(Circular) (Six-base) MAPSORT of: pFN21AE0805.seq Check: 4848 from: 1 to: 5785 pFN21AE0805 5785 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2800 2924 2800 Size: 124 5661 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3595 278 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2535 2658 3128 1937 Size: 598 123 470 4594 Fragments arranged by size: 4594 598 470 123 Acc65I G'GTAC_C Cuts at: 3106 3106 Size: 5785 AclI AA'CG_TT Cuts at: 1563 3912 4285 1563 Size: 2349 373 3063 Fragments arranged by size: 3063 2349 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2176 3848 5232 5500 1632 Size: 544 1672 1384 268 1917 Fragments arranged by size: 1917 1672 1384 544 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5588 AflIII A'CryG_T Cuts at: 1203 2742 4684 5661 1203 Size: 1539 1942 977 1327 Fragments arranged by size: 1942 1539 1327 977 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 4514 4514 Size: 5785 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AlwNI CAG_nnn'CTG Cuts at: 3528 5100 3528 Size: 1572 4213 ApaI G_GGCC'C Cuts at: 2884 2884 Size: 5785 ApaLI G'TGCA_C Cuts at: 2738 3842 4998 2738 Size: 1104 1156 3525 Fragments arranged by size: 3525 1156 1104 ApoI r'AATT_y Cuts at: 1493 3094 3320 3472 5581 1493 Size: 1601 226 152 2109 1697 Fragments arranged by size: 2109 1697 1601 226 152 AseI AT'TA_AT Cuts at: 160 1032 4339 160 Size: 872 3307 1606 Fragments arranged by size: 3307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 2240 3110 1870 Size: 73 297 870 4545 Fragments arranged by size: 4545 870 297 73 BamHI G'GATC_C Cuts at: 3115 3482 3115 Size: 367 5418 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2726 3106 4561 618 Size: 325 217 627 939 380 1455 1842 Fragments arranged by size: 1842 1455 939 627 380 325 217 BanII G_rGCy'C Cuts at: 1391 2088 2884 3104 1391 Size: 697 796 220 4072 Fragments arranged by size: 4072 796 697 220 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2221 928 Size: 971 322 4492 Fragments arranged by size: 4492 971 322 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3976 4010 1903 Size: 34 2039 34 3678 Fragments arranged by size: 3678 2039 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3677 4887 3677 Size: 1210 4575 BclI T'GATC_A Cuts at: 1548 1548 Size: 5785 BfrBI ATG'CAT Cuts at: 2826 2826 Size: 5785 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4396 136 Size: 107 122 71 631 605 2724 1525 Fragments arranged by size: 2724 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5780 5780 Size: 5785 BlpI GC'TnA_GC Cuts at: 3202 3202 Size: 5785 Bme1580I G_kGCm'C Cuts at: 1790 2729 2742 2884 3846 5002 1790 Size: 939 13 142 962 1156 2573 Fragments arranged by size: 2573 1156 962 939 142 13 BmgBI CAC'GTC Cuts at: 1318 2767 1318 Size: 1449 4336 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3516 4474 442 Size: 416 53 83 399 24 2099 958 1753 Fragments arranged by size: 2099 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 2102 2414 4445 1239 Size: 809 54 312 2031 2579 Fragments arranged by size: 2579 2031 809 312 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3910 4775 5073 3910 Size: 865 298 4622 Fragments arranged by size: 4622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2390 4448 882 Size: 617 891 2058 2219 Fragments arranged by size: 2219 2058 891 617 BsaAI yAC'GTr Cuts at: 493 1204 2737 3576 493 Size: 711 1533 839 2702 Fragments arranged by size: 2702 1533 839 711 BsaBI GATnn'nnATC Cuts at: 2411 2489 5779 2411 Size: 78 3290 2417 Fragments arranged by size: 3290 2417 78 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2634 3592 3974 4609 Size: 53 83 186 936 1101 958 382 635 Cuts at: 4609 275 Size: 1451 Fragments arranged by size: 1451 1101 958 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3018 4218 4616 4890 5037 5448 3018 Size: 1200 398 274 147 411 3355 Fragments arranged by size: 3355 1200 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2025 2055 2309 2339 1178 Size: 30 165 30 622 30 254 30 4624 Fragments arranged by size: 4624 622 254 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2708 1186 Size: 1522 4263 BseYI C'CCAG_C Cuts at: 2088 2877 4988 5484 2088 Size: 789 2111 496 2389 Fragments arranged by size: 2389 2111 789 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2306 2747 1163 Size: 573 570 441 4201 Fragments arranged by size: 4201 573 570 441 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3235 3996 5024 1540 Size: 275 186 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2175 2742 3846 3931 5002 2175 Size: 567 1104 85 1071 2958 Fragments arranged by size: 2958 1104 1071 567 85 BsmI GAATG_Cn' Cuts at: 2125 3296 3389 2125 Size: 1171 93 4521 Fragments arranged by size: 4521 1171 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2175 2729 2742 2884 3104 3846 Size: 399 298 87 554 13 142 220 742 Cuts at: 3846 3931 5002 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 554 399 298 220 142 87 85 13 BspEI T'CCGG_A Cuts at: 3018 5448 3018 Size: 2430 3355 BspHI T'CATG_A Cuts at: 2403 2580 3674 5404 2403 Size: 177 1094 1730 2784 Fragments arranged by size: 2784 1730 1094 177 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2215 2328 2800 2924 3140 844 Size: 1371 113 472 124 216 3489 Fragments arranged by size: 3489 1371 472 216 124 113 BsrBI CCG'CTC Cuts at: 1394 1823 3672 1394 Size: 429 1849 3507 Fragments arranged by size: 3507 1849 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2121 4280 4454 65 Size: 1183 873 2159 174 1396 Fragments arranged by size: 2159 1396 1183 873 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4429 4616 1700 Size: 110 2619 187 2869 Fragments arranged by size: 2869 2619 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 3845 4857 3845 Size: 1012 4773 BstAPI GCAn_nnn'nTGC Cuts at: 794 2120 794 Size: 1326 4459 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5785 BstXI CCAn_nnnn'nTGG Cuts at: 2392 2857 2392 Size: 465 5320 BstYI r'GATC_y Cuts at: 1972 3115 3482 3867 3884 5336 5422 5434 5780 Size: 1143 367 385 17 1452 86 12 346 Cuts at: 5780 1972 Size: 1977 Fragments arranged by size: 1977 1452 1143 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2301 513 Size: 552 1236 3997 Fragments arranged by size: 3997 1236 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2512 487 Size: 683 56 1286 3760 Fragments arranged by size: 3760 1286 683 56 BtsI GCAGTG_nn' Cuts at: 2153 2783 2946 3301 4095 4115 2153 Size: 630 163 355 794 20 3823 Fragments arranged by size: 3823 794 630 355 163 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 3089 3089 Size: 5785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2543 4792 809 Size: 507 1227 2249 1802 Fragments arranged by size: 2249 1802 1227 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2298 2361 2523 Size: 54 1425 50 275 18 468 63 162 Cuts at: 2523 2613 3232 4121 5750 8 Size: 90 619 889 1629 43 Fragments arranged by size: 1629 1425 889 619 468 275 162 90 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3232 1537 Size: 275 1420 4090 Fragments arranged by size: 4090 1420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3715 1353 Size: 2362 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4372 4746 4892 387 Size: 1064 138 2783 374 146 1280 Fragments arranged by size: 2783 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2102 2176 2414 3848 4445 5232 Size: 393 416 54 74 238 1434 597 787 Cuts at: 5232 5500 1239 Size: 268 1524 Fragments arranged by size: 1524 1434 787 597 416 393 268 238 74 54 EcoO109I rG'GnC_Cy Cuts at: 2596 2881 2596 Size: 285 5500 EcoRI G'AATT_C Cuts at: 3094 3472 3094 Size: 378 5407 FspI TGC'GCA Cuts at: 3035 4291 3035 Size: 1256 4529 HaeII r_GCGC'y Cuts at: 1678 2214 4932 1678 Size: 536 2718 2531 Fragments arranged by size: 2718 2531 536 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2025 2057 4426 4458 4500 Size: 32 135 32 453 32 2369 32 42 Cuts at: 4500 4532 1373 Size: 32 2626 Fragments arranged by size: 2626 2369 453 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2536 3129 3375 669 Size: 988 281 598 593 246 3079 Fragments arranged by size: 3079 988 598 593 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5785 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KpnI G_GTAC'C Cuts at: 3110 3110 Size: 5785 MfeI C'AATT_G Cuts at: 3384 3384 Size: 5785 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4899 5083 1305 Size: 3594 184 2007 Fragments arranged by size: 3594 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2525 2615 10 Size: 54 1425 343 693 90 3180 Fragments arranged by size: 3180 1425 693 343 90 54 MslI CAynn'nnrTG Cuts at: 518 1166 2585 2696 3743 4102 4261 518 Size: 648 1419 111 1047 359 159 2042 Fragments arranged by size: 2042 1419 1047 648 359 159 111 MspA1I CmG'CkG Cuts at: 1606 3201 3878 5026 5271 1606 Size: 1595 677 1148 245 2120 Fragments arranged by size: 2120 1595 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5233 NdeI CA'TA_TG Cuts at: 387 387 Size: 5785 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NsiI A_TGCA'T Cuts at: 2828 2828 Size: 5785 NspI r_CATG'y Cuts at: 1132 1229 2371 2746 3143 4688 1132 Size: 97 1142 375 397 1545 2229 Fragments arranged by size: 2229 1545 1142 397 375 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5785 PciI A'CATG_T Cuts at: 2742 4684 2742 Size: 1942 3843 PflMI CCAn_nnn'nTGG Cuts at: 1108 2461 1108 Size: 1353 4432 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5785 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PspOMI G'GGCC_C Cuts at: 2880 2880 Size: 5785 PstI C_TGCA'G Cuts at: 830 2351 3137 830 Size: 1521 786 3478 Fragments arranged by size: 3478 1521 786 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 SalI G'TCGA_C Cuts at: 1936 2534 3127 1936 Size: 598 593 4594 Fragments arranged by size: 4594 598 593 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 4033 5745 4033 Size: 1712 4073 SexAI A'CCwGG_T Cuts at: 2898 2967 2898 Size: 69 5716 SfcI C'TryA_G Cuts at: 826 1045 2347 3133 4268 4949 5140 826 Size: 219 1302 786 1135 681 191 1471 Fragments arranged by size: 1471 1302 1135 786 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3889 4790 5052 820 Size: 197 926 1946 901 262 1553 Fragments arranged by size: 1946 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5785 StyI C'CwwG_G Cuts at: 513 1065 3224 513 Size: 552 2159 3074 Fragments arranged by size: 3074 2159 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3812 3829 3982 4167 4612 325 Size: 1255 2232 17 153 185 445 1498 Fragments arranged by size: 2232 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2334 4031 5743 Size: 274 80 33 51 1443 357 1697 1712 Cuts at: 5743 96 Size: 138 Fragments arranged by size: 1712 1697 1443 357 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5745 5777 5745 Size: 32 5753 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5785 XbaI T'CTAG_A Cuts at: 3121 3121 Size: 5785 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5785 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 3914 3914 Size: 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3593 276 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI