(Circular) (Six-base) MAPSORT of: pFN21AE0802.seq Check: 7329 from: 1 to: 5653 pFN21AE0802 5653 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2883 2996 1937 Size: 946 113 4594 Fragments arranged by size: 4594 946 113 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3716 5100 5368 1632 Size: 2084 1384 268 1917 Fragments arranged by size: 2084 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 2729 2740 4552 5529 1203 Size: 1526 11 1812 977 1327 Fragments arranged by size: 1812 1526 1327 977 11 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 2745 1166 Size: 1579 4074 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5653 AlwNI CAG_nnn'CTG Cuts at: 3396 4968 3396 Size: 1572 4081 ApaLI G'TGCA_C Cuts at: 2792 3710 4866 2792 Size: 918 1156 3579 Fragments arranged by size: 3579 1156 918 ApoI r'AATT_y Cuts at: 1493 2010 2962 3188 3340 5449 1493 Size: 517 952 226 152 2109 1697 Fragments arranged by size: 2109 1697 952 517 226 152 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2978 1870 Size: 73 1035 4545 Fragments arranged by size: 4545 1035 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2174 2207 2174 Size: 33 5620 BamHI G'GATC_C Cuts at: 2983 3350 2983 Size: 367 5286 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2912 2974 4429 618 Size: 325 217 627 1125 62 1455 1842 Fragments arranged by size: 1842 1455 1125 627 325 217 62 BanII G_rGCy'C Cuts at: 1391 2670 2972 1391 Size: 1279 302 4072 Fragments arranged by size: 4072 1279 302 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4682 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3844 3878 1903 Size: 34 1907 34 3678 Fragments arranged by size: 3678 1907 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 1548 Size: 5653 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2210 2837 4264 Size: 107 122 71 631 605 538 627 1427 Cuts at: 4264 136 Size: 1525 Fragments arranged by size: 1525 1427 631 627 605 538 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 3070 3070 Size: 5653 Bme1580I G_kGCm'C Cuts at: 1790 2796 3714 4870 1790 Size: 1006 918 1156 2573 Fragments arranged by size: 2573 1156 1006 918 BmgBI CAC'GTC Cuts at: 1318 2155 2743 1318 Size: 837 588 4228 Fragments arranged by size: 4228 837 588 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3384 4342 442 Size: 416 53 83 399 24 1967 958 1753 Fragments arranged by size: 1967 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2721 4313 1239 Size: 854 628 1592 2579 Fragments arranged by size: 2579 1592 854 628 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3778 4643 4941 3778 Size: 865 298 4490 Fragments arranged by size: 4490 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4316 882 Size: 617 2817 2219 Fragments arranged by size: 2817 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3444 493 Size: 711 2240 2702 Fragments arranged by size: 2702 2240 711 BsaBI GATnn'nnATC Cuts at: 2117 2654 5647 2117 Size: 537 2993 2123 Fragments arranged by size: 2993 2123 537 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2507 3460 3842 4477 Size: 53 83 186 936 974 953 382 635 Cuts at: 4477 275 Size: 1451 Fragments arranged by size: 1451 974 953 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4086 4484 4758 4905 5316 4086 Size: 398 274 147 411 4423 Fragments arranged by size: 4423 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5428 Fragments arranged by size: 5428 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5653 BseYI C'CCAG_C Cuts at: 4856 5352 4856 Size: 496 5157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2538 1163 Size: 573 802 4278 Fragments arranged by size: 4278 802 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 3864 4892 1540 Size: 275 186 1102 761 1028 2301 Fragments arranged by size: 2301 1102 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2085 2796 3714 3799 4870 2085 Size: 711 918 85 1071 2868 Fragments arranged by size: 2868 1071 918 711 85 BsmI GAATG_Cn' Cuts at: 2931 3164 3257 2931 Size: 233 93 5327 Fragments arranged by size: 5327 233 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2670 2796 2972 3714 3799 4870 Size: 399 295 585 126 176 742 85 1071 Cuts at: 4870 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 585 399 295 176 126 85 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3008 844 Size: 2164 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3540 1394 Size: 429 1717 3507 Fragments arranged by size: 3507 1717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4148 4322 65 Size: 1183 2900 174 1396 Fragments arranged by size: 2900 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4297 4484 1700 Size: 110 2487 187 2869 Fragments arranged by size: 2869 2487 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5653 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 3713 4725 3713 Size: 1012 4641 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5653 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5653 BstXI CCAn_nnnn'nTGG Cuts at: 2372 2372 Size: 5653 BstYI r'GATC_y Cuts at: 1972 2904 2983 3350 3735 3752 5204 5290 5302 Size: 932 79 367 385 17 1452 86 12 Cuts at: 5302 5648 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 932 385 367 346 86 79 17 12 BtgI C'CryG_G Cuts at: 513 1065 2908 513 Size: 552 1843 3258 Fragments arranged by size: 3258 1843 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4914 Fragments arranged by size: 4914 683 56 BtsI GCAGTG_nn' Cuts at: 2424 3169 3963 3983 2424 Size: 745 794 20 4094 Fragments arranged by size: 4094 794 745 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2713 2748 529 Size: 35 2149 35 3434 Fragments arranged by size: 3434 2149 35 35 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2217 2362 2522 Size: 54 1425 50 275 18 387 145 160 Cuts at: 2522 2838 3100 3989 5618 8 Size: 316 262 889 1629 43 Fragments arranged by size: 1629 1425 889 387 316 275 262 160 145 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 2858 3583 1353 Size: 848 657 725 3423 Fragments arranged by size: 3423 848 725 657 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4240 4614 4760 387 Size: 1064 138 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2721 3716 4313 5100 5368 1239 Size: 393 461 628 995 597 787 268 1524 Fragments arranged by size: 1524 995 787 628 597 461 393 268 EcoRI G'AATT_C Cuts at: 2962 3340 2962 Size: 378 5275 EcoRV GAT'ATC Cuts at: 2094 2094 Size: 5653 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2321 2321 Size: 5653 FspI TGC'GCA Cuts at: 4159 4159 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 4800 1678 Size: 3122 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2440 2472 4294 4326 4368 Size: 32 135 32 868 32 1822 32 42 Cuts at: 4368 4400 1373 Size: 32 2626 Fragments arranged by size: 2626 1822 868 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2593 2997 3243 669 Size: 988 281 655 404 246 3079 Fragments arranged by size: 3079 988 655 404 281 246 HindIII A'AGCT_T Cuts at: 748 2298 748 Size: 1550 4103 HpaI GTT'AAC Cuts at: 2593 3243 2593 Size: 650 5003 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4767 4951 1305 Size: 3462 184 2007 Fragments arranged by size: 3462 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2364 2524 2840 10 Size: 54 1425 343 532 160 316 2823 Fragments arranged by size: 2823 1425 532 343 316 160 54 MslI CAynn'nnrTG Cuts at: 518 1166 2745 3611 3970 4129 518 Size: 648 1579 866 359 159 2042 Fragments arranged by size: 2042 1579 866 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2352 3069 3746 4894 5139 1606 Size: 746 717 677 1148 245 2120 Fragments arranged by size: 2120 1148 746 717 677 245 NcoI C'CATG_G Cuts at: 513 1065 2908 513 Size: 552 1843 3258 Fragments arranged by size: 3258 1843 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 2733 3011 4556 1132 Size: 97 1504 278 1545 2229 Fragments arranged by size: 2229 1545 1504 278 97 PasI CC'CwG_GG Cuts at: 1348 2066 2431 1348 Size: 718 365 4570 Fragments arranged by size: 4570 718 365 PciI A'CATG_T Cuts at: 2729 4552 2729 Size: 1823 3830 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5653 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PstI C_TGCA'G Cuts at: 830 3005 830 Size: 2175 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 PvuII CAG'CTG Cuts at: 2352 2352 Size: 5653 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 3901 5613 3901 Size: 1712 3941 SfcI C'TryA_G Cuts at: 826 1045 2250 2393 2787 2884 3001 4136 4817 Size: 219 1205 143 394 97 117 1135 681 Cuts at: 4817 5008 826 Size: 191 1471 Fragments arranged by size: 1471 1205 1135 681 394 219 191 143 117 97 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3757 4658 4920 820 Size: 197 926 1814 901 262 1553 Fragments arranged by size: 1814 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 2131 2800 2898 2908 3092 513 Size: 552 1066 669 98 10 184 3074 Fragments arranged by size: 3074 1066 669 552 184 98 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3680 3697 3850 4035 4480 325 Size: 1255 2100 17 153 185 445 1498 Fragments arranged by size: 2100 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3899 5611 96 Size: 274 80 33 51 1443 1922 1712 138 Fragments arranged by size: 1922 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2796 2828 5613 5645 2796 Size: 32 2785 32 2804 Fragments arranged by size: 2804 2785 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5653 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2212 2212 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI