(Circular) (Six-base) MAPSORT of: pFN21AE0807.seq Check: 7343 from: 1 to: 5788 pFN21AE0807 5788 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3598 278 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2160 3131 1937 Size: 223 971 4594 Fragments arranged by size: 4594 971 223 Acc65I G'GTAC_C Cuts at: 3109 3109 Size: 5788 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3851 5235 5503 1632 Size: 2219 1384 268 1917 Fragments arranged by size: 2219 1917 1384 268 AfeI AGC'GCT Cuts at: 2249 2249 Size: 5788 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 4687 5664 1203 Size: 3484 977 1327 Fragments arranged by size: 3484 1327 977 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5788 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2396 2435 2438 2456 1184 Size: 1212 39 3 18 4516 Fragments arranged by size: 4516 1212 39 18 3 AlwNI CAG_nnn'CTG Cuts at: 2551 3019 3531 5103 2551 Size: 468 512 1572 3236 Fragments arranged by size: 3236 1572 512 468 ApaI G_GGCC'C Cuts at: 2533 2533 Size: 5788 ApaLI G'TGCA_C Cuts at: 2381 2441 3845 5001 2381 Size: 60 1404 1156 3168 Fragments arranged by size: 3168 1404 1156 60 ApoI r'AATT_y Cuts at: 1493 2626 3097 3323 3475 5584 1493 Size: 1133 471 226 152 2109 1697 Fragments arranged by size: 2109 1697 1133 471 226 152 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 2082 2770 3113 1870 Size: 73 139 688 343 4545 Fragments arranged by size: 4545 688 343 139 73 AvrII C'CTAG_G Cuts at: 2194 2452 2194 Size: 258 5530 BamHI G'GATC_C Cuts at: 3118 3485 3118 Size: 367 5421 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2513 3109 4564 618 Size: 325 217 627 726 596 1455 1842 Fragments arranged by size: 1842 1455 726 627 596 325 217 BanII G_rGCy'C Cuts at: 1391 2062 2533 3107 1391 Size: 671 471 574 4072 Fragments arranged by size: 4072 671 574 471 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4817 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3979 4013 1903 Size: 34 50 34 1958 34 3678 Fragments arranged by size: 3678 1958 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3680 4890 3680 Size: 1210 4578 BclI T'GATC_A Cuts at: 1548 2728 1548 Size: 1180 4608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2913 4399 136 Size: 107 122 71 631 605 1241 1486 1525 Fragments arranged by size: 1525 1486 1241 631 605 122 107 71 BglII A'GATC_T Cuts at: 3080 5783 3080 Size: 2703 3085 BlpI GC'TnA_GC Cuts at: 3205 3205 Size: 5788 Bme1580I G_kGCm'C Cuts at: 1790 2385 2445 2516 2533 2833 3849 5005 1790 Size: 595 60 71 17 300 1016 1156 2573 Fragments arranged by size: 2573 1156 1016 595 300 71 60 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5788 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2958 3519 4477 Size: 416 53 83 399 24 1541 561 958 Cuts at: 4477 442 Size: 1753 Fragments arranged by size: 1753 1541 958 561 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2081 2095 3023 2081 Size: 14 928 4846 Fragments arranged by size: 4846 928 14 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2272 2528 2996 4448 1239 Size: 851 182 256 468 1452 2579 Fragments arranged by size: 2579 1452 851 468 256 182 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3913 4778 5076 3913 Size: 865 298 4625 Fragments arranged by size: 4625 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2132 4451 882 Size: 617 633 2319 2219 Fragments arranged by size: 2319 2219 633 617 BsaAI yAC'GTr Cuts at: 493 1204 3579 493 Size: 711 2375 2702 Fragments arranged by size: 2702 2375 711 BsaBI GATnn'nnATC Cuts at: 5782 5782 Size: 5788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3595 3977 4612 275 Size: 53 83 186 936 2062 382 635 1451 Fragments arranged by size: 2062 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2487 2785 4221 4619 4893 5040 5451 2487 Size: 298 1436 398 274 147 411 2824 Fragments arranged by size: 2824 1436 411 398 298 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2038 2068 1178 Size: 30 165 30 635 30 4898 Fragments arranged by size: 4898 635 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2754 2999 1186 Size: 1568 245 3975 Fragments arranged by size: 3975 1568 245 BseYI C'CCAG_C Cuts at: 2509 3050 4991 5487 2509 Size: 541 1941 496 2810 Fragments arranged by size: 2810 1941 541 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5215 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3238 3999 5027 1540 Size: 275 186 1237 761 1028 2301 Fragments arranged by size: 2301 1237 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2062 2385 2445 3849 3934 5005 2062 Size: 323 60 1404 85 1071 2845 Fragments arranged by size: 2845 1404 1071 323 85 60 BsmI GAATG_Cn' Cuts at: 3299 3392 3299 Size: 93 5695 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2385 2445 2516 2533 2833 3107 Size: 399 272 323 60 71 17 300 274 Cuts at: 3107 3849 3934 5005 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 323 300 274 272 85 71 60 17 BspEI T'CCGG_A Cuts at: 2785 5451 2785 Size: 2666 3122 BspHI T'CATG_A Cuts at: 2749 3677 5407 2749 Size: 928 1730 3130 Fragments arranged by size: 3130 1730 928 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2304 2438 3143 844 Size: 1460 134 705 3489 Fragments arranged by size: 3489 1460 705 134 BsrBI CCG'CTC Cuts at: 1394 1823 3675 1394 Size: 429 1852 3507 Fragments arranged by size: 3507 1852 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4283 4457 65 Size: 1183 3035 174 1396 Fragments arranged by size: 3035 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4432 4619 1700 Size: 110 2622 187 2869 Fragments arranged by size: 2869 2622 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5788 BssSI C'ACGA_G Cuts at: 3848 4860 3848 Size: 1012 4776 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5788 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstXI CCAn_nnnn'nTGG Cuts at: 2272 2798 2272 Size: 526 5262 BstYI r'GATC_y Cuts at: 1972 3080 3118 3485 3870 3887 5339 5425 5437 Size: 1108 38 367 385 17 1452 86 12 Cuts at: 5437 5783 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1108 385 367 346 86 38 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5236 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 3304 4098 4118 3304 Size: 794 20 4974 Fragments arranged by size: 4974 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 3092 3092 Size: 5788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4795 809 Size: 507 3479 1802 Fragments arranged by size: 3479 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2289 2590 3235 Size: 54 1425 50 275 18 459 301 645 Cuts at: 3235 4124 5753 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 645 459 301 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3235 1537 Size: 275 1423 4090 Fragments arranged by size: 4090 1423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2817 3053 3718 1353 Size: 1464 236 665 3423 Fragments arranged by size: 3423 1464 665 236 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4375 4749 4895 387 Size: 1064 138 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2060 2060 Size: 5788 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2272 2528 2996 3851 4448 5235 Size: 393 458 182 256 468 855 597 787 Cuts at: 5235 5503 1239 Size: 268 1524 Fragments arranged by size: 1524 855 787 597 468 458 393 268 256 182 EcoO109I rG'GnC_Cy Cuts at: 2530 2530 Size: 5788 EcoRI G'AATT_C Cuts at: 3097 3475 3097 Size: 378 5410 FspI TGC'GCA Cuts at: 4294 4294 Size: 5788 HaeII r_GCGC'y Cuts at: 1678 2251 4935 1678 Size: 573 2684 2531 Fragments arranged by size: 2684 2531 573 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4429 4461 4503 4535 1373 Size: 32 135 32 2857 32 42 32 2626 Fragments arranged by size: 2857 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3132 3378 669 Size: 988 281 1194 246 3079 Fragments arranged by size: 3079 1194 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KpnI G_GTAC'C Cuts at: 3113 3113 Size: 5788 MfeI C'AATT_G Cuts at: 3387 3387 Size: 5788 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4902 5086 1305 Size: 3597 184 2007 Fragments arranged by size: 3597 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2592 10 Size: 54 1425 343 760 3206 Fragments arranged by size: 3206 1425 760 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2438 2697 3746 4105 4264 518 Size: 648 1272 259 1049 359 159 2042 Fragments arranged by size: 2042 1272 1049 648 359 259 159 MspA1I CmG'CkG Cuts at: 1606 2548 2953 3050 3204 3881 5029 5274 1606 Size: 942 405 97 154 677 1148 245 2120 Fragments arranged by size: 2120 1148 942 677 405 245 154 97 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5236 NdeI CA'TA_TG Cuts at: 387 387 Size: 5788 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 2504 3146 4691 1132 Size: 97 1275 642 1545 2229 Fragments arranged by size: 2229 1545 1275 642 97 PasI CC'CwG_GG Cuts at: 1348 3068 1348 Size: 1720 4068 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 2210 2250 1108 Size: 1102 40 4646 Fragments arranged by size: 4646 1102 40 PfoI T'CCnGG_A Cuts at: 2067 2067 Size: 5788 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2112 2144 3857 3889 5370 5402 2112 Size: 32 1713 32 1481 32 2498 Fragments arranged by size: 2498 1713 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PspOMI G'GGCC_C Cuts at: 2529 2529 Size: 5788 PstI C_TGCA'G Cuts at: 830 3140 830 Size: 2310 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 PvuII CAG'CTG Cuts at: 2548 2953 3050 2548 Size: 405 97 5286 Fragments arranged by size: 5286 405 97 SacI G_AGCT'C Cuts at: 2062 2062 Size: 5788 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SbfI CC_TGCA'GG Cuts at: 3140 3140 Size: 5788 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 2176 3136 4271 4952 5143 826 Size: 219 1131 960 1135 681 191 1471 Fragments arranged by size: 1471 1135 1131 960 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5788 SmaI CCC'GGG Cuts at: 1872 2084 3115 1872 Size: 212 1031 4545 Fragments arranged by size: 4545 1031 212 SmlI C'TyrA_G Cuts at: 820 1017 1943 3892 4793 5055 820 Size: 197 926 1949 901 262 1553 Fragments arranged by size: 1949 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 2504 3146 1132 Size: 1372 642 3774 Fragments arranged by size: 3774 1372 642 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 2933 1517 Size: 1416 4372 StyI C'CwwG_G Cuts at: 513 1065 2194 2452 2686 3227 513 Size: 552 1129 258 234 541 3074 Fragments arranged by size: 3074 1129 552 541 258 234 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2481 3026 3815 3832 3985 4170 4615 Size: 1255 901 545 789 17 153 185 445 Cuts at: 4615 325 Size: 1498 Fragments arranged by size: 1498 1255 901 789 545 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 2971 4034 Size: 274 80 33 51 1443 171 823 1063 Cuts at: 4034 5746 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1063 823 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2038 2070 5748 5780 2038 Size: 32 3678 32 2046 Fragments arranged by size: 3678 2046 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2665 1316 Size: 1349 4439 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2269 2530 2269 Size: 261 5527 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 2082 3113 1870 Size: 212 1031 4545 Fragments arranged by size: 4545 1031 212 XmnI GAAnn'nnTTC Cuts at: 3917 3917 Size: 5788 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3596 276 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI