(Circular) (Six-base) MAPSORT of: pFN21AE0804.seq Check: 487 from: 1 to: 5572 pFN21AE0804 5572 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3382 278 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2915 1937 Size: 978 4594 Acc65I G'GTAC_C Cuts at: 2893 2893 Size: 5572 AclI AA'CG_TT Cuts at: 1563 3699 4072 1563 Size: 2136 373 3063 Fragments arranged by size: 3063 2136 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2284 3635 5019 5287 1632 Size: 652 1351 1384 268 1917 Fragments arranged by size: 1917 1384 1351 652 268 AfeI AGC'GCT Cuts at: 2312 2312 Size: 5572 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5375 AflIII A'CryG_T Cuts at: 1203 4471 5448 1203 Size: 3268 977 1327 Fragments arranged by size: 3268 1327 977 AgeI A'CCGG_T Cuts at: 2083 4403 2083 Size: 2320 3252 AhdI GACnn_n'nnGTC Cuts at: 4301 4301 Size: 5572 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5572 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5572 AlwNI CAG_nnn'CTG Cuts at: 2671 2854 3315 4887 2671 Size: 183 461 1572 3356 Fragments arranged by size: 3356 1572 461 183 ApaI G_GGCC'C Cuts at: 2138 2815 2138 Size: 677 4895 ApaLI G'TGCA_C Cuts at: 3629 4785 3629 Size: 1156 4416 ApoI r'AATT_y Cuts at: 1493 2881 3107 3259 5368 1493 Size: 1388 226 152 2109 1697 Fragments arranged by size: 2109 1697 1388 226 152 AseI AT'TA_AT Cuts at: 160 1032 4126 160 Size: 872 3094 1606 Fragments arranged by size: 3094 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 AvaI C'yCGr_G Cuts at: 1870 1943 2075 2755 2774 2897 1870 Size: 73 132 680 19 123 4545 Fragments arranged by size: 4545 680 132 123 73 19 BamHI G'GATC_C Cuts at: 2902 3269 2902 Size: 367 5205 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2228 2825 2837 2893 4348 Size: 325 217 627 441 597 12 56 1455 Cuts at: 4348 618 Size: 1842 Fragments arranged by size: 1842 1455 627 597 441 325 217 56 12 BanII G_rGCy'C Cuts at: 1391 2138 2283 2815 2891 1391 Size: 747 145 532 76 4072 Fragments arranged by size: 4072 747 532 145 76 BbeI G_GCGC'C Cuts at: 2232 2829 2232 Size: 597 4975 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2041 2641 928 Size: 971 142 600 3859 Fragments arranged by size: 3859 971 600 142 BbvCI CC'TCA_GC Cuts at: 2792 2792 Size: 5572 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2819 2853 3763 3797 1903 Size: 34 882 34 910 34 3678 Fragments arranged by size: 3678 910 882 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3464 4674 3464 Size: 1210 4362 BclI T'GATC_A Cuts at: 1548 1548 Size: 5572 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2199 2531 4183 Size: 107 122 71 631 605 527 332 1652 Cuts at: 4183 136 Size: 1525 Fragments arranged by size: 1652 1525 631 605 527 332 122 107 71 BglII A'GATC_T Cuts at: 5567 5567 Size: 5572 BlpI GC'TnA_GC Cuts at: 2989 2989 Size: 5572 Bme1580I G_kGCm'C Cuts at: 1790 2138 2462 2815 3633 4789 1790 Size: 348 324 353 818 1156 2573 Fragments arranged by size: 2573 1156 818 353 348 324 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5572 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3303 4261 442 Size: 416 53 83 399 24 1886 958 1753 Fragments arranged by size: 1886 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5572 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2047 2677 4232 1239 Size: 808 630 1555 2579 Fragments arranged by size: 2579 1555 808 630 Bpu10I CC'TnA_GC Cuts at: 2214 2792 2214 Size: 578 4994 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3697 4562 4860 3697 Size: 865 298 4409 Fragments arranged by size: 4409 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2870 4235 882 Size: 617 1371 1365 2219 Fragments arranged by size: 2219 1371 1365 617 BsaAI yAC'GTr Cuts at: 493 1204 2485 3363 493 Size: 711 1281 878 2702 Fragments arranged by size: 2702 1281 878 711 BsaBI GATnn'nnATC Cuts at: 5566 5566 Size: 5572 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2229 2826 3379 3761 Size: 53 83 186 936 696 597 553 382 Cuts at: 3761 4396 275 Size: 635 1451 Fragments arranged by size: 1451 936 696 635 597 553 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2083 4005 4403 4677 4824 5235 2083 Size: 1922 398 274 147 411 2420 Fragments arranged by size: 2420 1922 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2720 2750 1178 Size: 30 165 30 1317 30 4000 Fragments arranged by size: 4000 1317 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5572 BseYI C'CCAG_C Cuts at: 2833 4775 5271 2833 Size: 1942 496 3134 Fragments arranged by size: 3134 1942 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2141 1163 Size: 573 405 4594 Fragments arranged by size: 4594 573 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2373 2412 3022 3783 4811 1540 Size: 275 186 372 39 610 761 1028 2301 Fragments arranged by size: 2301 1028 761 610 372 275 186 39 BsiHKAI G_wGCw'C Cuts at: 2106 2256 3633 3718 4789 2106 Size: 150 1377 85 1071 2889 Fragments arranged by size: 2889 1377 1071 150 85 BsmI GAATG_Cn' Cuts at: 3083 3176 3083 Size: 93 5479 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2138 2256 2283 2462 2815 2891 Size: 399 316 32 118 27 179 353 76 Cuts at: 2891 3633 3718 4789 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 353 316 179 118 85 76 32 27 BspEI T'CCGG_A Cuts at: 5235 5235 Size: 5572 BspHI T'CATG_A Cuts at: 3461 5191 3461 Size: 1730 3842 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2160 2570 2927 844 Size: 1316 410 357 3489 Fragments arranged by size: 3489 1316 410 357 BsrBI CCG'CTC Cuts at: 1394 1823 3459 1394 Size: 429 1636 3507 Fragments arranged by size: 3507 1636 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4067 4241 65 Size: 1183 2819 174 1396 Fragments arranged by size: 2819 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2083 2352 2407 4216 4403 1700 Size: 110 273 269 55 1809 187 2869 Fragments arranged by size: 2869 1809 273 269 187 110 55 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5572 BssHII G'CGCG_C Cuts at: 1925 2610 1925 Size: 685 4887 BssSI C'ACGA_G Cuts at: 3632 4644 3632 Size: 1012 4560 BstAPI GCAn_nnn'nTGC Cuts at: 794 2671 794 Size: 1877 3695 BstBI TT'CG_AA Cuts at: 4376 4376 Size: 5572 BstEII G'GTnAC_C Cuts at: 1184 2557 1184 Size: 1373 4199 BstXI CCAn_nnnn'nTGG Cuts at: 2716 2716 Size: 5572 BstYI r'GATC_y Cuts at: 1972 2902 3269 3654 3671 5123 5209 5221 5567 Size: 930 367 385 17 1452 86 12 346 Cuts at: 5567 1972 Size: 1977 Fragments arranged by size: 1977 1452 930 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2208 2579 2859 513 Size: 552 1143 371 280 3226 Fragments arranged by size: 3226 1143 552 371 280 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4833 Fragments arranged by size: 4833 683 56 BtsI GCAGTG_nn' Cuts at: 3088 3882 3902 3088 Size: 794 20 4758 Fragments arranged by size: 4758 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5572 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5537 DraI TTT'AAA Cuts at: 2876 2876 Size: 5572 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2045 4579 809 Size: 507 729 2534 1802 Fragments arranged by size: 2534 1802 729 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2163 2370 2409 Size: 54 1425 50 275 18 333 207 39 Cuts at: 2409 2487 2706 3019 3908 5537 8 Size: 78 219 313 889 1629 43 Fragments arranged by size: 1629 1425 889 333 313 275 219 207 78 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2370 2409 3019 1537 Size: 275 558 39 610 4090 Fragments arranged by size: 4090 610 558 275 39 EarI CTCTTCn'nnn_ Cuts at: 1353 2333 2630 3502 1353 Size: 980 297 872 3423 Fragments arranged by size: 3423 980 872 297 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4159 4533 4679 387 Size: 1064 138 2570 374 146 1280 Fragments arranged by size: 2570 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2047 2284 2677 3635 4232 5019 5287 Size: 393 415 237 393 958 597 787 268 Cuts at: 5287 1239 Size: 1524 Fragments arranged by size: 1524 958 787 597 415 393 393 268 237 EcoNI CCTnn'n_nnAGG Cuts at: 2819 2819 Size: 5572 EcoO109I rG'GnC_Cy Cuts at: 2134 2135 2811 2812 2134 Size: 1 676 1 4894 Fragments arranged by size: 4894 676 1 1 EcoRI G'AATT_C Cuts at: 2881 3259 2881 Size: 378 5194 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2155 2155 Size: 5572 FspI TGC'GCA Cuts at: 2447 4078 2447 Size: 1631 3941 HaeII r_GCGC'y Cuts at: 1678 2232 2314 2829 4719 1678 Size: 554 82 515 1890 2531 Fragments arranged by size: 2531 1890 554 515 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4213 4245 4287 4319 1373 Size: 32 135 32 2641 32 42 32 2626 Fragments arranged by size: 2641 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2728 2916 3162 669 Size: 988 281 790 188 246 3079 Fragments arranged by size: 3079 988 790 281 246 188 HindIII A'AGCT_T Cuts at: 748 748 Size: 5572 HpaI GTT'AAC Cuts at: 3162 3162 Size: 5572 KasI G'GCGC_C Cuts at: 2228 2825 2228 Size: 597 4975 KpnI G_GTAC'C Cuts at: 2897 2897 Size: 5572 MfeI C'AATT_G Cuts at: 3171 3171 Size: 5572 MluI A'CGCG_T Cuts at: 5448 5448 Size: 5572 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2346 4686 4870 1305 Size: 1041 2340 184 2007 Fragments arranged by size: 2340 2007 1041 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2489 2708 10 Size: 54 1425 343 657 219 2874 Fragments arranged by size: 2874 1425 657 343 219 54 MslI CAynn'nnrTG Cuts at: 518 1166 3530 3889 4048 518 Size: 648 2364 359 159 2042 Fragments arranged by size: 2364 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2419 2581 2704 2791 2988 3665 4813 5058 Size: 813 162 123 87 197 677 1148 245 Cuts at: 5058 1606 Size: 2120 Fragments arranged by size: 2120 1148 813 677 245 197 162 123 87 NaeI GCC'GGC Cuts at: 2354 2409 2354 Size: 55 5517 NarI GG'CG_CC Cuts at: 2229 2826 2229 Size: 597 4975 NcoI C'CATG_G Cuts at: 513 1065 2859 513 Size: 552 1794 3226 Fragments arranged by size: 3226 1794 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5572 NgoMIV G'CCGG_C Cuts at: 2352 2407 2352 Size: 55 5517 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5572 NotI GC'GGCC_GC Cuts at: 3019 3019 Size: 5572 NspI r_CATG'y Cuts at: 1132 1229 2930 4475 1132 Size: 97 1701 1545 2229 Fragments arranged by size: 2229 1701 1545 97 PasI CC'CwG_GG Cuts at: 1348 2224 2815 1348 Size: 876 591 4105 Fragments arranged by size: 4105 876 591 PciI A'CATG_T Cuts at: 4471 4471 Size: 5572 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5572 PmeI GTTT'AAAC Cuts at: 2876 2876 Size: 5572 PmlI CAC'GTG Cuts at: 2485 2485 Size: 5572 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3641 3673 5154 5186 3641 Size: 32 1481 32 4027 Fragments arranged by size: 4027 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5572 PsiI TTA'TAA Cuts at: 3142 3142 Size: 5572 PspOMI G'GGCC_C Cuts at: 2134 2811 2134 Size: 677 4895 PstI C_TGCA'G Cuts at: 830 2667 2924 830 Size: 1837 257 3478 Fragments arranged by size: 3478 1837 257 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5572 SacII CC_GC'GG Cuts at: 2582 2582 Size: 5572 SalI G'TCGA_C Cuts at: 1936 2914 1936 Size: 978 4594 SapI GCTCTTCn'nnn_ Cuts at: 2630 2630 Size: 5572 SbfI CC_TGCA'GG Cuts at: 2924 2924 Size: 5572 ScaI AGT'ACT Cuts at: 3820 5532 3820 Size: 1712 3860 SexAI A'CCwGG_T Cuts at: 2498 2498 Size: 5572 SfcI C'TryA_G Cuts at: 826 1045 2663 2920 4055 4736 4927 826 Size: 219 1618 257 1135 681 191 1471 Fragments arranged by size: 1618 1471 1135 681 257 219 191 SfoI GGC'GCC Cuts at: 2230 2827 2230 Size: 597 4975 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5572 SmaI CCC'GGG Cuts at: 1872 2776 2899 1872 Size: 904 123 4545 Fragments arranged by size: 4545 904 123 SmlI C'TyrA_G Cuts at: 820 1017 1943 3676 4577 4839 820 Size: 197 926 1733 901 262 1553 Fragments arranged by size: 1733 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3363 493 Size: 2870 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5572 SphI G_CATG'C Cuts at: 1132 2930 1132 Size: 1798 3774 SspI AAT'ATT Cuts at: 5 52 3496 5 Size: 47 3444 2081 Fragments arranged by size: 3444 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5572 StyI C'CwwG_G Cuts at: 513 1065 2859 3011 513 Size: 552 1794 152 3074 Fragments arranged by size: 3074 1794 552 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2545 2635 2825 3599 3616 3769 3954 Size: 1255 965 90 190 774 17 153 185 Cuts at: 3954 4399 325 Size: 445 1498 Fragments arranged by size: 1498 1255 965 774 445 190 185 153 90 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3818 5530 96 Size: 274 80 33 51 1443 1841 1712 138 Fragments arranged by size: 1841 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5532 5564 5532 Size: 32 5540 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5572 XbaI T'CTAG_A Cuts at: 2908 2908 Size: 5572 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5572 XmaI C'CCGG_G Cuts at: 1870 2774 2897 1870 Size: 904 123 4545 Fragments arranged by size: 4545 904 123 XmnI GAAnn'nnTTC Cuts at: 3701 3701 Size: 5572 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3380 276 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SfiI SrfI SwaI XcmI