Restriction map for
pFN21AE0802
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoT22I FauI FbaI FseI FspI
HaeII HapII HhaI HinP1I HpaII
HphI KasI KpnI KspI MaeI
MfeI MluI MroI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tsp45I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 877 GTCTAC GT^[AC][GT]AC
AcsI 5 AAATTT [GA]^AATT[CT]
AcyI 501 GGCGTC G[GA]^CG[CT]C
Alw44I 787 GTGCAC G^TGCAC
AlwI 900 GATCC GGATC(4/5)
ApaLI 787 GTGCAC G^TGCAC
ApoI 5 AAATTT [GA]^AATT[CT]
AvaII 370 GGACC G^G[AT]CC
BanI 907 GGTGCC G^G[CT][GA]CC
BanII 661 GGGCTC G[GA]GC[CT]^C
BsaHI 501 GGCGTC G[GA]^CG[CT]C
BsgI 548 CTGCAC GTGCAG(16/14)
BsmAI 226 GTCTC GTCTC(1/5)
BsmI 920 GAATGC GAATGC(1/-1)
BspLU11I 724 ACATGT A^CATGT
BstXI 360 CCATGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 899 AGATCC [GA]^GATC[CT]
Cfr13I 370 GGACC G^G[ACGT]CC
DdeI 398 CTGAG C^T[ACGT]AG
DsaI 903 CCATGG C^C[GA][CT]GG
EcoRV 87 GATATC GAT^ATC
Hin1I 501 GGCGTC G[GA]^CG[CT]C
HincII 586 GTTAAC GT[CT]^[GA]AC
HindII 586 GTTAAC GT[CT]^[GA]AC
HindIII 293 AAGCTT A^AGCTT
HpaI 586 GTTAAC GTT^AAC
MaeIII 613 GTTAC ^GT[ACGT]AC
MflI 899 AGATCC [GA]^GATC[CT]
MslI 736 CACGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 345 CAGCTG C[AC]G^C[GT]G
NcoI 903 CCATGG C^CATGG
NspI 724 ACATGT [GA]CATG^[CT]
PleI 70 GAGTC GAGTC(4/5)
PvuII 345 CAGCTG CAG^CTG
Sau96I 370 GGACC G^G[ACGT]CC
TfiI 634 GATTC G^A[AT]TC
XcmI 200 CCATGTTGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 899 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AciI 373 CCGC CCGC(-3/-1)
792 CCGC
AfaI 186 GTAC GT^AC
455 GTAC
AflIII 724 ACATGT A^C[GA][CT]GT
735 ACACGT
AspHI 76 GAGCAC G[AT]GC[AT]^C
787 GTGCAC
BbvI 352 GCTGC GCAGC(8/12)
819 GCTGC
BglI 199 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
826 GCCATGATGGC
BpmI 67 CTGGAG CTGGAG(16/14)
695 CTGGAG
BsaBI 108 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
645 GATTGTGATC
BsiHKAI 76 GAGCAC G[AT]GC[AT]^C
787 GTGCAC
BsmFI 94 GTCCC GGGAC(10/14)
810 GTCCC
BsrI 210 ACTGG ACTGG(1/-1)
694 ACTGG
BssKI 61 CCAGG ^CC[ACGT]GG
426 CCAGG
BstNI 61 CCAGG CC^[AT]GG
426 CCAGG
DpnII 651 GATC ^GATC
900 GATC
EarI 190 CTCTTC CTCTTC(1/4)
847 CTCTTC
EcoRII 61 CCAGG ^CC[AT]GG
426 CCAGG
Fnu4HI 352 GCTGC GC^[ACGT]GC
819 GCTGC
HgaI 938 GACGC GACGC(5/10)
502 GCGTC
HinfI 70 GAGTC G^A[ACGT]TC
634 GATTC
ItaI 352 GCTGC GC^[ACGT]GC
819 GCTGC
Ksp632I 190 CTCTTC CTCTTC(1/4)
847 CTCTTC
MaeII 149 ACGT A^CGT
737 ACGT
MamI 108 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
645 GATTGTGATC
MboI 651 GATC ^GATC
900 GATC
MseI 587 TTAA T^TAA
887 TTAA
MvaI 61 CCAGG CC^[AT]GG
426 CCAGG
NdeII 651 GATC ^GATC
900 GATC
RsaI 186 GTAC GT^AC
455 GTAC
Sau3AI 651 GATC ^GATC
900 GATC
ScrFI 61 CCAGG CC^[ACGT]GG
426 CCAGG
Tru9I 587 TTAA T^TAA
887 TTAA
TseI 352 GCTGC G^C[AT]GC
819 GCTGC
Tsp509I 6 AATT ^AATT
282 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI BalI BmyI BsaJI BsiYI
BslI Bsp1286I BstF5I Cac8I EaeI
EcoT14I FokI HaeIII MboII MluNI
MnlI MscI MwoI NlaIII NlaIV
SfaNI SfcI StyI TspRI