragments arranged by size: 2702 2102 711 BsaBI GATnn'nnATC Cuts at: 5509 5509 Size: 5515 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3322 3704 4339 275 Size: 53 83 186 936 1789 382 635 1451 Fragments arranged by size: 1789 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3948 4346 4620 4767 5178 3948 Size: 398 274 147 411 4285 Fragments arranged by size: 4285 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2196 2226 1178 Size: 30 165 30 793 30 4467 Fragments arranged by size: 4467 793 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2773 1186 Size: 1587 3928 BseYI C'CCAG_C Cuts at: 2303 2736 4718 5214 2303 Size: 433 1982 496 2604 Fragments arranged by size: 2604 1982 496 433 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4942 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2965 3726 4754 1540 Size: 275 186 964 761 1028 2301 Fragments arranged by size: 2301 1028 964 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2580 3576 3661 4732 2580 Size: 996 85 1071 3363 Fragments arranged by size: 3363 1071 996 85 BsmI GAATG_Cn' Cuts at: 2672 3026 3119 2672 Size: 354 93 5068 Fragments arranged by size: 5068 354 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2182 2580 2834 3576 3661 4732 1391 Size: 399 392 398 254 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 398 392 254 85 BspEI T'CCGG_A Cuts at: 5178 5178 Size: 5515 BspHI T'CATG_A Cuts at: 3404 5134 3404 Size: 1730 3785 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2870 844 Size: 2026 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3402 1394 Size: 429 1579 3507 Fragments arranged by size: 3507 1579 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4010 4184 65 Size: 1183 2762 174 1396 Fragments arranged by size: 2762 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4159 4346 1700 Size: 110 2349 187 2869 Fragments arranged by size: 2869 2349 187 110 BsrGI T'GTAC_A Cuts at: 96 2151 96 Size: 2055 3460 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5515 BssSI C'ACGA_G Cuts at: 3575 4587 3575 Size: 1012 4503 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5515 BstBI TT'CG_AA Cuts at: 4319 4319 Size: 5515 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5515 BstYI r'GATC_y Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510 Size: 873 367 385 17 1452 86 12 346 Cuts at: 5510 1972 Size: 1977 Fragments arranged by size: 1977 1452 873 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2274 2274 Size: 5515 BtgI C'CryG_G Cuts at: 513 1065 2388 513 Size: 552 1323 3640 Fragments arranged by size: 3640 1323 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4776 Fragments arranged by size: 4776 683 56 BtsI GCAGTG_nn' Cuts at: 2157 3031 3825 3845 2157 Size: 874 794 20 3827 Fragments arranged by size: 3827 874 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5480 DraI TTT'AAA Cuts at: 2819 2819 Size: 5515 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2615 4522 809 Size: 507 1299 1907 1802 Fragments arranged by size: 1907 1802 1299 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2385 2962 3851 Size: 54 1425 50 275 18 555 577 889 Cuts at: 3851 5480 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 577 555 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2962 1537 Size: 275 1150 4090 Fragments arranged by size: 4090 1150 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2522 2791 3445 1353 Size: 1169 269 654 3423 Fragments arranged by size: 3423 1169 654 269 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4102 4476 4622 387 Size: 1064 138 2513 374 146 1280 Fragments arranged by size: 2513 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2546 2687 3578 4175 4962 5230 Size: 393 497 417 141 891 597 787 268 Cuts at: 5230 1239 Size: 1524 Fragments arranged by size: 1524 891 787 597 497 417 393 268 141 EcoRI G'AATT_C Cuts at: 2824 3202 2824 Size: 378 5137 EcoRV GAT'ATC Cuts at: 2248 2248 Size: 5515 FspI TGC'GCA Cuts at: 4021 4021 Size: 5515 HaeII r_GCGC'y Cuts at: 1678 4662 1678 Size: 2984 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2169 2201 4156 4188 4230 Size: 32 135 32 597 32 1955 32 42 Cuts at: 4230 4262 1373 Size: 32 2626 Fragments arranged by size: 2626 1955 597 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2859 3105 669 Size: 988 281 921 246 3079 Fragments arranged by size: 3079 988 921 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5515 HpaI GTT'AAC Cuts at: 3105 3105 Size: 5515 KpnI G_GTAC'C Cuts at: 2840 2840 Size: 5515 MfeI C'AATT_G Cuts at: 2594 3114 2594 Size: 520 4995 MluI A'CGCG_T Cuts at: 5391 5391 Size: 5515 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4629 4813 1305 Size: 3324 184 2007 Fragments arranged by size: 3324 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2387 10 Size: 54 1425 343 555 3138 Fragments arranged by size: 3138 1425 555 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2267 2585 3473 3832 3991 518 Size: 648 1101 318 888 359 159 2042 Fragments arranged by size: 2042 1101 888 648 359 318 159 MspA1I CmG'CkG Cuts at: 1606 2931 3608 4756 5001 1606 Size: 1325 677 1148 245 2120 Fragments arranged by size: 2120 1325 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2388 513 Size: 552 1323 3640 Fragments arranged by size: 3640 1323 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5515 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5515 NotI GC'GGCC_GC Cuts at: 2962 2962 Size: 5515 NspI r_CATG'y Cuts at: 1132 1229 2047 2873 4418 1132 Size: 97 818 826 1545 2229 Fragments arranged by size: 2229 1545 826 818 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5515 PciI A'CATG_T Cuts at: 4414 4414 Size: 5515 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5515 PmeI GTTT'AAAC Cuts at: 2819 2819 Size: 5515 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2196 2228 3584 3616 5097 5129 2196 Size: 32 1356 32 1481 32 2582 Fragments arranged by size: 2582 1481 1356 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5515 PsiI TTA'TAA Cuts at: 3085 3085 Size: 5515 PstI C_TGCA'G Cuts at: 830 2164 2867 830 Size: 1334 703 3478 Fragments arranged by size: 3478 1334 703 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5515 SalI G'TCGA_C Cuts at: 1936 2857 1936 Size: 921 4594 SbfI CC_TGCA'GG Cuts at: 2867 2867 Size: 5515 ScaI AGT'ACT Cuts at: 3763 5475 3763 Size: 1712 3803 SfcI C'TryA_G Cuts at: 826 1045 2160 2863 3998 4679 4870 826 Size: 219 1115 703 1135 681 191 1471 Fragments arranged by size: 1471 1135 1115 703 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2597 2597 Size: 5515 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5515 SmaI CCC'GGG Cuts at: 1872 2842 1872 Size: 970 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2425 3619 4520 4782 820 Size: 197 926 482 1194 901 262 1553 Fragments arranged by size: 1553 1194 926 901 482 262 197 SnaBI TAC'GTA Cuts at: 493 3306 493 Size: 2813 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5515 SphI G_CATG'C Cuts at: 1132 2873 1132 Size: 1741 3774 SspI AAT'ATT Cuts at: 5 52 3439 5 Size: 47 3387 2081 Fragments arranged by size: 3387 2081 47 StuI AGG'CCT Cuts at: 1517 2279 1517 Size: 762 4753 StyI C'CwwG_G Cuts at: 513 1065 2092 2280 2388 2954 513 Size: 552 1027 188 108 566 3074 Fragments arranged by size: 3074 1027 566 552 188 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3542 3559 3712 3897 4342 325 Size: 1255 1962 17 153 185 445 1498 Fragments arranged by size: 1962 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2151 2417 3761 Size: 274 80 33 51 1443 174 266 1344 Cuts at: 3761 5473 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1344 274 266 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2056 2088 5475 5507 2056 Size: 32 3387 32 2064 Fragments arranged by size: 3387 2064 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5515 XbaI T'CTAG_A Cuts at: 2851 2851 Size: 5515 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5515 XmaI C'CCGG_G Cuts at: 1870 2840 1870 Size: 970 4545 XmnI GAAnn'nnTTC Cuts at: 3644 3644 Size: 5515 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3323 276 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI