Restriction map for
pFN21AE0807
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI AlwI
AocI AscI AseI AsnI Asp700I
Asp718I AspEI AviII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfrI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MaeIII
MamI MfeI MluI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TaqI TfiI Tru9I Tsp45I
TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 154 GTCTAC GT^[AC][GT]AC
AccIII 780 TCCGGA T^CCGGA
AcsI 621 GAATTT [GA]^AATT[CT]
Aor51HI 242 AGCGCT AGC^GCT
ApaI 524 GGGCCC GGGCC^C
ApoI 621 GAATTT [GA]^AATT[CT]
AspI 657 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 585 TGGCCA TGG^CCA
BanI 508 GGCACC G^G[CT][GA]CC
BclI 723 TGATCA T^GATCA
BglI 902 GCCAGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1075 AGATCT A^GATCT
BsaI 121 GGTCTC GGTCTC(1/5)
BseAI 780 TCCGGA T^CCGGA
BsmAI 122 GTCTC GTCTC(1/5)
BsmFI 80 GGGAC GGGAC(10/14)
BspEI 780 TCCGGA T^CCGGA
BspHI 744 TCATGA T^CATGA
BstYI 1075 AGATCT [GA]^GATC[CT]
CfoI 243 GCGC GCG^C
DraII 524 GGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 242 AGCGCT AGC^GCT
EcoO109I 524 GGGCCCT [GA]G^G[ACGT]CC[CT]
FbaI 723 TGATCA T^GATCA
HaeII 242 AGCGCT [GA]GCGC^[CT]
HhaI 243 GCGC GCG^C
HinP1I 243 GCGC G^CGC
HinfI 995 GAGTC G^A[ACGT]TC
MflI 1075 AGATCT [GA]^GATC[CT]
MluNI 585 TGGCCA TGG^CCA
MroI 780 TCCGGA T^CCGGA
MscI 585 TGGCCA TGG^CCA
NspI 495 GCATGC [GA]CATG^[CT]
PflFI 657 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 995 GAGTC GAGTC(4/5)
RcaI 744 TCATGA T^CATGA
SacI 53 GAGCTC GAGCT^C
SfcI 171 CTACAG C^T[GA][CT]AG
SmaI 77 CCCGGG CCC^GGG
SphI 495 GCATGC GCATG^C
StuI 926 AGGCCT AGG^CCT
Tsp509I 622 AATT ^AATT
Tth111I 657 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1075 AGATCT [GA]^GATC[CT]
XmaCI 77 CCCGGG C^CCGGG
XmaI 77 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 489 CCGC CCGC(-3/-1)
38 GCGG
AfaI 144 GTAC GT^AC
967 GTAC
Alw44I 376 GTGCAC G^TGCAC
436 GTGCAC
AlwNI 541 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1009 CAGAAACTG
ApaLI 376 GTGCAC G^TGCAC
436 GTGCAC
AvaI 77 CCCGGG C^[CT]CG[GA]G
765 CCCGAG
AvrII 189 CCTAGG C^CTAGG
447 CCTAGG
BanII 53 GAGCTC G[GA]GC[CT]^C
524 GGGCCC
BcnI 77 CCCGG CC^[GC]GG
78 CCGGG
BlnI 189 CCTAGG C^CTAGG
447 CCTAGG
BsaWI 482 ACCGGA [AT]^CCGG[AT]
780 TCCGGA
BseRI 1003 CTCCTC GAGGAG(10/8)
734 GAGGAG
BsoBI 77 CCCGGG C^[CT]CG[GA]G
765 CCCGAG
BspMI 290 ACCTGC ACCTGC(4/8)
424 ACCTGC
BstXI 260 CCACCTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
786 CCATCCTACTGG
EaeI 284 TGGCCG [CT]^GGCC[GA]
585 TGGCCA
EarI 806 CTCTTC CTCTTC(1/4)
1053 GAAGAG
FauI 488 CCCGC CCCGC(4/6)
38 GCGGG
Ksp632I 806 CTCTTC CTCTTC(1/4)
1053 GAAGAG
MboII 1053 GAAGA GAAGA(8/7)
807 TCTTC
MslI 429 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
688 CATTTTGGTG
NciI 77 CCCGG CC^[GC]GG
78 CCGGG
PflMI 199 CCACTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
239 CCAAGCGCTGG
RsaI 144 GTAC GT^AC
967 GTAC
Van91I 199 CCACTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
239 CCAAGCGCTGG
XcmI 257 CCACCACCTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
518 CCACCTGGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AluI AspHI AvaII BbvI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MaeI
MboI MnlI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PvuII
Sau3AI Sau96I ScrFI SfaNI StyI
TseI TspRI